Impact of Foliar Spraying with Different Substances on the Growth and Establishment of Milk Thistle (Silybum marianum) Seeds under Varied Watering Conditions

Document Type : Research Paper

Authors

1 university of urmia

2 University of urmia

10.22034/saps.2024.60197.3166

Abstract

Objective: This study aimed to comprehensively assess the impact of various compounds applied through foliar spraying on the yield and structure of Milk Thistle (Silybum marianum) seeds under diverse irrigation conditions.
Materials and Methods: The investigation was carried out in two distinct settings field and laboratory during the 2017-2018 agricultural season. The research took place at a farm in the province of West Azarbaijan, specifically in Naqhadeh city. Additionally, a research laboratory at the Faculty of Agriculture, Urmia University, was utilized for specific experimental aspects. The field experiment employed a split-plot design, specifically a randomized complete block design with three replications. Irrigation, the first aspect under examination, involved three levels: full irrigation, supplementary irrigation before flowering, and no irrigation. Foliar spraying treatments included salicylic acid, zinc, amino acid, and a control group treated with pure water.
Results: The study revealed that plants attained maximum height with full irrigation. Irrigation significantly influenced capital number, overall capital count, harvest index, stomata count, relative water content, weight of 1000 seeds, seedling longitudinal index, radicle length, shoot length, as well as the activity of catalase enzyme and superoxide dismutase. Both irrigation and foliar application independently affected these factors, and their interaction also played a significant role.
Conclusion: The findings demonstrated that the combined use of foliar spraying and irrigation, as well as their interactive effect, resulted in a significant improvement in the examined characteristics compared to the control group.

Keywords

Main Subjects

References

Aebi H.1984. [13] Catalase in vitro. Methods in enzymology, 105, pp.121-126. DOI: 10.1016/S0076-6879(84)05016-3
Afkari A. 2020. Effect of acid salicylic on germination indices, kernel vigor and activity of some antioxidant enzymes of sunflower (Helianthus annuus L.) cultivar Armavireski under drought stress. Iranian journal of seed science and research‚ 7(1): 79-91. (In Persian). DOI: 10.22124/jms.2020.4342
Ahmadpour R, Rostami M and Hosseinzadeh S R. 2019. Influence of compost tea on seedling growth and germination indices of bean (Phaseolus vulgaris L.) in order to moderated negative effects caused by drought stress. Journal of Plant Research (Iranian Journal of Biology), 32(3): pp.511-523. (In Persian) DOR: 20.1001.1.23832592.1398.32.3.3.0
Ahmadvand G, Dehghan Banadaki M, Alimoradi J, Goudarzi S and Ardalani S. 2018. Reaction of Germination and Seedling Growth of Redroot Pigweed (Amaranthus retroflexus) to Salinity and Drought Stress. Iranian Journal of Seed Research‚ 4(2): 23-35. (In Persian). DOI: 10.29252/yujs.4.2.23
Ahmadvand G, Dehghan, M, Goudarzi S and Fallahi A. 2016. Germination and seedling growth of hory cress (Cardaria draba L.) and madwort (Alyssum hirsutum L.) weeds to salinity and drought stress. Seed Ecophysiology Journal, 1(2): pp.153-164. (In Persian).  DOI: 10.22077/sej.2016.427
Alazem M, Kim K H and Lin N S. 2019. Effects of abscisic acid and salicylic acid on gene expression in the antiviral RNA silencing pathway in Arabidopsis. International Journal of Molecular Sciences, 20(10): 25-38. DOI: 10.3390/ijms20102538
Ali B S‚ ElSayed‚ A I Doheem M A‚ Eita A M A‚ and Omar A A. 2020. Effect of Milk Thistle (Silybum marianum (L.) Gaertn) Seed Extract on Bacterial Activities and Growth of Human Liver Cancer Cells. Journal of Biotechnology Research‚ 6(5): pp.27-33. DOI: 10.32861/jbr.65.27.33
Arampatzis D A‚ Karkanis A C‚ and Tsiropoulos N G. 2019. Silymarin content and antioxidant activity of seeds of wild Silybum marianum populations growing in Greece. Annals of Applied Biology‚ 174(1): 61-73.  DOI: 10.1111/aab.12470
Babaei S and Afkari A. 2023. Effect of superabsorbent polymer application on some agrophysiological traits and yield of chickpea (Cicer arietinum L.) plant under water deficit stress in horand region. Bi-Quarterly Journal of Plant Production‚ 13(1): pp 31-44. (In Persian). DOI: 10.2./jpps.2023.705324
Choudhary R C, Kumaraswamy R, Kumari S, Sharma S, Pal A, Raliya R, Biswas P, Saharan  V and Saharan, V. 2019. Zinc encapsulated chitosan nanoparticle to promote maize crop yield. International Journal of Biological Macromolecules, 127: 126-135. DOI: 10.1016/j.ijbiomac.2018.12.274
Dar Zafar M, Khan F A, Aziz M A, Malik M A, Masood A and Wani F R. 2023. Physiological modulation of plants by salicylic acid under drought stress-A review. SKUAST Journal of Research, 25(1): pp.1-12.  DOI: 10.5958/2349-297X.2023.00001.6
De Marco A‚ Luongo G‚ Di Marino C‚ De Tommaso G‚ Di Fabio G and Zarrelli A. 2020. Silymarin from Silybum marianum by Naviglios extractor: a new and very efficient approach. Natural product research‚ pp.1-7. DOI: 10.1080/14786419.2019.1687474
Ebdal-Aal A. 2018. Effect of foliar spray with lithovit and amino acids on growth, bioconstituents, anatomical and yield features of soybean plant. Annals of Agricultural Science, Moshtohor, 56(4th ICBAA). pp.187-202.DOI: 10.21608/assjm.2018.65137
Fallah Huseini H‚ Hemati A and Alavian S. 2004. A review of herbal medicine: Silybum marianum. Journal of Medicinal Plants‚ 3 (11) :14-24. (In Persian). DOR: 20.1001.1.2717204.2004.3.11.2.2
 Farahdost R and Jafari A A. 2020. Effect of Drought Stress on Seed Germination Characteristics in the Populations of Four Native Species of Onobrychis sp. In Iran. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research‚ 27(2): pp.309-318. (In Persian) DOI:10.22092/ijrfpbgr.2019.114792.1268
Fazeli Kazemi S F‚ Nezami A‚ Parsa M and Kafi A. 2015. Evaluation of components of germination and seedling growth of sesame ecotypes (Sesamum indicum L.) under salt stress conditions. Environmental stresses in crop sciences‚ 7(2):217-232. (In Persian). DOR: 10.22077/escs.2015.180
Ghamari Zare A‚ Ghasemzadeh Daghigh A and Jafari A. 2005. The Effect of irrigation on Quantitative and qualitative yield in ten genotypes of Festuca arundinacea in summer cut. Genetic research and improvement of pasture and forest plants of Iran‚ 12(4):345-363. (In Persian). DOI:  10.22092/ijrfpbgr.2005.115396
Ghiyasi M, Rezaee Danesh Y, Amirnia R, Najafi S, Mulet J M and Porcel R. 2023. Foliar Applications of ZnO and Its Nanoparticles Increase Safflower (Carthamus tinctorius L.) Growth and Yield under Water Stress. Agronomy, 13(1): p.192. DOI: 10.3390/agronomy13010192
Giannopolitis C N and Ries SK. 1977. Superoxide dismutases: I. Occurrence in higher plants. Plant pHysiology‚ 59(2): pp.309-314. DOI: 10.1104/pp.59.2.309
Hafez E, Omara A E D and Ahmed A. 2019. The Coupling Effects of Plant Growth Promoting Rhizobacteria and Salicylic Acid on Physiological Modifications, Yield Traits, and Productivity of Wheat under Water Deficient Conditions. Agronomy, 9(9): 524. DOI: 10.3390/agronomy9090524
Hegab R H, Fawy H A and Habib A A. 2020. Evaluates effect of amino acids, humic acid and antioxidants as foliar application on the biochemical content and productivity of wheat under North Sinai soils conditions. American Journal of Agriculture and Forestry, 8: pp.167-174. DOI: 10.11648/j.ajaf.20200804.19
Heidari M‚ Taleei A and Abbasi A. 2018. Study of the effects of drought stress on seed germination factors and activity of antioxidant enzymes in Bean genotypes (Phaseolus vulgaris L.). Sciences of agricultural plants of Iran‚ 49(1): 11-27. (In Persian). DOI: 10.22059/ijfcs.2017.209308.654148
Jamshidi A‚ Ahmadi A‚ Karimi M and Motesharezadeh B. 2020. Evaluation of some growth and physiological responses of Chia (Salvia hispanica L.) to various moisture regimes. Sciences of agricultural plants of Iran‚ 50(4): 99-110. (In Persian). DOI: 10.22059/ijfcs.2018.260605.654491
Januszkiewicz R, Kulczycki G and Samoraj M. 2023. Foliar Fertilization of Crop Plants in Polish Agriculture. Agriculture, 13(9): p.1715. DOI: 10.3390/agriculture13091715
Kafi M, Nezami A H M AD, Hoseyni H and Masoumi A, 2005. Physiological effects of drought stress by polyethylene glycol on germination of lentil (Lens culinaris Medik.) genotypes. Iranian Journal of Field Crops Research, 3(1): pp.69-80. (In Persian). DOI: 10.22067/gsc.v3i1.1293
Kazempour A, Sharghi Y, Modarres Sanavi S A M and Zahedi H. 2023. Effect of amino acid foliar application on morphophysiological characteristics and thyme essential oil under different irrigation regimes. Journal of Plant Process and Function, 12(53): pp.71-90. (In Persian). DOR:  20.1001.1.23222727.1402.12.53.5.3
Laleh S‚ Jami Al-Ahmadi M and Parsa S. 2017. Effect of Different Levels of Organic and Chemical Fertilizers on Yield, Harvest Index and Extract Percentage of Hemp (Cannabis sativa L.). Agricultural research in Iran‚ 15(4):823-837. (In Persian). DOI: 10.22067/gsc.v15i4.54427
Lefevere H‚ Bauters L and Gheysen G. 2020. Salicylic acid biosynthesis in plants. Frontiers in plant science‚ 11: p.338. DOI: 10.3389/fpls.2020.00338
Maguire, J.D., 1962. Speed of germination-aid in selection and evaluation for seedling emergence and vigor. Crop science. No 2‚ pp: 176-177. DOI: 10.2135/cropsci1962.0011183X000200020033x
Marques T L, Moraes L M B and Rocha F R. 2020. Systematic evaluation of sample preparation for fractionation of phytohormone salicylic acid in fresh leaves. Talanta, 208, 120352. DOI: 10.1016/j.talanta.2019.120352
Mazaraei A‚ Sirousmehr A R and Babaei Z. 2017. Effect of mycorrhizal fungi on some morphological & physiological charactristics of Milk thistle (Silybum marianum L.) Gaertn under drought stress. ranian Journal of Medicinal and Aromatic Plants‚ 33(4):620-635. (In Persian). DOI: 10.22092/ijmapr.2017.107860.1877
Memari M, Dadshi M, Sheikh F and Faghani E. 2020. Seed Yield Response of Faba Bean (Vicia faba L.) Genotypes to Drought Stress. Seed and plant journal‚ 36(2): pp 241-254. (In Persian). DOI: 10.22092/sppi.2020.123208
Mirshekari M‚ Einali A and Valizadeh J. 2017. Physiological and biochemical responses of Hibiscus sabdariffa to drought stress in the presence of salicylic acid. Iranian Journal of Plant Biology‚ 9(32):21-38. (In Persian). DOI: 10.22108/ijpb.2017.100883.1002
Mohammadi M‚ Pouryousef M‚ Tavakoli A and Mohseni Fard E. 2020. Study the effect of Epibrassinolide application on the activity of some antioxidant enzymes, nitrate reductase, and photosynthetic pigments of common bean under drought stress. Iranian Journal of Pulses Research‚ 11(2):76-94. (In Persian). DOI: 10.22067/ijpr.v11i2.76131
Mohtashami F‚ Pouryousef M‚ Andalibi B and Shekari F. 2015. Effects of seed priming and foliar application of salicylic acid on yield and essence of fennel (Foeniculum vulgare Mill.) under drought stress condition. Iranian Journal of Medicinal and Aromatic Plants‚ 31(5):841-852. (In Persian). DOI: 10.22092/ijmapr.2015.103620
Nezami A, Khazaie H R, Barjasteh A R and Zand E. 2017. Effect of drought stress and maternal growth conditions on germination and seedling growth of wild oat (Avena ludoviciana). Seed Science and Technology, 6(1). DOI: (In Persian). DOI: 10.22034/ijsst.2017.113942
 Panahyan Kivi M. 2019. Effects of zinc sulfate foliar spray on yield, yield components and essential oil of coriander (Coriandrum sativum L.) under drought stress. Research of medicinal and aromatic plants of Iran‚ 35(2): 309-322. (In Persian). DOI: 10.22092/ijmapr.2019.122718.2362
Pashang D, Weisany W and Ghajar F G K. 2023. Effect of foliar application of auxin on morphophysiological and biochemical traits of safflower cultivars (spring and autumn) under drought stress. Journal of Environmental Stresses in Crop Sciences‚16(1): pp20-33. (In Persian). DOI: 10.22077/escs.2021.4271.2001
Rady M‚ Saker M and Matter M. 2018. In vitro culture, transformation and genetic fidelity of Milk Thistle. Journal of Genetic Engineering and Biotechnology‚ 16(2): 563-572.  DOI: 10.1016/j.jgeb.2018.02.007
Rajab Nasab Aghamahali M and Karaptian Zh. 2013. The effect of water stress in the flowering stage on plant height and seed protein (Carthamus tinctorius L.) different numbers of Gelrang. Plant environmental physiology‚ 8(31): 37-46. (In Persian). DOI: 10.22077/escs.2020.2526.1665
Rashidi S‚ Abbasdokht H‚ Gholami A and Afshari R T. 2019. Effect of hormone priming and deterioration of seed germination characteristics and antioxidant enzymes activity in corn cultivars seed (Zea mays L.). Iranian Journal of Seed Science and Research‚ 6(2). (In Persian). DOI: 10.22124/jms.2019.3602
Rezaei- chiyaneh E‚ Rahimi A‚ Sheykh F and Mohayeji M. 2018. Responses of some agronomical characteristics of faba bean (Vicia Faba L.) to biofertilizers under water deficit stress. Journal of crop production‚ 10(4):107-120. (In Persian). DOI: 10.22069/ejcp.2018.12238.1943
Ritchie S W‚ Nguyen H T and Holaday A S. 1990. Leaf water content and gas‐exchange parameters of two wheat genotypes differing in drought resistance. Crop science‚ 30(1): pp.105-111.DOI:10.2135/cropsci1990.0011183X003000010025x
Saed-Moucheshi  A and Safari H, 2023. Investigation of regulatory elements related to superoxide dismutase enzyme genes in wheat. Journal Cereal Biotechnology and Biochemistry, (1): pp 65-77. DOI: 10.22126/cbb.2023.8692.1034
Salarpour F and Farahbakhsh H. 2016. Effects of salicylic acid on some physiological traits, yield and yield components of fennel (Foeniculum vulgare Mill L.) under drought stress. Research of medicinal and aromatic plants of Iran‚ 32(2):216-230. (In Persian). DOI: 10.22092/ijmapr.2016.106557
Shabanzade Sh and Gholvi M. 2011. The effect of foliar application of micronutrient elements and irrigation frequency on agricultural characteristics and yield of black seed. Environmental stresses in crops sciences. 4(1):1-9. (In Persian). DOI: 10.22077/escs.2011.94
Shamsaie M‚ Sodaei zadeh H and Tajamolian M. 2017. Evaluation of the effects of drought stress of mother plants on some seed germination indices of Satureja hortensis. Desert management‚ 4(8):27-35. (In Persian). DOI: 10.22034/jdmal.2017.24659
Sharafizadeh M. 2017. Effect of salicylic acid and drought stress on germination and activity of antioxidant enzymes of barely. Iranian Journal of Seed Science and Technology, 6(2): pp.161-169. (In Persian). DOI: 10.22034/ijsst.2018.116567
Shekari F‚ Soltaniband V‚ Javanmard A and Abbasi A. 2016. The impact of drought stress at different stages of development on water relations, stomatal density and quality changes of rapeseed (Brassica napus L.). Iran Agricultural Research‚ 34(2): pp.81-90. (In Persian). DOI: 10.22099/iar.2016.3452
Song X, Guo H, Liu Y, Wan F, Zhang J and Chang X. 2020. Effects of salicylic acid and sucrose on pigment content in Pistacia chinensis leaves. Scientia Horticulturae, 259, 108783. DOI: 10.1016/j.scienta.2019.108783
Tabatabayi A. 2014. The effect of pretreatment of barley seeds with salicylic acid on seedling growth, proline content and antioxidant enzyme activity under drought stress conditions. Journal of Crops Improvement‚ 16(2):475-486. (In Persian). DOI: 10.22059/jci.2014.53056
Teixeira W F, Fagan E B, Soares L H, Umburanas R C, Reichardt K and Neto D D. 2017. Foliar and seed application of amino acids affects the antioxidant metabolism of the soybean crop. Frontiers in plant science, 8: p.327. DOI: 10.3389/fpls.2017.00327
Umair Hassan M‚ Aamer M‚ Umer Chattha M‚ Haiying T‚ Shahzad B, Barbanti L‚ Nawaz M‚ Rasheed A‚ Afzal A‚ Liu Y and Guoqin H. 2020. The critical role of zinc in plants facing the drought stress. Agriculture‚10(9): pp.396. DOI: 10.3390/agriculture10090396
Wang H and Clarke J M. 1993. Genotypic, intraplant, and environmental variation in stomatal frequency and size in wheat. Canadian Journal of Plant Science‚ 73(3): pp.671-678. DOI: 10.4141/cjps93-08
Yazdani Biyoki R‚ Rezvani Moghadam P‚ Khazayi H R‚ Ghorbani R and Astarayi A R. 2010. The effects of salinity and drought stress on the germination characteristics of Maritigal seeds (Silybum marianum L.). Iranian journal of field crops research‚ 8(1):12-19. (In Persian). DOI: 10.22067/gsc.v8i1.7385
JOURNAL OF AGRICULTURAL SCIENCE AND SUSTAINABLE PRODUCTION
Volume 34, Issue 3
October 2024
Pages 327-349

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