Enriching Vermicompost by Nitrogen Fixing and Phosphate Solubilizing Bacteria

Document Type : Research Paper

Authors

Abstract

Changes in populations of microorganisms in the vermicompost in order to improving its quality has been the novel study topics in the field of organic fertilizer production. Present study was performed with aimed to possibility of changing in the content of nitrogen and phosphorus in the vermicompost and to determine some quality characteristics in vermicompost. In order to accomplish this goal, after the production of vermicompost, inoculums from Pseudomonas and Azotobacter bacteria genera were prepared, then bacteria inoculated to vermicompost beds, for 60 days at a temperature of 28 ° C storage, and biological and chemical factors were measured on days 0, 20, 40 and 60 in vermicompost. Results showed that with increasing incubation time, the population of bacteria, nitrogen, available phosphorus, ash and humic acid increased, and the amount of organic carbon, EC and pH decreased. During incubation, Azotobacter and Pseudomonas treatments increased N content about 80 and 20 percent and P content about 25 and 40 percent, respectively. According to results of this study, 45 to 60 day incubation period could have very good results on the chemical and biological properties in vermicompost, but this effect is not significant in less than 40 days and more than 60 days. Bacteria which used in the present study had beneficial and different effects and depending the requirement could be used for vermicompost enrichment.

Keywords

References

Arancon NQ, Edwards CA, Atiyeh RM and Metzger JD, 2004. Effects of vermicomposts produced from food waste on greenhouse peppers. Bioresour. Technol. 93, 139–144.
Beauchamp  CJ, Levesque G, Prevost D and Chalifour FP, 2006. Isolation of free-living dinitrogen-fixing bacteria and their activity in compost containing de-inking paper sludge. Biores. Technol. 97, 1002– 1011.
 
Busato JG, Lima LS, Aguiar NO, Canellas LP and Olivares FL, 2012. Changes in labile phosphorus forms during maturation of vermicompost enriched with phosphorus-solubilizing and diazotrophic bacteria. Bioresource Technology. Accepted.
Eivazi F and Tabatabai MA, 1977. Phosphatases in soils. Soil Biol. Biochem. 9, 167–172.
Elvira C, Goicoechea M, Sampedro L, Mato S and Nogales R, 1996. Bioconversion of solid paper-pulp mill sludge earthworms. Biores. Technol. 57, 173–177.
Elvira C, Sampedro L, Benitez, E and Nogales R, 1998. Vermicomposting of sludges from paper-mill and dairy industries with Eisenia andrei: a pilot-scale study. Biores. Technol. 63, 205–211.
Fischer K, Hahn D, Honerlage W and Zeyer J, 1997. Efect of passage through the gut of earthworm Lumbricus terrestris L. on Bacollus megaterium studied by whole cell hybridisation. Soil Biol. Biochem. 29, 1149–1152.
Fracchia L, Dohrmann AB, Martinotti MG and Tebbe CC, 2006. Bacterial diversity in a finished compost and vermicompost: differences revealed by cultivationindependent analyses of PCR-amplified 16S rRNA genes. Appl. Microbiol. Biotechnol. 71, 942–952.
Frossard E, Sinaj S and Dufour P, 1996. Phosphorus in sewage sludges as assessed by isotopic exchange. Soil Sci. Soc. Am. J. 60, 179–182.
Gupta pk, 2008. Vermicomposting for sustainable agriculture. Agrobios (India) Publishers, Jodhpur, 224pp.
Gutiérrez-Miceli FA, Moguel-Zamudio B, Abud-Archila M, Gutiérrez-Oliva VF and Dendooven L, 2008. Sheep manure vermicompost supplemented with a native diazotrophic bacteria and mycorrhizas for maize cultivation. Bioresour. Technol. 99, 7020–7026.
Hendrikson NB, 1990. Leaf litter selection by detritivore and geophagous earthworms. Biol. Fertil. Soils 10, 17–21.
Kaushik P and Garg VK, 2004. Dynamics of biological and chemical paramerers durimg vermicomposting of solid textiler mill sludges mixed with cow dung and agricultural residues. Biores. Technol. 4, 203–209.
Kaushik P, Yadav YK, Dilbaghi N and Garg VK, 2008. Enrichment of vermicomposts prepared from cow dung spiked solid textile mill sludge using nitrogen fixing and phosphate solubilizing bacteria. Environmentalist, 28:283–287.
Kumar V and Narula N, 1999. Solubilization of inorganic phosphates and growth emergance of wheat as a.ected by Azotobacter chroococcum. Biology and Fertility of Soils 28, 301-305.
Kumar V and Singh KP, 2001. Enriching vermicompost by nitrogen fixing and phosphate solubilizing bacteria. Bioresource Technology 76, 173-175.
Le Bayon RC and Binet F, 2006. Earthworms change the distribution and availability of phosphorous in organic substrates. Soil Biol. Biochem. 38, 235–246.
Mohammady Aria M, Lakzian A, Haghnia GH, Berenji AR, Besharati H and Fotovat A, 2010. Effect of Thiobacillus, sulfur, and vermicompost on the water-soluble phosphorus of hard rock phosphate. Bioresour. Technol. 101, 551–554.
Padmavathiamma PK, Li LY and Kumari UR, 2008. An experimental study of vermibiowaste composting for agricultural soil improvement. Bioresour. Technol. 99, 1672–1681.
Page AL, 1982. Methods of Soil Analysis. Agronomi 9, ASA, SSSA, Madison, Wiscosin, USA.
Parle JN, 1963. A microbiological study of earthworm cast. Journal of General Microbiology, 31, 13–22.
Pramanik P, Ghosh GK, Ghosal PK and Banik P, 2007. Changes in organic – C, N, P and K and enzyme activities in vermicompost of biodegradable organic wastes under liming and microbial inoculants. Bioresource Technology 98, 2485–2494.
Premono EM, Moawad MA and Vlek PLG, 1996. E.ect of phosphate-solubilizing Pseudomonas putida on the growth of maize and its survival in the rhizosphere. Indonesian Journal of Crop Science 11, 13-23.
Qi BC, Aldrich C and Lorenzen L, 2004. Effect of ultrasonication on the humic acids extracted from lignocellulose substrate decomposed by anaerobic digestion. Chemical Engineering Journal 98, 153–163.
Reinecke AJ, Viljioen SA and Saayman RJ, 1992. The suitability of Eudrilus eugeniae, Perionyx excavatus and Eisenia fetida (Oligochaete) for vermicomposting in southern Africa in terms of their temperature requirements. Soil Biol. Biochem. 24, 1295–1307.
Scervino JM, Mesa MP, Mónica ID, Recchi M, Moreno NS and Godeas A, 2010. Soil fungal isolates produce different organic acid patterns involved in phosphate salts solubilization. Biol. Fertil. Soils 46, 755–763.
Stevenson FJ, 1994. Humic Chemistry: Genesis, Composition, Reactions, 2nd ed. Wiley, New York.
Tiwari SC, and Mishra RR, 1993. Fungal abundance and diversity in earthworm cast and in uningested soil. Biology and Fertility of Soils, 16, 131–134.
Troussellier M, Bonnefont JL, Courties C, Derrien A, Dupray E, Gauthier M, Gourmelon M, Joux F, Lebaron P, Martin Y and Pommepuy M, 1998. Responses of enteric bacteria to environmental stresses in seawater. Oceanologica Acta. Vol. 21 - N” 6.
Veeken A, Nierop K, Wilde Vd, Hamelers B, 2000. Characterisation of NaOH-extracted humic acids during composting of a biowaste. Bioresource Technology 72, 33-41.
Yates L M, Engebreston RR and Haakenson TJ, 1997. R. Von wandruszka, Anal. Chim. Acta 356, 295–301.
 
 

Files

Share

How to cite

Statistics