Changes in Chlorophyll Content and Fluorescence and Total Soluble Sugars Rapeseed Cultivars under Osmotic Stress

Document Type : Research Paper

Authors

Abstract

     In order to evaluate the effect of osmotic stress on total soluble sugars, chlorophyll fluorescence and chlorophyll content (SPAD) in the seedling stage of rapeseed, an experiment was conducted as split plot based on randomized complete block design with four replications. The levels of osmotic stress (control, -3.5 and -7 bar) as main factor and 12 rapeseed cultivars as sub factor were used. Results showed that osmotic stress decreased total soluble sugars, Fo, Fv and Fm, but increased SPAD and Fv/Fm. It seemed that increasing of Chlorophyll content caused decreasing of light repression and increasing of Fv/Fm. The Fv/Fm can be used as a physiological index for selection osmotic stress tolerant cultivars. In osmotic stress condition (-7 bar) The Licord, Zarfam and Orient cultivars had the highest Fv/Fm. Also, the Licord cultivar had high biological yield in stress and non stress condition. The amount of stress Tolerant Index (STI) in this cultivar was higher than others. In factor analysis, the four first factors explained 92.04 present of trait variance. The first factor had positive and high coefficients for biological yield and STI and the second factor had positive and high coefficients for chlorophyll fluorescence. Grouping of cultivars using tow first factor showed that Licord cultivar was osmotic stress tolerant and separated from other cultivars.

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