Evaluation of Essential oil Components of Some Medicinal plant Mentha piperita L. Ecotypes in Different Harvesting and Drying Methods

Document Type : Research Paper

Authors

1 Department of Horticultural science, Faculty of Agriculture and Natural resources, University of Mohaghegh Ardabili, Ardabil, Iran.

2 Department of Horticultural science, Faculty of Agriculture and Natural resources, University of Mohaghegh Ardabili, Ardabil, Iran

3 Department of Horticultural science, Faculty of Agriculture and Natural resources, University of Lorestan, Khorramabad, Lorestan, Iran

10.22034/saps.2025.60046.3159

Abstract

Background and Objective: Preserving plant biodiversity is important for creating structural diversity and main compounds in the future for the sustainable development of human civilization. Peppermint (Mentha piperita L.), with numerous medicinal properties is one of the most important plants from Lamiaceae family. These plants produce a major and diverse group of secondary metabolites. Main purpose of this research is evaluation of essential oil components of some Mentha piperita L. ecotypes in different harvesting and drying methods.
 
Materials and Methods: In this research, the diversity of essential oil compounds in different methods of harvesting (50% flowering- 100% flowering) and drying in different (oven- shade) accesion of peppermint plants was investigated. This experiment was conducted in University of Mohaghegh Ardabili climatic condition.in 2022, based on randomized complete block design with three replications. Their essential oils were extracted by using of Clevenger apparatus and were analyzed by GC-MS.
 
Results: Analysis of variance showed that there were significant differences among studied essential oil compounds traits. In total, 25 compounds were identified, and the largest amount of compounds was found in the Zanjan accession in the harvesting and drying method 50% flowering-shade and the highest number of compounds was observed in the Noorabad accession. The most important components of peppermint essential oil were: Limonene (0.23-29.45%), Menthol (0.30-30.16%), Cadinol (0.21.17.40%), Pulegone (0.35-13.72), Ledol (0.20-3.86%), Propazulen (0.26-5.28%) and Terpinol (0.21-2.85%). Maximum correlation was shown between Naphthalene and Aromadendrene (0.80). Cluster analysis, classified the treatments into four major groups. Factor analysis indicated that the 9 factors explained 93.19 of the variability among the accessions.
 
Conclusion: The results suggested that Mentha piperita L accessions of Iran had a high genetic diversity that can be used in the breeding programs. The considerable variations were observed among accessions in viewpoint of number, type and amount of essential oil compounds.

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References

Ahmadpour A and Mahdavikia H. 2019. Comparative investigation of antifungal activities of some medicinal plants essential oils on Alternaria alternata fungus as a causal agent of tomato early blight disease. Eco-phytochemical Journal of Medicinal Plants, 7 (2): 1-24. (In Persian). https://civilica.com/doc/922018
Azizi A, Ardalani H & Honermeier B. 2016. Statistical analysis of the associations between phenolic monoterpenes and molecular markers, AFLPs and SAMPLs in the spice plant Oregano. Herba Polonica, 62: 42-56. http://dx.doi.org/10.1515/hepo-2016-0010
Bader A, AlQathama A, Cioni P L, Ceccarini L, Abdelhady M I S, Al-Shareef W, Ascrizzi R and Flamini G. 2022. Essential oil biodiversity of Achillea ligustica All. Obtained from Mainland and Island populations Plants, 11: 1054. https://doi.org/10.3390/plants11081054
Baharmast Z, Kheiry A, Sanikhani M and Soleimani A. 2020. Study and comparison of morphological and phytochemical traits of Mentha pulegium L. in different habitats of Guilan province. Eco-phytochemical Journal of Medicinal Plants, 8 (2): 60-75. (In Persian with English Abstract).http://dx.doi.org/10.61186/jcb.16.49.129
Bouyahya A, Abrini J, Dakka N and Bakri Y. 2019. Essential oils of Origanum compactum increase membrane permeability, disturb cell membrane integrity, and suppress quorum-sensing phenotype in bacteria. Journal of Pharmaceutical Analysis, 9: 301-311. https://doi.org/10.1016/j.jpha.2019.03.001
Fitzgerald M, Heinrich M and Booker A. 2020. Medicinal Plant Analysis: A Historical and Regional Discussion of Emergent Complex Techniques. Frontiers in Pharmacology, 10: 1480. https://doi.org/10.3389/fphar.2019.01480
Isayenkov S V and Maathuis F. J. 2019. Plant salinity stress: many unanswered questions remain. Frontiers in Plant Science, 10: 1-11. https://doi.org/10.3389/fpls.2019.00080
Khadivi-Khub A, Salehi-Arjmand H, Movahedi K and Hadian J. 2015. Molecular and morphological variability of Satureja bachtiarica in Iran. Plant systematics and evolution, 301(1): 77-93. https://doi.org/10.1007/s00606-014-1055-3
Milica A, Mirjana C and Jovana S. 2016. Effect of Weather Conditions, Location and Fertilization on Coriander Fruit Essential Oil Quality. Journal of Essential Oil-Bearing Plants, 19 (6): 1208-1215.
http://dx.doi.org/10.1080/0972060X.2015.1119068
Nejadhabibvash F. 2017. Phytochemical composition of the essential oil of Anthemis wiedemanniana Fisch. and C. A. Mey. (Asteraceae) from Iran during different growth stages. Journal of Essential oil-Bearing Plants, 20(5): 1349- 1359. https://doi.org/10.1080/0972060X.2017.1383191
Nejadhabibvash F and Rezaee M. 2018. Variation in the essential oil of Achillea wilhelmsii C. Koch. and A. millefolium L. apical shoots in different developmental stages in Martyrs Valley, West Azerbaijan province: A Case example. Journal of Plant Biology, 10 (1): 65-72. https://doi.org/10.22108/ijpb.2018.105469.1038
Olatunya A.M and Akintayo E.T. 2017. Evaluation of the effect of drying on the chemical composition and antioxidant activity of the essential oil of peels from three species of citrus group. International Food Research Journal, 24(5): 1991-1997. http://www.ifrj.upm.edu.my
Razzazi N, Jafari A, Khodarahmpour Z and Sadat Sh. 2021. Phytochemical comparison of essential oils in five species of Stachys L. in Khorramabad agronomic conditions. Eco-phytochemical Journal of Medicinal Plants, 9 (3): 1-15. (In Persian with English Abstract).https://doi.org/10.30495/ejmp.2023.1988348.1732
Rezapour K, Naghdi Badi H, Amini Dehaghi M and Kordenaeej A. 2023. Evaluation of essential oil diversity of different populations of Thymus fallax Fisch. & C.A. Mey. Collected from some of its habitats in Iran. J. Med. Plants; 22 (85): 59-75. (In Persian). http://dx.doi.org/10.61186/jmp.22.85.59
Rita P and Animesh D.K. 2011. An updated overview on peppermint (Mentha piperita L.). International Research Journal of Pharmacy, 2(8):1-15. http://dx.doi.org/10.3390/nu11040739
Sadat N and Ladan Moghadam A.R. 2019. Effect of salicylic acid foliar application on control of NaCl salt salinity on some morphological, physiological traits and growth of peppermint mint (Mentha piperita). Cellular and Molecular Plant Biology Journal, 13(3): 31-43. (In Persian with English Abstract). https://doi.org/10.22092/ijmapr.2021.343340.2810
Sefidkon F, Abdollahi M, Salehi Shanjani P and Tavakoli M. 2021. Variability in essential oil content and composition of Achillea tenuifolia Lam. populations in field conditions. Journal of Agricultural Science and Technology, 23(3): 673-683. http://jast.modares.ac.ir/article-23-35617-en.html
Seif Sahandi M, Naghdi Badi H, Mehrafarin A, Khalighi-Sigaroodi F and Sharifi M. 2019. Changes in Essential Oil Content and Composition of Peppermint (Mentha piperita L.) in Responses to Nitrogen Application. Iranian Journal of Medicinal and Aromatic Plants, 4 (72): 1-18. (In Persian). http://dx.doi.org/10.29252/jmp.4.72.81
Sun Z, Wang H, Wang J, Zhou L and Yang P. 2014. Chemical ‎Composition and Anti-Inflammatory. Cytotoxic and Antioxidant Activities ‎of Essential Oil from Leaves of Mentha piperita Grown inChina. Plos ‎One, 9(12): 1- 15‎‏.‏ https://doi.org/10.1371/journal.pone.0114767
Tutar U, Karaman I, Çelik C, Ataş M and Hepokur C. 2016. Anti-biufilm antimicrobial activity of Mentha pulegium essential oil resistant-multidrug against Acinetobacter baumannii. Tropical Jornal of Pharmaceutica Research, 15(5): 1046-1053. http://dx.doi.org/10.4314/tjpr.v15i5.20
Journal of Agricultural Science and Sustainable Production
Volume 35, Issue 4
2026
Pages 241-253

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