Evaluation of biofilm formation ability by biofilm growth promoting bacteria at the root surface of wheat (Triticum aestivum L.) and their effect on its yield

Document Type : Research Paper

Authors

1 University of Maragheh

2 University of Tabriz

Abstract

Background & Objective: Rhizospheric growth-promoting-bacteria isolated from wild plants are introduced as biofilm forming PGPR. Due to their mode of action they behave as winner in plant root colonization process in the soil. Because of such advantage they are good candidates for the efficient inoculum production industries.
Materials and Methods: This experiment was performed under controlled conditions by inoculation of wheat seeds with nine biofilm forming growth promoting bacteria in a completely randomized design with three replications, and the growth characteristics of wheat were investigated under non-stress conditions.
Results: The bacterial biofilm forming on wheat root was verified under confocal microscopic imaging. From the structural point of view, the diverse biofilms were detected among the bacteria. Total dry weight, stem dry weight, root dry weight, total shoot fresh weight, stem fresh weight and spike fresh weight, second leaf chlorophyll content, and wheat height were affected by the bacterial inoculation. Bacillus toyonensis 18-2 and Bacillus zhangzhouensis 23-3 were able to increase the total dry weight by 15% and 12%, respectively; compared to control treatment.
Conclusion: The biofilm formation by PGPR isolated from non-crops on the surface of wheat root showed that these bacteria have the ability to utilize wheat root secretions and settle in its rhizosphere. Due to the positive effect of the bacteria on wheat growth, the rhizosphere of non-crop plants can be considered as a suitable source for the isolation of new PGPR. The above mentioned two bacteria can be used as a potential candidate for the future experiments.

Keywords


Basharat H, Zaman M, Farooq S, Fatima S, Sayyed RZ, Baba A, Sheikh TA, Reddy MS., El Enshasy H, Suriani NL. 2021. Bacterial Plant Biostimulants: A Sustainable Way towards Improving Growth, Productivity, and Health of Crops. Sustainability 13(5): 28-56.
Bechtaoui N, Raklami A, Benidire L, Tahiri A, Gottfert M, Oufdou K. 2020. Effects of PGPR Co-inoculation on Growth, Phosphorus Nutrition and Phosphatase/Phytase Activities of Faba Bean under Different Phosphorus Availability Conditions. Polish Journal of Environmental Studies, 29(2):1557-1565.
Bolwerk A, Lagopodi AL, Wijfjes AH, Lamers GE, and Chin AWTF, Lugtenberg BJ, and Bloemberg GV. 2003. Interactions in the tomato rhizosphere of two Pseudomonas biocontrol strains with the phytopathogenic fungus Fusarium oxysporum f. sp. radicis-lycopersici. Molecular Plant-Microbe Interactions Journal, 16:983–993.
Chen L, Liu Y, Wu G, Njeri KV, Q. Shen, N. Zhang, R. Zhang. 2016. Induced maize salt tolerance by rhizosphere inoculation of Bacillus amyloliquefaciens SQR9. Physiologia Plantarum, 158(1):34-44
Danhorn T, Fuqua C. 2017. Biofilm formation by plant-associated bacteria. Annual Review of Microbiology, 61:401-22.
Deng S, Caddell DF, Xu G, Dahlen L, Washington L, Yang J. 2021. Genome wide association study reveals plant loci controlling heritability of the rhizosphere microbiome. ISME J 2021. In press.
Ekinci M, Turan M, Yildirim E, Güne A, Kotan R, and Dursun A. 2014. Effect of plant growth promoting rhizobacteria on growth, nutrient, organic acid, amino acid and hormone content of Cauliflower (Brassica oleracea L. var. botrytis) transplants. Acta Scientiarum Polonorum, Hortorum Cultus, 13(6): 71-85
Gholami, H., Ghasemi, J. and Sookhtanlo, M. 2018. Extension of Bio-fertilizers for Environment Conservation: Challenges and Mechanisems. The 13th National Conference on Watershed Management Science and Engineering of Iran and the 3rd National Conference on Conservation of Natural Resources and Environment. Ardebil, Iran Pp: 1-7.
Grote U. 2014. Can we improve global food security? A socio-economic and political perspective. Food Security, 6(2): 187-200.
Cherif-Silini H, Silini A, Yahiaoui B, Ouzari I and Boudabous A. 2016. Phylogenetic and plant-growth-promoting characteristics of Bacillus isolated from the wheat rhizosphere. Annals of Microbiology 66: 1087–1097.
Igiehon NO and Babalola OO. 2017. Biofertilizers and sustainable agriculture: exploring arbuscular mycorrhizal fungi. Applied Microbiology and Biotechnology, 101(12): 4871-4881
Khosravi, H. 2021. Evaluation of plant growth-promoting properties of native azotobacter isolates and the effect of their inoculation on growth of forage maize under salinity stress. Journal of Soil Biology, 9(2): 171-188. (In Persian)
Mathivanan S, Chidambaram ALA, Sundramoorthy P, Baskaran L, and Kalaikandhan R. 2014. Effect of combined inoculations of plant growth promoting rhizobacteria (PGPR) on the growth and yield of groundnut (Arachis hypogaea L.). International Journal of Current Microbiology and Applied Sciences, 3(8): 1010-1020
Pandey A, and Palni LMS. 1997. Bacillus species: The dominant bacteria of the rhizosphere of established tea. Microbiological Research, 152: 359-365.
Porter SS, Sachs JL. 2020. Agriculture and the disruption of plant–microbial symbiosis. Trends Ecology Evolution, 35(5):426–39.
Rostamikia Y, Tabari Kouchaksaraei M, Asgharzadeh A, and Rahmani A. 2016. The effect of plant growth-promoting rhizobacteria on growth and physiological characteristics of corylus avellana Seedlings. Ecopersia, 4 (3): 1471-1479.
Saleh-Lakha S and Glick BR. 2006.  Plant growth-promoting bacteria. In: van Elsas JD, Jansson JK, Trevors JT (Eds) Modern soil microbiology. CRC/Thomson Publishing, Boca Raton, FL/UK, Pp 503–520.
Seneviratne G, Kecskes ML and Kennedy IR .2008. Biofilmed biofertilisers: novel inoculants for efficient nutrient use in plants. Pp: 126–130. In: Kennedy IR, Choudhury ATMA, Kecskes ML and Rose MT (eds). Efficient nutrient use in rice production in Vietnam achieved using inoculants biofertilisers. Proceedings of a project (SMCN/2002/073) workshop, Hanoi, Vietnam.
Timmusk S, Abd El-DaimI A, Copolovici, TanilasT, Kännaste A, Behers L, Nevo E, Seisenbaeva G, Stenström E, Niinemets Ü. 2014. Drought tolerance of wheat improved by rhizosphere bacteria from harsh environments: enhanced biomass production and reduced emissions of stress volatiles, PLOS One 9(5): e96086.
Wang DC, Jiang CH, Zhang LN, Chen L, Zhang XY and Guo JH. 2019. Biofilms positively contribute to Bacillus amyloliquefaciens 54-induced drought tolerance in tomato plants. International Journal of Molecular Sciences, 20(24):6271.
Xun W, Shao J, Shen Q, Zhang R, 2021. Rhizosphere microbiome: Functional compensatory assembly for plant fitness. Computational and Structural Biotechnology Journal 19: 5487–5493.
Yeoh YK, Dennis PG, Paungfoo-Lonhienne C, Weber L, Brackin R and Ragan MA. 2017. Evolutionary conservation of a core root microbiome across plant phyla along a tropical soil chronosequence. Nature communications, 8 (215): 1-9.
Zhou Y, Gao X. 2019. Characterization of biofilm formed by phenanthrene-degrading bacteria on rice root surfaces for reduction of PAH contamination in rice. International Journal of Environmental Research and Public Health, 16(11):2002.