Effect of Salinity Stress, Treahalose and Endophytic fungus Serendipita indica on some Physiological and Biochemical Traits of tomato

Document Type : Research Paper

Authors

1 Department of Plant Production and Genetics, Faculty of Agriculture, Malayer University, Malayer, Iran

2 Department of Agronomy and Plant Breeding, Faculty of Agriculture, Malayer University, malayer, Iran

3 Plant Production and Genetics department, Faculty of Agriculture Malayer University Malayer Iran

Abstract

Background & Objective: Abiotic-stress tolerance and plant growth enhancement can be evoked in crops by the exploitation of beneficial microorganisms. The effect of inoculation with Serendipita indica and treahalose on germination, growth and morphophysiological characteristics of tomato plant under salinity stress was the goal of the present experiment.
 
Materials & Methods: In this research, the interaction effect of S. indica, NaCl (0, 50 and 100 mM) and trehalose (0, 25 and 50 mM) on germination of tomato, also the interaction effect of S. indica, NaCl and trehalose in pot were investigated on morphophysiological traits of tomato according to a factorial experiment based on completely randomize design (CRD) with 3 replications in two independent experiments. In germination experiment, after disinfection, seeds were placed in spore of S. indica  for 24 hours. Then, sodium chloride and trehalose treatments were applied to the inoculated plants. All treatments and control, were placed in a germinator at 25°C and 45% relative humidity, and germination and related traits were evaluated. In pot experiment, inoculation was performed 48 hours after sowing the seeds in the germinator. Then, the inoculated seeds were transferred to pots and salt stress and trehalose treatment were applied. After 60 days, the plants were harvested and morphophysiological traits were measured.
 
Results: In germination experiment the results of ANOVA showed that the main effects and interactions of salinity stress,  S. indica and Tre were significant for all studied traits. The results of the mean comparison showed that salinity stress signifcantly decreased germination percentage, radical length, hypocotyl length, seedling dry weight, seedling fresh weight and K with increasing Na and phenol content. Meanwhile, S. indica and Tre treatments promoted germination parameter. The results indicate that the negative effects of NaCl on tomato plants were alleviated after S. indica inoculation and trehalose. Also in pot experiment, the results of analysis of variance showed that all studied traits were affected by salinity, S. indica, trehalose, and their interaction. The results of the mean comparison indicated that salinity stress signifcantly decreased biomass, relative water content, photosynthetic pigments and K with increasing catalase, electrolyte leakage, hydrogen peroxide, Na and proline content. Meanwhile, S. indica and Tre treatments promoted biomass and enhanced proline, K, catalase and photosynthetic pigments content under salt stress.
 
Conclusion: This study is novel in showing the salt adaptation of tomato plants by use of S. indica and Tre, which can be a promising strategy for improving salt tolerance.
 

Keywords

Main Subjects

References

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Journal of Agricultural Science and Sustainable Production
Volume 35, Issue 2
July 2025
Pages 317-335

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