The effect of inoculation with biofertilizers on the quantitative and qualitative characteristics of the medicinal plant Hyssopus officinalis at different levels of water deficit stress

Document Type : Research Paper

Authors

1 Department of Agronomy and Plant Breeding, Tabriz Branch, Islamic Azad University, Tabriz, Iran

2 Department of Agronomy and Agroecology, Urmia Branch, Islamic Azad University, Urmia, Iran

3 Dept., of Agronomy and Plant Breeding, Tabriz Branch, Islamic Azad University, Tabriz, Tabriz, Iran.

4 Horticulture Crop Science Research Department, West Azarbaijan Agricultural and Natural Resources Research and Education Center, (AREEO) ,Urmia, Iran.

10.22034/saps.2024.62497.3251

Abstract

Background and Objectives: In arid and semi-arid regions, employing innovative and sustainable solutions to enhance nutrient uptake is crucial. One such approach is the utilization of microorganisms.This research investigated the effect of mycorrhizae and growth-stimulating bacteria on hyssop's quantitative and qualitative characteristics under different irrigation levels.
 
Materials and Methods: The experiment was conducted as a split plot based on a randomized complete block design with three replications at the West Azarbaijan province research centre (Saatlo station) over two crop years. The irrigation levels, including normal moisture conditions (7-day interval), mild stress (10-day interval), and severe stress (14-day interval), were assigned to the main plots, and inoculation with mycorrhiza (My), Azotobacter (Az), Pseudomonas (Pse), Az + Ps, My+Az+Pse and non-inoculation treatment (control) were assigned to sub-plots. In this experiment, the quantitative and qualitative characteristics of the plant were measured.
 
Results: The highest plant height, total fresh weight, and leaf nitrogen content were assigned to the treatment with Pseudomonas (Pse) under normal irrigation conditions. The maximum essential oil percentage and essential oil yield were related to Psetreatment under mild stress conditions. In all biofertiliser treatments, plant height, total fresh weight, chlorophyll a and b, carotenoid content, nitrogen, phosphorus, and potassium content of leaves decreased with increasing water stress. In contrast, proline content and ion leakage increased. Inoculation with My+Az+Pse under mild and severe stress conditions increased plant height, shoot weight, chlorophyll a content, carotenoid, proline, nitrogen, phosphorus, and essential oil yield. This treatment also reduced the amount of ion leakage compared to control.
 
Conclusion: The separate application of each of the Pse, My, and Az treatments under mild stress conditions resulted in obtaining the maximum essential oil yield, and the combined application of My+Az+Pse adjusted water stress on the essential oil yield under severe water deficit conditions.
 

Keywords

Main Subjects

References

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Journal of Agricultural Science and Sustainable Production
Volume 36, Issue 1
2026
Pages 75-88

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