The Efficiency of Enterobacter cloacae Inocula on Growth Properties,, Oil Yield and Type Fatty Acids of Rapeseed (Brassica napus L.)

Document Type : Research Paper

Authors

Abstract

Background & Objective: Biofertilizers play major role in sustainable agriculture. Increasing the longevity and survival of the bacteria in the production of inoculants by using different materials, is notable to increase the effectiveness of biofertilizers. The aim of present study was to investigate the efficiency of liquid inocula of Enterobacter cloacae S16-3 on growth rate and percentage of rapeseed seed oil (Brassica napus L.) cultivar hayola 308.
 
Materials & Methods: The experiment was carried based on completely randomized design (CRD) with 12 treatments (including 9 liquid inoculants; F1-F9, one negative control treatment without adding any bacteria and NPK, and two positive controls e.g. 100NPK and 70NPK) and three replications, in the greenhouse of Agricultural Faculty of University of Tabriz in a sterilized sandy loam soil in 2018 . Nine liquid inoculants of S16-3 were prepared using different amounts of materials including glycerol, polyethylene glycol (PEG), trehalose, carboxymethylcellulose (CMC), Arabic gum (AG), polyvinyl pyrrolidone (PVP), glucose and starch, in different combinations.
 
Results: The results showed that in the presence of E. cloacaebacteria (in the form of nine inoculants), 100NPK and 70NPK treatments, the number of silique per plant, number of seed per plant, leaf chlorophyll index, plant height, stem diameter, root volume per plant, fresh and dry weight of shoot, root and seed, seed yield per plant, oil yield per plant, silique weight per plant, total fresh and dry weight, seed oil percentage and fatty acids content increased. The inoculation effect of E. cloacae liquid inocula on 1000 seed weight was not significant. The results percentage of seed oil and fatty acid analysis showed that the highest percentage of seed oil (47.02%) and the highest amount of oleic acid (53.1%) was obtained by F9 (glycerol, glucose, AG, PEG) treatment, and oil quality was affected by bacterial inoculation.
 
Conclusion: According to the results of this experiment F5 (AG, starch, PEG) and F9 inoculants can be recommended to improve on growth properties, oil yield and type fatty acids of rapeseed.

 

Keywords


Abadian H, Latifi N, Kamkar B and Bagheri B. 2008. The effect of late sowing date and plant density on quantitative and qualitative characteristics of canola (RGS-003) in Gorgan. Journal of Agricultural and Natural Resources Sciences. 15 (5): 78-87. (In Persian).
Abdul M and Fayyazul H. 2006. Effects of sulphur on fatty acid accumulation in Brassica cultivars. International Journal of Agricultural and Biological. 5: 588-592.
Adams MW and JE Grafius. 2001. Yield compensation alternative interperation. Crop Science. 11: 33-35.
Ahmad A and Abdin MZ. 2000. Effect of sulphur application on lipid, RNA and fatty acid content in developing seeds of rapeseed (Brassica campestris L.). Plant Sciences. 150: 71-76.
Akbari P, Ghalavand A and Modarres Sanavy SAM. 2010. Effects of different nutrition systems and biofertilizer (PGPR) on phenology period yield and yield components of sunflower (Helianthus annuus L.). Journal of Crop Production. 2 (3): 119-134. (In Persian).
Alipour ZT and Sobhanipour A. 2012. The Effect of Thiobacillus and Pseudomonas fluorescens inoculation on maize growth and fe uptake. Annals of Biological Research. 3: 1661-1666.
Alizadeh Kh, FeiziAsl V and Eskandari M. 2003. Optimal levels for some characteristics in Brassica oilseed crops in the cold drylands of Iran. Brassica (An International Journal of Brassicas). 5 (3,4): 48-52.
Asadpour SH and Fayaz Moghadam A. 2007. Effects of different planting date and nitrogen levels on corn forage yield and relevant characteristics to quality (VAR.SC704). Journal of Agricultural Science. 17 (1): 39-49. (In Persian).
Asghar HN, Zahir ZA, Arshad M and Khaliq A. 2002. Relationship between in vitro production of auxins by rhizobacteria and their groth-promoting activities in Brassica juncea L. Biology and Fertility of Soils. 35: 231-237.
Asghar HN, Zahir ZA, Arshad M and Khaliq A. 2004. Relationship between invitro production of auxins by rhizobacteria and their growth promoting activities in Brassica juncea L. Biology and Fertility of Soils. 35: 231-237.
Ashfaq Anjum M, Zahir ZA, Ashraf M and Arshad M. 2011. Isolation and screening of rhizobia for auxin biosynthesis and growth promotion of mungbean (Vigna radiata L.) seedlings under axenic conditions. Soil Environment. 30 (1): 18-26.
Azadmard Damirchi S. 2012. Food chemistry and decomposition. Publication of amidi. (In Persian).
Azarmi F,  Malakouti MJ,  Khavazi K and  Saghafi K. 2015. Effect of simultaneous application of Pseudomonas fluorescens and phosphate fertilizers on yield and uptake of phosphorous and micronutrients in canola. Journal of Soil Biology. 3(1): 21-30. (In Persian).
Azizi M, Soltani A and Khavarikhorasani S. 1999. Rapeseed, Physiology, Agriculture, Breeding and Biotechnology (Translation). Publication of University Mashhad. (In Persian).
Barlog, P and Grzebisz  W. 2004. Effect of timing and nitrogen fertilizer application on winter oilseed rape (Brassica nupus L.). I. growth dynamics and seed yield. Journal of Agronomy and Crop Science. 190: 305-310.
Bashan Y, de-Bashan LE, Prabhu SR and Hernandez JP. 2014. Advances in plant growth promoting bacterial inoculant technology: formulations and practical perspectives. Plant and Soil, 378:1–33.
Chegeni Z, Zolfaghari M, Sedighi Dehkordi F and Mahmoodi Sourestani M. 2018. The Effect of Mycorrhizal fungi, PGPRs and chemical fertilizer on yield and essential oil content of dill (Anethum graveolens L.) seed. Journal of Agricultural Science and Sustainable Production. 28(4): 93-104. (In Persian).
Ehteshami SMR, Kashani M and Yousefirad M. 2016. Effect of seed inoculation with Pseudomonas and Azotobacter bacteria on quantitative and qualitative yield of two sesame cultivars (Sesamum indicum L.). Iranian Journal of Seed Science and Research. 3(3):47-57. (In Persian)
Flagella Z, Rotunnon T, Tarantino E, Di-Caterina R and Decaro A. 2002. Changes in seed yield and oil fatty acid composition of high oleic sunflower (Helianthus annus L.) hybrids in relation to sowing date and water regime. European Journal of Agronomy. 17: 3. 221-230.
Gan Y, Angadi SV, Cutforth H, Potts D, Angadi VV and McDonald CL. 2004. Canola and mustard response to short periods of temperature and water stress at different developmental stages. Canadian Journal of Plant Science. 84: 697-704.
Ghadamkhani A, Enayatizamir N and Norouzi Masir M. 2018. Effect of plant growth promoting bacteria on soil available iron and its uptake by wheat. Journal of Agricultural Science and Sustainable Production. 28(2): 53-64. (In Persian).
Ghasemi Piranlo F, Sarikhani MR and Najafi NA. 2019. Study the survival of Enterobacter cloacae bacteria in several solid carriers and effect of prepared inoculants on ggermination and growth of wheat. Journal of Agricultural Science and Sustainable Production. 29 (3): 167-180. (In Persian).
Ghasemi Piranlo F. 2018. The efficiency of several solid and liquid carriers to increase the survival of Enterobacter cloacae. MSc. Faculty of Agriculture, University of Tabriz.
Han HS, Supanjani K and Lee D. 2004. Effect of coinoculation with phosphate and pottasum solublizing bacteria on mineral uptake and growth of pepper and cucumber. Agronomy Journal. 24: 169- 176.
Hasanzadeh A and Javadi H. 2016. Study on the effects of inoculation with biofirtilizers (Azotobacter and Azospirillum) and nitrogen application on oil, yield and yield components of spring canola in west Azerbaijan. Journal of Crop Production and Processing. 5 (18): 39-50. (In Persian).
Hassanzadeh E, Mazaheri D, Chaichi MR and Khavazi K. 2008. Efficiency of phosphorus solubilizing bacteria and phosphorus chemical fertilizer on yield and yield components of barley cultivar (Karoon Dar Kavir). Pajouhesh and Sazandegi. 77: 111-118.
Herrmann L and Lesueur D. 2013. Challenges of formulation and quality of biofertilizers for successful inoculation. Applied Microbiology and Biotechnology. 97:8859–8873.
Jambhulkar PP and Sharma P. 2014. Development of bioformulation and delivery system of Pseudomonasfluorescens against bacterial leaf blight of rice Xanthomonas oryzae cv. Oryzae. Journal of Environmental Biology. 35 (5): 843-849.
Kadivar SH, Ghavami M, Gharachorloo M and Delkhosh B. 2010. Chemical evaluation of oil extracted from different varieties of colza. Journal of Food Technology and Nutrition. 7 (2): 19-29.
Kazemi Oskuei B, Bandehagh A, Sarikhani MR and Komatsu S. 2018. Protein profiles underlying the effect of plant growth promoting rhizobacteria on Canola under osmotic stress. Journal of Plant Growth Regulation. 37 (2): 560-574.
Kazemi S, Galeshi SA, Ghanbari A and Kianoush GhA. 2005. Effects of sowing date and rhizobium inoculation on yield and its components in soybean. Journal of Agricultural Sciences and Natural Resources. 12(4): 80-87. (In Persian).
Khajehpour MR. 2005. Industrial Crops. Jahade-e-Daneshghahi Isfahan Press. (In Persian).
Khaliq A. 2004. Irrigation and nitrogen management effects on productivity of hybrid sunflower (Helianthus annuus L.). Ph.D. thesis, Department of Agronomy, University of Agriculture. Faisalabad, Pakistan.
Khatamain OS, Modares Sanavy SAM, Ghanati F and Mostavafi M. 2011. Evaluation of yield, its components and some morphological traits of sixteen rapeseed oil cultivars in Arak region. Journal of  Agricultural Science and Sustainable Production. 21(3): 147-161. (In Persian).
Khayavi M, Baghaei N and Nosrati S. 2017. Oilseed rape production guidelines.  Ministry of Agriculture Jihad. Zanjan, Iran. (In Persian).
Kinny AJ. 1997. Genetic engineering of oil seeds for desired traits. 19: 149-166.
Li H, LeiP, Pang  X, Li  S, Xu H, Xu Z  and  FengX. 2017. Enhanced tolerance to salt stress in canola (Brassica napus L.) seedlings inoculated with the halotolerant Enterobacter cloacae HSNJ4. Applied Soil Ecology. 119: 26-34.
Mashhadi Asghari S and Aliasgharzadeh N. 2005. Comparison of five carriers of Sinorrhizobium meliloti to produce alfalfa inoculant. Journal Water and Soil Sciences. 8(4): 63-75. (In Persian).  
Masood M, Shamsi IH and Khan N. 2003. Impact of row spacing and fertilizer levels (Diammonium phosphate) on yield and yield components of canola. Asian Journal of Plant Sciences. 2 (6): 454-456.
Mohamadvarzi R, Habibi D, Vazan S and Pazoki AR. 2010. Effect of plants growth promoting rhizobacteria and Nitrogen fertilizer on yield and yield components of sunflower. 5th National Conference on New Ideas in Agriculture.Esfahan, Iran. Pp. 1-11. (In Persian).
Mohammadi T, Azizi MH and Taslimi A. 2007. Relation of fatty acids composition with stability of sunflower and canola oil blends. Journal of Food Science and Technology. 4: 67–76.
Moller C and Schierholt A. 2002. Genetic variation of palmitate and oil content in a winter oilseed rape doubled haploid population segregating for oleat content. Crop Science. 42: 379-384.
Moradi Sh and Sarikhani MR. 2016. Comparison of dissolution of phosphate from sources of phosphate rock and Tricalcium phosphate by some phosphate solubilizing bacteria. Second National Congress for the Development of Agricultural Science and Natural Resources. Gorgan. Iran. Pp. 1-6. (In Persian).
Naseri F. 1991. Oil Seeds (translation). Publication of Astan Qods Razavi, Mashhad.                                                                     
Nelda R, Paz R, Masson L, Ortiz J, Gonzalez K, Tapia K and Dobaganes C, 2007. Effect of a-tocopherol, a-tocotrienol and rosa mosqueta shell extract on the performance of antioxidant-stripped canola oil (Brassica Sp.) at high temperature. Food Chem. 104: 383-389.
Nosheen A, Bano A and Ullah F. 2013. The role of plant growh promoting rhizobacteria on oil yield and biodiesel production of Canola (Brassica napus L.). Energy Sources. 35: 1574-1581.
Nouriyani H. 2015. Effect of different nitrogen levels on yield, yield components and some quality characteristics of two cultivars of rapeseed (Brassica napus L.). Journal of Crop Production and Processing. 5(16): 233-241. (In Persian).      
Ostadi Jaafari A, Rezvani Moghaddam P and Ghorbani R. 2012. Study of Beneficial Levels and Effect of Azotobacter spp. and Azospirillium spp.Iranian Journal of Field Crops Research. 10 (2): 277-283.
Prajapati K and Modi HA. 2016. Growth Promoting Effect of Potassium Solubilizing Enterobacter hormaechei (KSB-8) on Cucumber (Cucumis sativus) under Hydroponic Conditions. International Journal of Advanced Research in Biological Sciences.3 (5): 168-173.
Ramesh A, Sharma SK, Sharma MP, Yadav N and Joshi OP. 2014. Plant growth promoting traits in Enterobacter cloacae subsp. dissolvens MDSR9 isolated from soybean rhizosphere and its impact on growth and nutrition of soybean and wheat upon inoculation. Agricultural. Research. 31: 53–66.
Roesti D, Gaur R, Johri BN, Imfeld G, Sharma S, Kawaljeet K and Aragno M. 2006. Plant growth stage, fertilizer management and bioinoculation of Arbuscular mycorrhizal fungi and plant growth promoting rhizobacteria affect the rhizobacterial community structure in rainfed wheat fields. Soil Biology and Biochemistry. 38. 1111–1120.
Sadeghi S, Heidari Gh and Sohrabi Y. 2015. Effect of biological fertilizer and fertilization management on some growth indices of two maize varieties. Journal of Agricultural Science and Sustainable Production. 25(3): 43-60. (In Persian).
Saeed KS, Ahmed SA, Hassan IA and Ahmed PH. 2015. Effect of biofertilizer and chemical fertilizer on growth and yield in cucumber Cucumis sativus in green house condition. Pakistan Journal of Biological Sciences. 18: 129–134.
Sajedi NA, Madani H and Mirzakhani M. 2011. Evaluation of biochemical fertilizers on agronomical traits and oil percentage in sunflower. New Finding in Agriculture. 5(4): 377-387. (In Persian).
Sarikhani MR, Khoshru B, Greiner R. 2019. Isolation and identifcation of temperature tolerant phosphate solubilizing bacteria as a potential microbial fertilizer. World Journal of Microbiology and Biotechnology 35:126
Sarikhani MR, Oustan S, Ebrahimi M and Aliasgharzad N. 2018. Isolation and identification of potassium releasing bacteria in soil and assessment of their ability to release potassium for plants. European Journal of Soil Science. 9p.
Sarikhani MR, Ali Asgharzad N and Khooshroo B. 2018. Effectiveness study of phosphate solubilizing bacteria in the formulation of phosphatic microbial fertilizers on Corn. Iranian Journal of Soil and Water Research. 49(1): 71-81. (In Persian).
Savic TB, Kricka T, Voca N, Jurisic V and Matin A. 2009. Effect of storage temperature on rapeseed quality. Agricultural Conspectus Science. 74 (3): 143-147.
Seyed Sharifi R. 2016. Aplication of biofertilizers and zink increases yield, nodulation and unsaturatedfatty acids of soybean. Zemdirbyste- Agriculture. 103 (3): 251-258.
Seyed sharif R, Seyyedi MN and Zaefizadeh M. 2012. Influence of various levels of nitrogen fertilizer on grain yield and nitrogen use efficiency in canola cultivars. Journal of Crops Improvement. 13(2): 51-60. (In Persian).
Shahid M, Hameed S, Imran A, Ali S and Elsas JD. 2012. Root colonization and growth promotion of sunflower (Helianthus annuus L.) by phosphate solubilizing Enterobacter sp. Fs-11. World J Microbiol Biotechnol. 28:2749–2758.
Shankar M, Ponraj P, Ilakkiam D and Gunasekaran P. 2011. Root colonization of a rice growth promoting strain of Enterobacter cloacae. Journal of Basic Microbiology. 51: 523–530.
Shaukat K, Afrasayad S and Hasman S. 2006. Growth responses of Helianthus annuus to plant growth promoting rhizobacteria used as a biofertilizer. Journal of Agricultural Research. 1: 573-581.
Shehata MM and EL-Khawas SA. 2003. Effect of two biofertilizers on growth parameters, yield characters, nitrogenous components, nucleic acids content, minerals, oil content, protein profiles and DNA banding pattern of sunflower yield. Pakistan Journal of Biological Sciences. 6 (14): 1257-1268.
Steer BT and Seiler GI. 1990. Changes in fatty acid composition of sunflower (Helianthus annuus L.) seeds in response to time of nitrogen application, supply rates and defoliation. Journal of the Science of Food and Agriculture. 51: 11-26.
Tahir M, Sajjad Mirza M, Zaheer A, Rocha Dimitrov M, Smidt H and Hameed S. 2013. Isolation and identification of phosphate solubilizer Azospirillum, Bacillus and Enterobacter strains by 16S rRNA sequence analysis and their effect on growth of wheat (Triticum aestivum L.). Australian Journal of Crop Science. 7 (9):1284-1292.
Tayo TO and Morgan DG. 1979. Factor influencing flower and pod development in oilseed rape. Journal of Agricultural Science - Cambridge Core. 92: 363-373.
Yasari E and Patwardhan AM. 2007. Effects of (Azotobacter and Azosprillium) Inoculants and Chemical Fertilizers on Growth and Productivity of Canola (Brassica napus L.). Asian Journal of Plant Sciences. 6 (1): 77-82.