University of Tabriz Journal of Agricultural Science and Sustainable Production 2476-4310 36 1 2026 04 20 The effect of inoculation with biofertilizers on the quantitative and qualitative characteristics of the medicinal plant Hyssopus officinalis at different levels of water deficit stress The effect of inoculation with biofertilizers on the quantitative and qualitative characteristics of the medicinal plant Hyssopus officinalis at different levels of water deficit stress 75 88 21413 10.22034/saps.2024.62497.3251 FA Masoumeh Shahi Department of Agronomy and Plant Breeding, Tabriz Branch, Islamic Azad University, Tabriz, Iran Mahmoud Pouryousef Miandoab Department of Agronomy and Agroecology, Urmia Branch, Islamic Azad University, Urmia, Iran Mehrdad Yarnia Dept., of Agronomy and Plant Breeding, Tabriz Branch, Islamic Azad University, Tabriz, Tabriz, Iran. Ebrahim Khalilvand Behrouzyar Department of Agronomy and Plant Breeding, Tabriz Branch, Islamic Azad University, Tabriz, Iran Abdollah Hassanzadeh Ghorttapeh Horticulture Crop Science Research Department, West Azarbaijan Agricultural and Natural Resources Research and Education Center, (AREEO) ,Urmia, Iran. Journal Article 2024 07 14 Background and Objectives: In arid and semi-arid regions, employing innovative and sustainable solutions to enhance nutrient uptake is crucial. One such approach is the utilization of microorganisms.This research investigated the effect of mycorrhizae and growth-stimulating bacteria on hyssop's quantitative and qualitative characteristics under different irrigation levels. Materials and Methods: The experiment was conducted as a split plot based on a randomized complete block design with three replications at the West Azarbaijan province research centre (Saatlo station) over two crop years. The irrigation levels, including normal moisture conditions (7-day interval), mild stress (10-day interval), and severe stress (14-day interval), were assigned to the main plots, and inoculation with mycorrhiza (My), Azotobacter (Az), Pseudomonas (Pse), Az + Ps, My+Az+Pse and non-inoculation treatment (control) were assigned to sub-plots. In this experiment, the quantitative and qualitative characteristics of the plant were measured. Results: The highest plant height, total fresh weight, and leaf nitrogen content were assigned to the treatment with Pseudomonas (Pse) under normal irrigation conditions. The maximum essential oil percentage and essential oil yield were related to Psetreatment under mild stress conditions. In all biofertiliser treatments, plant height, total fresh weight, chlorophyll a and b, carotenoid content, nitrogen, phosphorus, and potassium content of leaves decreased with increasing water stress. In contrast, proline content and ion leakage increased. Inoculation with My+Az+Pse under mild and severe stress conditions increased plant height, shoot weight, chlorophyll a content, carotenoid, proline, nitrogen, phosphorus, and essential oil yield. This treatment also reduced the amount of ion leakage compared to control. Conclusion: The separate application of each of the Pse, My, and Az treatments under mild stress conditions resulted in obtaining the maximum essential oil yield, and the combined application of My+Az+Pse adjusted water stress on the essential oil yield under severe water deficit conditions. Background and Objectives: In arid and semi-arid regions, employing innovative and sustainable solutions to enhance nutrient uptake is crucial. One such approach is the utilization of microorganisms.This research investigated the effect of mycorrhizae and growth-stimulating bacteria on hyssop's quantitative and qualitative characteristics under different irrigation levels. Materials and Methods: The experiment was conducted as a split plot based on a randomized complete block design with three replications at the West Azarbaijan province research centre (Saatlo station) over two crop years. The irrigation levels, including normal moisture conditions (7-day interval), mild stress (10-day interval), and severe stress (14-day interval), were assigned to the main plots, and inoculation with mycorrhiza (My), Azotobacter (Az), Pseudomonas (Pse), Az + Ps, My+Az+Pse and non-inoculation treatment (control) were assigned to sub-plots. In this experiment, the quantitative and qualitative characteristics of the plant were measured. Results: The highest plant height, total fresh weight, and leaf nitrogen content were assigned to the treatment with Pseudomonas (Pse) under normal irrigation conditions. The maximum essential oil percentage and essential oil yield were related to Psetreatment under mild stress conditions. In all biofertiliser treatments, plant height, total fresh weight, chlorophyll a and b, carotenoid content, nitrogen, phosphorus, and potassium content of leaves decreased with increasing water stress. In contrast, proline content and ion leakage increased. Inoculation with My+Az+Pse under mild and severe stress conditions increased plant height, shoot weight, chlorophyll a content, carotenoid, proline, nitrogen, phosphorus, and essential oil yield. This treatment also reduced the amount of ion leakage compared to control. Conclusion: The separate application of each of the Pse, My, and Az treatments under mild stress conditions resulted in obtaining the maximum essential oil yield, and the combined application of My+Az+Pse adjusted water stress on the essential oil yield under severe water deficit conditions.

https://sustainagriculture.tabrizu.ac.ir/article_21413_baad7a2abc27bbb726e8d98f5ca80934.pdf