Effect of Application Type of Plant Growth Promoting Bacteria on Growth, Yield and Nutrient Uptake of French lettuce under Hydroponic Culture

Document Type : Research Paper

Authors

1 Department of Horticultural Science, Faculty of Agriculture, University of Tabriz

2 Department of Horticulture, Faculty of Agriculture, University of Tabriz

3 University of tabriz

4 Department Of Horticulture Science, faculty of Agriculture, University of Tabriz

Abstract

 
Abstract
Background and Objectives: Nowadays, in order to achieve the maximum production, it is inevitable to use the soil microbial potential especially plant growth promoting bacteria (PGPB), Plant growth-promoting bacteria can enhance nutrient uptake efficiency, strengthen the root system, and increase the plant's resistance to environmental stresses, thereby contributing to improved growth and product quality. The aim of the present experiment was to investigate the use of several plant growth-promoting bacteria and their application method effects on growth, yield, and nutrient absorption in lettuce in hydroponic system.
 
Material and Methods: For this purpose, to investigate the effect of PGPB with half the concentration of Hoagland's nutrient solution on the growth characteristics and nutrient uptake of French lettuce under hydroponic conditions, a factorial experiment was carried out in the form of a randomized complete block design with three replications. The first factor was using PGPB including (Pseudomonas sp. C16-2O, Enterobacter cloacae S16-3, Ensifer sp. 3MDP-1) and the second factor was type of inoculation and how to use these bacteria; in the form of foliar spraying, adding to the planting bed (root inoculation) and using them simultaneously.
 
Results: The results showed that the use of Ensifer sp. 3MDP-1 bacteria in the form of addition to the substrate along with foliar spraying had the highest plant fresh weight (169.05 g) and the highest number of leaves (27.66). Foliar spraying of bacteria along with adding to the bed and also using as a foliar spray had a better result on the leaf surface area (respectively 2852.15 and 2781.2 cm2) than the method of adding to the bed. The simultaneous application of Pseudomonas sp. C16-2O bacterium, at the aerial and root parts, it showed the greatest effect on the lettuce root fresh (25.96 g) and dry weight (3.46 g). The treatment of adding Pseudomonas sp. C16-2O bacterium to the substrate with leaf spraying resulted in the production of total chlorophyll of lettuce leaves (35.8) and antioxidant property of lettuce leaves (0.16 μg/mg), while all traits in the control was less than inoculated treatments.
 
Conclusion: The results of this experiment, in addition to proving the growth-stimulating effects of bacteria in inoculated plants compared to the control without inoculation, showed that the simultaneous use of bacteria in the form of leaf spraying and application in the substrate (root inoculation) intensifies the positive effects of bacteria.     
 

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References

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Journal of Agricultural Science and Sustainable Production
Volume 35, Issue 2
July 2025
Pages 187-202

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