The effect of Beef blood powder and Protein Hydrolyzing Bacteria on the morphophysiological traits of basil (Ocimum basilicum )plants

Document Type : Research Paper

Authors

1 Dept. of Horticulture, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran.

2 gricultural Science and Technology at the University of Mohaghegh Ardabili

3 University of Mohaghegh Ardabili. ardebil. iran

4 Agricultural Science and Technology at the University of Mohaghegh Ardabili. ardebil. iran

10.22034/saps.2026.69687.3416

Abstract

Background and Objective: Due to the negative effects of chemical fertilizer, the need to use healthier solutions for agricultural production seems essential. Therefore, managing industrial slaughterhouse waste as a rich source of nutrients and amino acids has been one of the main concerns of researchers in recent decades. In this regard, a study was conducted to evaluate the growth of basil plant under the conditions of using blood powder from slaughterhouses and the effects of microorganisms on improving the availability of nutrients and amino acids in blood powder.
 
Materials and Methods: This experiment was conducted as a factorial in a completely randomized design with three replications in the greenhouse of Mohaghegh Ardabili University in the winter of 2024 as pot culture. The experimental treatments included blood powder at four levels (0, 2, 4 and 6 g/kg) and the second factor was the inoculation of protein hydrolyzing bacteria and growth stimulants in three treatments (control, Bacillus licheniformis bacteria, Bacillus subtilis bacteria).
 
Results: Blood powder at 6 g/kg caused a 25.9% increase in plant height, an increase (59.85, 65%) in root fresh weight and root dry weight, respectively, compared to the treatment without blood powder. Inoculation with Licheniformis bacteria increased the fresh dry weight of the shoot by (27.97, 35.53%) in the treatment with 6 g/kg blood powder. The highest number of leaves (27.7) and the highest root length (17.95 cm) were obtained at a blood powder concentration of 6 g/kg along with inoculation with bacillus licheniformis bacteria. Also, at a blood powder concentration of 6 g/kg, inoculation with bacillus licheniformis bacteria caused an 18% increase in leaf area compared to the treatment without inoculation with hydrolyzing bacteria. Also, at a concentration of 6 g/kg of blood powder, inoculation with bacillus licheniformis bacteria caused an increase of 23.46, 32.48, 16.83 and 25.71% of chlorophyll, b, a carotenoid and total chlorophyll compared to the absence of inoculation with hydrolyzing bacteria. The highest amount of total phenol (22.41 mg/g) was obtained at a concentration of 6 g/kg of blood powder along with inoculation with bacillus licheniformis bacteria.
Conclusion: According to the results obtained from the research, inoculation with protein hydrolyzing bacteria causes better decomposition of blood powder in the soil. Therefore, it increases the growth of basil plants and improves the physiological indicators of basil plants.
 

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References

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Journal of Agricultural Science and Sustainable Production
Volume 37, Issue 3
October 2027
Pages 249-269

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