Comparison of the role of Steinernema carpocapsae and Bacillus thurengiensis in Control of Tuta absoluta under Laboratory and Greenhouse conditions

Document Type : Research Paper

Authors

1 Member of science

2 member of sciense

3 member of science

Abstract

Background and objective: The tomato leafminer, Tuta absoluta is a devastating key pest of tomato, and its damage may reach up to 100%. Here, the potential of the infective juveniles (IJs) of the entomopathogenic nematode Steinernema carpocapsae species on different larval stadia of T. absoluta was studied under laboratory conditions and the ability of most effective nematode concentration when applied in the greenhouse conditions.
 
Material and Methods: In the in vitro, the six concentrations of third-infective juveniles S. carpocapsae (0,125, 200, 355, 632 and 1124 IJs/ml) were tested against the three larval instars, L2, L3 and L4 of T. absoluta. In the greenhouse test, three treatments included; S. carpocapsae (1124 IJs/ml), Bacillus thuringiensis (0.25%, Belthirul 32% WP, Probelte Co.) selected from among the four tested Bt options and control in a completely randomized design with six replicates were conducted. 
 
 
Results: In the Invitro, forty-eight hours after infection, the entomopathogenic nematode was able to infect all instars of the pest. With increasing density of S. feltiae, the pest larva mortality also increased with a significant difference at 1% probability level. The percentage mortality of L2, L3 and L4 at the highest concentration of nematodes (1124 IJs) were 43.5, 78.8 and 93.2%, respectively. In the Invivo,​​ at the lowest concentration of nematodes (125 IJs) were 16.8, 26.7, and 30.3%.The percentage mortality of different T. absoluta larval stages by S. carpocapsae and B. thuringiensis treatments were 77.5%±2.9  and 40%±13.7   with a significant difference at 1 % probability level.
 
Conclusion: The results indicate the potential of S. carpocapsae as a native entomopathogenic nematode could be used in an integrated management measure in controlling of the T. absoluta and Compared to Bt, it played a more effective role in reducing the pest population.
 
 

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Main Subjects

References

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Journal of Agricultural Science and Sustainable Production
Volume 35, Issue 2
July 2025
Pages 337-348

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