Effects of Modified EDDHA iron synthesized Complexes on Agronomic Traits and Yield of bean (Phaseolus vulgaris L.) in Calcareous Soils

Document Type : Research Paper

Authors

1 Ph.D. student in production and post-harvest physiology of horticultural plants, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.

2 Department of Horticulture, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.

3 Department of Eco-Physiology, Faculty of Agriculture, Tabriz University, Tabriz, Iran.

Abstract

Background and Objectives: Beans is one of the most important legumes, which has a major contribution to the human diet. It also has a great ability to bio-fix soil nitrogen, and its branches and leaves are also used as animal feed. It has a high protein content and contains essential amino acids, iron, and excellent quality and taste. Iron is one of the essential and micro-elements that plays an important role in soil fertility processes, carbon dioxide stabilization, and increasing product quality, and it is the most important micro-element whose deficiency has a direct impact on the human diet. To solve the problem of iron absorption in calcareous soils, iron complexes using modified EDDHA organic chelators can be used. Therefore, this study was conducted to investigate the role of modified EDDHA iron synthesis complexes in improving the growth and yield of beans (Phaseolus vulgaris L.) in calcareous soils.
 
Materials & Methods: This experiment was conducted as a factorial based on a randomized complete block design with three replications in the research greenhouse of the Department of Horticultural Science and Engineering, University of Tabriz. Treatments included three levels of calcareous soil (S1, S2, and S3 with 5, 10, and 15% lime, respectively) and five levels of iron (F0, F1, F2, F3, and F4 as a control, EDDHA with acyl chlorides of aliphatic acids; two EDDHA with three amido phenols; EDDHA with aminophenol; and EDDHA with oleic acid epoxide, respectively). Iron complexes with modified EDDHA organic chelating agents were synthesized and used for iron micronutrient feeding to plants.
 
Results: The highest activity of (Fv/Fo) was observed with the synthesis of modified irons with 10% soil lime, the highest soil EC with 15% soil lime in the EDDHA treatment with acyl chlorides of aliphatic acids 2, and the highest soil pH with 10% lime and no use of modified EDDHA. EDDHA caused a decrease in soil pH at different percentages of lime. EDDHA combined with oleic acid epoxide (F4) produced the highest fresh and dry weight of bean roots. The synthesis of various modified irons in soil with 5% lime increased grain yield.
 
Conclusion: The study found that a 10% calcareous soil had the highest effectiveness when plants were treated with EDDHA (F4) combined with oleic acid epoxide, which enhanced iron absorption and improved photosynthesis. This modification led to a 10% increase in bean grain yield.
 

Keywords

Main Subjects


 
Abadia J, Eugenio A, Tsipouridis C, Bruggemann W, Neguerole J and Marangoni B. 1998. Novel approaches for the control of iron cholorosis in fruit tree crops, Final Report, 144 p.
Ali Dib T, Monneveux PH, Acevedo J and Nachil M. 1994. Evaluation of proline analysis and chlorophyll fluorescence quenching measurements as drought tolerance indicator in durum wheat (Triticum turgidum L.). Euphytica, 79: 65-73.
Bavaresco L and Poni S. 2003. Effect of calcareous soil on photosynthesis rate, mineral nutrition, and source- sink ratio of table grape. Journal of Plant Nutrition, 5: 747-753. https://doi.org/10.1081/PLN-120024269
Bozorgi HR. 2012. Study effects of nitrogen fertilizer management under nano iron chelate foliar spraying on yield and yield components of eggplant (Solanium melongena L.). ARPN Journal of Agricultural and Biological Science, 7(4): 233-237.
Caballero R, Ordovás J, Pajuelo P, Carmona E and Delgado A. 2007. Iron Chlorosis in Gerber as Related to Properties of Various Types of Compost Used as Growing Media. Communications in Soil Science and Plant Analysis, 38: 2357-2369. https://doi.org/10.1080/00103620701588494
Chohura P, Kołota E and Komosa A. 2007. The effect of different source of iron on nutritional value of greenhouse tomato fruit grown in peat substrate. Journal of Fruit and Ornamental Plant Research, 67(1): 55-61. DOI: 10.2478/v10032-007-0030-8
Elkins R and Fichtner E. 2016. Causes and Control of Lime-induced Fe Deficiency in California Fruit and Nut Crops. University of California Agriculture and Na tural Resources Publication, 21637.
Garnica M, Bacaicoa E, Mora V, San Francisco S, Baigorri R, Zamarreño AM and Garcia-Mina JM. 2018. Shoot iron status and auxin are involved in iron deciency-induced Phyto siderophores release in wheat. BMC Plant Biology, 18(1):105. https://doi.org/10.1186/s12870-018-1324-3
Genty B, Briantais JM and Baker NR. 1989. The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. Biochimica et Biophysica Acta (BBA)-General Subjects, 99: 87–92. https://doi.org/10.1016/S0304-4165(89)80016-9
Gülser F, Yavuz Hİ, Gökkaya TH and Sedef M. 2019. Effects of iron sources and doses on plant growth criteria in soybean seedlings. Eurasian Journal of Soil Science, 8(4): 298-303. https://doi.org/10.18393/ejss.582231
Karimian M, Mir B, Bidranameni F and Keshtehgar A. 2020. Effects of Manure and Different Intercropping Patterns on Quantitative and Qualitative Yield of Roselle (Hibiscus Sabdariffa) and Cowpea (Phasaeolous vulgaris). Crop Science Research in Arid Regions, 2(1): 113-125 (In Persian).  https://doi.org/10.22034/csrar.2020.119091
Kim SA and Guerinot ML. 2007. Mining Iron: Iron Uptake and Transport in Plants. FEBS Letters, 581: 2273-2280. https://doi.org/10.1016/j.febslet.2007.04.043
Li J, Cao X, Jia X, Liu L, Cao H, Qin W and Li M. 2021. Iron decency leads to chlorosis through impacting chlorophyll synthesis and nitrogen metabolism in Areca catechu L. Frontiers in Plant Science, 12. https://doi.org/10.3389/fpls.2021.710093
Lucena J, De Aberasturi MJ and Gárate A. 1991. Stability of chelates in nutrient solutions for drip irrigation. Iron Nutrition and Interactions in Plants. Netherlands Springer, 63-67. https://doi.org/10.1007/978-94-011-3294-7_7
Moghadam A, Vattani H, Baghaei N and Keshavarz N. 2012. Effect of Different Levels of Fertilizer Nano_Iron Chelates on Growth and Yield Characteristics of Two Varieties of Spinach (Spinacia oleracea L.): Varamin 88 and Viroflay. Research Journal of Applied Sciences. Engineering and Technology, 4(22): 4813-4818.‏
Murgia I, Arosio P, Tarantino D and Soave C. 2012. Biofortification for combating ‘hidden hunger’ for iron. Trends In Plant Science, 17: 47-55.
Ngan HTM, Tung HT, Le BV annd Nhut DT. 2020. Evaluation of root growth, antioxidant enzyme activity
and mineral absorbability of carnation (Dianthus caryophyllus “Express golem” cultured in two culture systems supplemented with iron nanoparticles. Scientia Horticulturae, 272(15): 109612.
https://doi.org/10.1016/j.scienta.2020.109612
Pandey AC, Sanjay S and Yadav R. 2010. Application of ZnO nanoparticles in influencing the growth rate of Cicer arietinum. Journal of Experimental nanoscience, 5(6): 488-497.‏ https://doi.org/10.1080/17458081003649648
Peyvandi M, Parande H and Mirza M. 2011. Comparison of Nano Fe Chelate with Fe Chelate Effect on Growth Parameters and Antioxidant Enzymes Activity of Ocimum basilicum. New Cell Mol Biotechnology, 1(4): 1-12. http://dorl.net/dor/20.1001.1.22285458.1390.1.4.3.7
Pinto A, Mota Ad, De Varennes A and Pinto F. 2004. Influence of Organic Matter on the Uptake of Cadmium, Zinc, Copper and Iron by Sorghum Plants. Science of the Total Environment, 326: 239-247. https://doi.org/10.1016/j.scitotenv.2004.01.004
Prasad TN, Sudhakar P, Sreenivasulu Y, Latha P, Munaswamy V, Raya Reddy K, Sreeprasad TS, Sajanlal PR and Pradeep T. 2012. Effect of nano scales zink oxide on the germination, growth and yield of peanut. Journal Plant Nutrition, 35(1): 905-927.
Rojas CL, Romera FJ, Alcantara E, Perez-Vicente R, Sariego C, García-Alonso JI and Marti G. 2008. Efficacy of Fe (o, o-EDDHA) and Fe (o, p-EDDHA) isomers in supplying Fe to strategy I plants differs in nutrient solution and calcareous soil. Journal of agricultural and food chemistry, 56(22): 10774-10778.‏ https://doi.org/10.1021/jf8022589
Voysest O. 2000. Breeding of beans (Phaseolus Vulgaris L.) Legacy of Latin American Varieties 1930- (1999). Cali, Valle, Colombia: International Center for Tropical Agriculture.
Xia A, Li Y and Zou D. 2004. Effects of salinity stress on PSII in Ulva lactuca as probed by chlorophyll
fluorescence measurements. Aquatic Botany, 8: 129-137. http://dx.doi.org/10.1016/j.aquabot.2004.07.006.
Zuluaga MYA, Cardarelli M, Rouphael Y, Cesco S, Pii Y and Colla G. 2023. Iron nutrition in agriculture: From synthetic chelates to bio-chelates. Scientia Horticulturae, 312: 111833. https://doi.org/10.1016/j.scienta.2023.111833.