Effect of Salicylic Acid and Glycine Betaine Foliar Application on Morpho-Physiological Characteristics of Carla (Momordica charantia L.) under Water Deficit Stress

Document Type : Research Paper

Authors

1 Master Student of Medicinal Plants, Department of Horticultural Sciences, Faculty of Agriculture, University of Zanjan. iran

2 Assistant professorDepartment of Horticultural Sciences, Faculty of Agriculture, University of Zanjan, iran

3 Assistant Professor Department of Horticultural Sciences, Faculty of Agriculture, University of Zanjan. iran

4 Assistant professor Department of Horticultural science, Faculty of Agriculture, University of Zanjan, iran

Abstract

The effect of salicylic acid and glycine betaine foliar application on morpho-physiological characteristics of Carla (Momordica charantia L.) under water deficit stress, were studied by an experiment as split plot based on randomized complete block design with three replications in 2017 at Zanjan University Research Farm. The treatments consisted of three levels of irrigation, 50, 75 and 100% of field capacity as the main factor and two levels of salicylic acid (2 and 4 m M), two levels of glycine betaine (50 and 100 m M) as Sub factors were used. The results showed that the increase in water deficit stress significantly reduced the total chlorophyll content, vitamin C content, seed weight, plant length and number of fruits per plant. But increasing in water deficit stress caused a significant increase in the amount of proline and Ion leakage. The application of salicylic acid and glycine betaine increased the Ion leakage and total chlorophyll content, vitamin C, proline, plant length, number of fruits per plant, but did not have a significant effect on seed weight. In general, due to the fact that the use of salicylic acid and glycine betaine have ability to improve the morphological and physiological traits of treated plants. It seems that application of these treatments can increase Carla tolerance to water deficit stress.
 

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References

A. O. A. C, 2000. Association of official analytical chemists. Washington D. C, 12: 377-378.
Alfonso LV and Martin Mex R, 2007. Effect of Salicylic Acid on the Bio productivity of Plant. Salicylic Acid-a Plant Hormone. Pp 15-23.
Arakawa T and Timasheff SN, 1983. Preferential interactions of proteins with solvent components in aqueous amino acid solutions. Archives of Biochemistry and Biophysics, 224: 169–177.
Arnon AN, 1967. Method of extraction of chlorophyll in the plants. Agronomy Journal, 23:112-121
Arvin MJ, Beidshki A, Kramt B and Maghsodi K, 2011. The study salicylic acid (SA) role in contrast with drought stress by affecting on morphological and physiological parameters in garlic plant. In: Proceeding of 7th Iranian Horticultural Science Congress, Isfahan Industrial University, Iranian 4-7 September 2011. (In Persian).
Ashraf M and Foolad M.R, 2007. Roles of glycine betaine and proline in improving plant abiotic stress resistance. .Environmental and Experimental Botany, 59: 206–216.
Ashraf M, 2009. Biotechnological approach of improving plant salt tolerance using antioxidants asmarkers. Biotechnology Advance, 27, 84-93.
Ashraf M, Akram NA, Arteca RN and Foolad MR, 2010. The physiological, biochemical and molecular roles of brassino steroids and salicylic acidin plant processes and salt tolerance. Plant Science, 29: 162–190.
Bates LS, Waklren RP and Trare ID, 1973. Rapid determination of free proline water stress studies. Plant Soil, 39: 205-207
Bayat H, Men H, Aryan H and weaponization J, 2012. Effect of salicylic acid on morphological and physiological characteristics of cucumber seedlings (Cucumis sativus cv. Super Dominus) under drought stress. Plant Production Research, 3: 14-1. (In Persian).
Bayoumi TY, Eid M and Metwali EM, 2008. Application of physiological and biochemical indicesas a screening technique for drought tolerance in wheatgenotypes. African Jornal of Biotechnology, 7: 2341-2352.
Beltrano J and Ronco MG, 2008. Improved tolerance of wheat plants (Triticum aestivum L.) to drought stress and re watering by the arbuscular mycorrhizal fungus Glomus claroideum: Effect on growth and cell membrane stability. Brazilian Journal of Plant Physiology, 20:29-37.
Crisan S, Campeanu G and Halmagean L, 2008. Study of Momordica charantia L. species grown on the specific conditions of Romania’s western part. Vegetable Growing, 425-428.
Dalire R, Sharpor C, esfandiari A and Seyed Sharifi R, 2010. Evaluation of different ecotypes of marigold in terms of drought tolerance in hydroponic culture media. Science and technology of greenhouse cultivation, 1: 9-17. (In Persian).
Dolphani M, Barry M, Maleki Maleki Fr, Safari Karazar M, Qazi-zadeh M, Masoumi A and Junjani S, 2016. The role of glycine betaine in the improvement of tolerance to drought stress in plants. 13 th National Conference on Irrigation and Evaporation, Shahid Bahonar University, Kerman. (In Persian).
Gibon YA, Bessieres M and Larher F, 1997. Is glycine betaine a non ompatible solute in higher plants do not accumulate it? Plants Cell and Environment, 20: 329-340
Gorham J, Jokinen K, Malik MNA, Khan IA, 2000. Glycine betaine treatment improves cotton yields in field trials in Pakistan. Proceedings of the World Cotton Research Conference II, Athens, Greece.
Hassani A, Omidbaigi R and Heidari Sharif Abad H, 2004. Study of some drought resistance indices in basil (Ocimum basilicum). Agricultural Science Natural Resources, 10: 65-74. (InPersian).
Hayat Q, Hayata SH, Irfan M and Ahmad A, .2010. Effect of exogenous salicylic acid under changing environment. A review. Environmental and Experimental Botany, 68: 14-25.
Heydari Sharifabad H, 2002. Plant and salinity. Forestry and Rangeland Research Institute publications. 34-36. (In Persian).
Hoque M A, Banu M N A, Nakamura Y, Shimoishi Y and Murata Y, 2008. Proline and glycine betaine enhance antioxidant defense and methylglyoxal detoxification systems and reduce NaCl-induced damage in cultured tobacco cells. Plant Physiology, 165: 813– 824.
Ismail AM and Hall AE. 1999. Reproductive-stage heat tolerance, leaf membrane thermo-stability and plant morphology in cowpea. Crop Science, 39: 1762-1768.
Jahangir M, Bayoumi Abdel-Farid I, Kim H, Chio Y and Verpoorte R, 2009. Healthy and unhealthy plants. The effect of stress on the metabolism of Brassicaceae. Environmental and Experimental Botany, 67: 23-33.
Kalarani MK, Thangaraj M, Sivakumar R and Mallika R, 2002. Effects of salicylic acid on tomato (Lycopersicon esculentum Mill) productivity. Crop Research, 23 (3): 486-492.
Korkmaz A, Sirikci R, Kocacınar F, Deger O and Demirkırıan AR, 2012. Alleviation of salt-induced adverse effects in pepper seedlings by seed application of glycine betaine. Scientia Horticulturae, 148: 197- 205.
Linster CL and Clarke SG, 2008. L-Ascorbate biosynthesis in higher plants: the role of VTC2. Trends in Plant Science 13 (11): 567- 573.
Ma XL, Wang YJ, Xie SL, Wang C and Wang W, 2007. Glycine betaine application ameliorates negative effects of drought stress in tobacco. Plant Physiology, 54: 472–479.
Margarita M, Crosby KM and Eliezer S, 2002. Differential gene expression analysis in melon roots under drought stress conditions. Subtropical Plant Science, 54: 6-10.
Massacci A, Nabiev SM, Pietrosanti L, Nematov S K, Chernikova T N, Thorand K and Leipner J, 2008. Response of the photosynthetic apparatus of cotton (Gossypium hirsutum) to the onset of drought stress under field condition sstudied by gas-exchange analysis and chlorophyll fluorescence imaging. Plant Physiology and Biochemistry, 46 (2): 189–195.
Mirabdollahi SM. 2011. The Changes of Essential Oil Yield and Composition of Fennel under Limited Irrigation Condition. MSc .thesis University of Zanjan.
Mirie H.R and Zamani Moghaddam A 2015. External application of glycine betaine to reduce the effects of drought stress in corn) Zea mays (. Iranian Journal of Field Crops Research, 12(4), 704-717. (In Persian).
Mohtashmi F, Puriusf M, Andalibi B and Hatching Feb, 2015. Effect of soluble and pre-treatment pretreatment with salicylic acid on the yield and essential oils of Fennel under drought stress conditions. Iranian Journal of Medicinal Plants and Herbs Research Journal of Dermatology, 3(5):841-852. (In Persian).
Popova  L, Ananieva V, Hristova V, Christov K, Georgieva K, Alexieva V and Stoinova  Zh,  2003. Salicylic acid-and Methyljasmonate induced protection on photosynthesis to paraquat oxidative stress. Bulgarian Journal of Plant Physiology, special issue, 133-152.
Pospisilova J, 2011. Responses of Transgenic Tobacco Plants with Increased Proline Content to Drought and/or Heat Stress. Plant Sciences, 2: 318–324
Reza SH, Athar HUR and Ashraf M, 2006. Influence of exogenously applied glycine betaine on the photosynthetic capacity of two differently adopted wheat cultivars under salt stress. Botany, 38: 241-251.
Sairam RK, Rao KV, Srivastava GC,.2002. Differential response of wheat genotypes to long-term salinity stress in relation to oxidative stress, antioxidant activity and osmolyte concentration. Plant Science, 163:1037-1046.
Sarkar S, Pranava M and Marita R, 1996. Demonstration of the hypoglycemic action of Momordica charantia in a validated animal model of diabetes. Pharmacological Research, 33 (1):1-4.
Sat Pal Sharmaa I, Leskovara D, Kevin AM, Crosbyb AMH, and Astrid Volderb I, 2014. Root growth, yield and fruit quality responses of reticulatus and inodorus melons (Cucumis melo L.) to deficit subsurface drip irrigation. Agricultural Water Management, 136 (C): 75–85.
Savari A, Fotokian M and Barzali M, 2009. Evaluation of glycine betaine effects on some agronomic traits of cotton (Gossypium hirsutum L.) cultivars under water-drought stress. Journal of Daneshvar Agronomy Sciences.1 (1):67-76. (In Persian).
Senaranta T, Ouchell D, Bunn E, and Dixon K, 2000. Acetyl salicylic acid (aspirin) and salicylic acid induce multiple stress tolerance in bean and tomato plants. Plant Growth Regulation, 30: 157-161.
Shibli RA, Kushad M, Yousef GG and Lila MA, 2007. Physiological and biochemical responses of tomato micro shoots to induced salinity stress with associated ethylene accumulation. Plant Growth Regulation, 51 (2): 159-169.
Shishido Y, Yahashi T, Seyama N and Imada S, 1992. Effects of leaf position and water management on translocation and distribution of14C assimilates in fruiting muskmelons. Japanese Society Horticultural Sciences, 60 (4): 897-903
Stevens J, Senaratna T and Sivasithamparam K, 2006. Salicylic acid induces salinity tolerance in Tomato (Lycopersicon esculentum cv. Roma): associated changes in gas exchange, water relations and membrane stabilization. Plant Growth Regulation, 49(6), 77-83
Yazdanpanah S, Abasi F and Baghzadeh A, 2010. Effect of salicylic acid and ascorbic acid on proline, sugar and protein content in Satureja hortensis L. under aridity stress. Proceeding of the First National Conference of Environmental Stress in Agricultural Science, 28-29.
JOURNAL OF AGRICULTURAL SCIENCE AND SUSTAINABLE PRODUCTION
Volume 29, Issue 1
April 2019
Pages 223-235

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