The effect of Chemical and Biological Fertilizers on some Morphological Traits and the Advantages of safflower (Carthamus tinctorius) and fenugreek Intercropping

Document Type : Research Paper

Authors

1 Ph.D student of Plant Ecophysiology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran

2 Department of Ecophysiology, Faculty og Agriculture, University of Tabriz

3 Department of Plant Ecophysiology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran

10.22034/saps.2026.67916.3378

Abstract

Background & Objectives: The tendency to produce oilseeds, as well as the demand for oilseed products, is growing worldwide, particularly under organic settings. Intercropping systems, as a kind of sustainable production, can make better use of available resources. This study was carried out to determine the effect of chemical and biological fertilizers on some morphological features and advantage indices of safflower in various intercropping patterns with fenugreek.
 
Materials and Methods: The experiment was carried out as a factorial experiment in a randomized complete block design with three replications at the Faculty of Agriculture's research farm in Tabriz. The experimental treatments consisted of a two-level cropping pattern (pure safflower and 100:50 additive intercropping (safflower to fenugreek)) and a six-level fertilizer treatment. (control, 100% urea recommended by soil test, foliar application of NPK, Barvar 2 phosphate + 100% urea, Barvar 2 phosphate + 50% urea, Barvar 2 phosphate + NPK).
 
Results: According to the results, the most grain were produced in the capital by the treatment combination of Barvar 2 phosphate + NPK in pure cultivation and Barvar 2 phosphate + 100% urea in intercropping (with 61.8 and 64.47 numbers, respectively). The intercropping system resulted in a lower yield of safflower seeds than in pure cultivation.The highest grain yield (332.6 g m-2) and oil yield (85.18 g m-2) were obtained from pure cultivation of this plant in conjunction with the application of 100% urea. When 100% urea was applied to safflower grown in pure conditions, the grain yield rose by 7.9% and the oil yield by 1.5% in comparison to the control. However, the treatment of applying 50% urea along with Barvar 2 phosphate improved the grain yield by 4.1% and the oil output by 8.4% compared to the control. The 100% urea application treatment had the highest land equivalency ratio (2.1), while the NPK foliar application treatment had the lowest index (1.5). Conversely, the Barvar 2 phosphate + 50% urea application treatments produced the best relative yield of safflower (1.37), while the NPK foliar application treatment produced the highest relative yield of fenugreek (0.47).
 
Conclusion: According to the study's findings, the most effective nutritional treatments for boosting safflower's grain and oil yields were 50% urea + Barvar 2 phosphate in intercropping and 100% urea in pure cultivation. As a result, these treatments can be suggested for farmers use. 

Keywords

Main Subjects


Agha Baba Dastjerdi M, Amini Dehaghi M, Bosaghzadeh Z and Shafiee Adib S. 2017. Effect of biofertilizer on yield and quality characteristics of fennel in additive intercropping with prennial alfalfa. Plant Production Technology, 9(1): 125-140. https://doi.org/10.22084/ppt.2017.2208
Ahmadpour Abnvi S, Ramroudi M, Galavi M and Shamsaddin Saied M. 2019. Effect of biological and chemical phosphorus fertilizer on yield and yield components of safflower (Carthamus tinctorius L.) under low irrigation conditions. Journal of Agricultural Science and Sustainable Production, 29(1): 269-284.‏ (In Persian with English Abstract).  
Amini E, Taab A and Radicetti E. 2024. Investigating the quantitative and qualitative yield of bitter vetch (Vicia ervilia) and grass pea (Lathyrus sativus) in intercropping with wheat (Triticum aestivum) under different tillage systems. Iranian Journal of Field Crop Science, 55(3): 197-210. (In Persian with English Abstract). ‏. https://doi.org/10.22059/ijfcs.2024.373410.655067.
Banik P, Midya A, Sarkar BK and Ghose SS. 2006. Wheat and chickpea intercropping systems in an additive experiment: Advantages and weed smothering. European Journal of Agronomy, 24: 325-332. https://doi.org/10.1016/j.eja.2005.10.010
Board JE and Settimi JR. 1986. Photoperiod effect befor and after flowering on branch development in determinate soybean. Agronomy  Journal, 78: 995-1002.
Bybordi A. 2010. Effects of salinity and N on the growth, photosynthesis and N status of canola (Brassica napus L.). Notulae Scientia Biologicae, 2(2): 92-97.
Darzi M, Hadj Seyed Hadi M and Rejali F. 2012. Effects of cattle manure and plant growth promoter bacteria application on some morphological traits and yield in Coriander (Coriandrum sativum L.). Iranian Journal of Medicinal and Aromatic Plants Research, 28(3): 434-446. (In Persian with English Abstract). .  https://doi.org/10.22092/ijmapr.2012.2945
El Mouttaqi A, Mnaouer I, Nilahyane A, Belcaid M, Ibourki M, Lazaar K, Diatta L, Devkota KP, Kouisni L and Hirich A. 2022. How does organic amendment and NPK fertilization improve forage yield of cereals under salinity and arid conditions? Case of Moroccan Sahara. Environmental Sciences Proceedings, 16(1): 51.‏ https://doi.org/10.3390/environsciproc2022016051
El-Sayed MAM. 2013. Effect of interactıon between biofertilizers and saline irrigation water on the productıvıty of safflower (Carthamus tinctorius L) ın northern sinai-egypt. Journal of Soil Sciences and Agricultural Engineering, 4(8): 793-809. https://doi.org/10.21608/jssae.2013.52068
Fan L, Zhao HY, Xu M, Zhou L, Guo H, Han J, Wang BR and Guo DA. 2009. Qualitative evaluation and quantitative determination of 10 major active components in Carthamus tinctorius L. by high-performance liquid chromatography coupled with diode array detector. Journal of Chromatography A, 1216(11): 2063-2070. https://doi.org/10.1016/j.chroma.2008.03.046
Feng W, Ge J, Rodríguez ARS, Zhao B, Wang X, Peixoto L, Yang Y, Zeng Z and Zang H. 2024. Oat/soybean strip intercropping benefits crop yield and stability in semi-arid regions: A multi-site and multi-year assessment. Field Crops Research, 318: 109560.‏ https://doi.org/10.1016/j.fcr.2024.109560
Gawinowski M, Enjalbert J, Cournède PH and Flutre T. 2024. Contrasted reaction norms of wheat yield in pure vs mixed stands explained by tillering plasticities and shade avoidance. Field Crops Research, 310: 109368.‏ https://doi.org/10.1101/2022.10.27.514050
Gendy AS, Abdelkader MA, El-Naggar NZ and Elakkad HA. 2018. Effect of intercropping systems and NPK foliar application on productivity and competition indices of black cumin and fenugreek. Current Science International, 7(3): 387-401.‏
Guo L, Li H, Cao X, Cao A and Huang M. 2021. Effect of agricultural subsidies on the use of chemical fertilizer. Journal of Environmental Management, 299: 113621.‏
Habtamu M, Elias E, Argaw M and Feleke G. 2024. Intercropping wheat (Triticum aestivum) with faba bean (Vicia faba) combined with vermicompost and NPS fertilizer application increases crop yields and agronomic efficiency in the humid mid-highlands of Ethiopia. Cogent Food and Agriculture, 10(1): 2324539. https://doi.org/10.1080/23311932.2024.2324539
‏Hamdan YA, Pérez-Vich B, Velasco L and Fernández-Martínez JM. 2009. Inheritance of high oleic acid content in safflower. Euphytica, 168(1): 61-69. https://doi.org/10.1007/s10681-008-9879-y
Hesan M. 2023. Life cycle assessment of an NPK fertilizer production with the focus on principal harmful substances (Master's thesis, University of South-Eastern Norway).‏ 137 Pages.
Irum A, Pasha MFK, Aziz H, Akram M, Rehman WU, Momin A, Naheed R, Mubeen A and Ullah S. 2024. Rhizobacterial inoculation to enhance growth and productivity of fenugreek. Indus Journal of Bioscience Research, 2(02): 1329-1333. https://doi.org/10.70749/ijbr.v2i02.392
Islam MR, Hossain J, Alam MA, Islam MS, Rahman MM, Laing AM and Hossain A. 2024. Growth, productivity, competitive ratio, maize equivalent yield, land equivalent ratio, and profitability of hybrid maize as influenced by relay cropping with mukhikachu (Colocasia esculenta Schott.) during rabi season. Current Applied Science and Technology, e0256415-e0256415.‏ https://doi.org/10.55003/cast.2023.256415
Jahan M, Nassiri-Mahallati M, Amiri MB and Ehyayi HR. 2013. Radiation absorption and use efficiency of sesame as affected by biofertilizers inoculation in a low input cropping system. Industrial Crops and Products, 43: 606-611. https://doi.org/10.1016/j.indcrop.2012.08.012
Kader MA, Main MH and Hoque MS. 2002. Effects of Azotobacter inoculant on the yield nitrogen uptake by wheat. Online Journal of Biological Sciences, 2(4): 259-261. https://doi.org/10.3923/jbs.2002.259.261
Kaleri AA, Sadiq M, Jamali A, Ullah Z, Musawwir A, Islam M, Sadiqa A, Kakar K, Manzoor D and Adil S. 2024. Effects of phosphorus application rate on the growth and yield of wheat (Triticum Aestivum L.). Pak-Euro Journal of Medical and Life Sciences, 7(2): 151-158.‏ https://doi.org/10.31580/pjmls.v7i2.3020
Kubsad VS, Naik VR, Hanumantharaya L and Nekar MM. 2008. Phosphorus management in mungbean-safflower sequence cropping in vertisols under rainfed conditions. In Safflower: unexploited potential and world adaptability. 7th International Safflower Conference, Wagga Wagga, New South Wales, Australia, 3-6 November, 2008. (pp. 1-4). Agri-MC Marketing and Communication.
Ladux FJ, González CV, Trentacoste ER, Searles PS and Rousseaux MC. 2024. Morphological and pigment responses to far-red and photosynthetically active radiation in an olive cultivar suitable for super-high-density orchards. Plants, 13(13): 1822.‏ https://doi.org/10.3390/plants13131822
Li C, Stomph TJ, Makowski D, Li H, Zhang C, Zhang F and van der Werf W. 2023. The productive performance of intercropping. Proceedings of the National Academy of Sciences, 120(2): e2201886120. https://doi.org/10.1073/pnas.2201886120
Li H, Testerink C and Zhang Y. 2021. How roots and shoots communicate through stressful times. Trends in Plant Science, 26(9): 940-952.‏ https://doi.org/10.1016/j.tplants.2021.03.005
Lin Y, Watts DB, Torbert HA and Howe JA. 2020. Influence of nitrogen rate on winter canola production in the southeastern United States. Agronomy Journal, 112: 2978–2987. https://doi.org/10.1002/agj2.20197
Madani H, Malboobi MA, Bakhshkelarestaghi K and Stoklosa A. 2011. Biological and chemical phosphorus fertilizers effect on yield and p accumulation in rapeseed (Brassica napus L.). Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 40(2): 210-214. https://doi.org/10.15835/nbha4026079
Mahfouz SA and Sharaf-Eldin MA. 2007. Effect of mineral vs. biofertilizer on growth, yield, and essential oil content of fennel (Foeniculum vulgare Mill.). International Agrophysics, 21(4): 361-366. https://doi.org/10.1055/s-2007-987419
Mndzebele B, Ncube B, Fessehazion M, Mabhaudhi T and Modi AT. 2023. Assessment of the effects of NPK fertilizer on edible yield and agro-biological parameters in a cowpea–amaranth intercrop. Frontiers in Sustainable Food Systems, 7: 1210984.‏ https://doi.org/10.3389/fsufs.2023.1210984
Mondani F, Yari A, Bagheri A and Chaghazardi HR. 2024. Investigation of radiation absorption and use efficiency in safflower (Carthamus tinctorius) with lentil (Lens culinaris Medik) intercropping under dryland conditions. Journal of Crops Improvement, 26(2): 315-330. https://doi.org/10.22059/jci.2024.361496.2829
Mosalman S, Rezaei-Chiyaneh E, Mahdavikia H, Dolatabadian A and Siddique KH. 2024. Enhancing rainfed safflower yield, oil content, and fatty acid composition through intercropping with chickpea and stress-modifier biostimulants. Frontiers in Agronomy, 6: 1389045. https://doi.org/10.3389/fagro.2024.1389045
Mundel HH and Bergman JW. 2008. Safflower breeding. pp. 423-447, In: Vollmann J and Rajcan I. (Eds.), Oil Crop Breeding, Hand Book of Plant Breeding. Springer, Berlin. https://doi.org/10.1007/978-0-387-77594-4_14
Nagananda GS, Das A, Bhattacharya S and Kalpana T. 2010. In vitro studies on the effects of bio-fertilizers (Azotobacter and Rhizobium) on seed germination and development of Trigonella foenum-graecum L. using a novel glass marble containing liquid medium. International Journal of Botany, 6(4): 394-403. https://doi.org/10.3923/ijb.2010.394.403
Narimani Y, Taleshi K, Khorgami A and Vafaei SH. 2024. Cereal based intercropping systems improve yield advantage and crop yield under superabsorbent application in semiarid conditions. Legume Science, 6(3): e70002.‏ https://doi.org/10.1002/leg3.70002
Ntanos DA and Koutroubas SD. 2002. Dry matter and N accumulation and translocation for Indica and Japonica rice under Mediterranean conditions. Field Crops Research, 74(1): 93-101.  https://doi.org/10.1016/S0378-4290(01)00203-9
Qadeer A, Yaseen M, Naveed M and Shahbaz M. 2024. Effect of urea-phosphate and application methods on maize (Zea mays L.) growth yield and nutrient use efficiency. Applied Ecology and Environmental Research, 22(1).‏
Qasim HM and Hammo YH. 2024. Influence of shading, GA3 and NPK fertilizer on the growth and development of Myrtle Myrtus communis plants. Tikrit Journal for Agricultural Sciences, 24(1): 34-44.‏ https://doi.org/10.25130/tjas.24.1.4
Qin Z, Fang X, Fei JL, Fan W, Chen ZM, Liao WJ, Gu LB, Liu HM, Qin Z, Zhu XL and Wang XD. 2025. Impact of microwave treatment on polyphenols in safflower seeds and oil. Food Chemistry, 143116.‏ https://doi.org/10.1016/j.foodchem.2025.143116
Raei Y, Sayyadi Ahmadabad M, Ghassemi-Golezani K and Ghassemi S. 2020. The effect of biological and chemical nitrogen fertilizers on pinto bean (Phaseolus vulgaris L.) and black mustard (Brasassica nigra L.) intercropping. Journal of Agricultural Science and Sustainable Production, 30(3): 21-40.
Rafiee S, Yadavi A, Dehnavi MM and Balouchi H. 2023. Arbuscular mycorrhizal fungi, phosphate-solubilizing bacteria, and zinc foliar application improve physiological responses in black cumin (Nigella sativa) under drought stress. Agricultural Plant Science, 1-20. https://doi.org/10.21203/rs.3.rs-3194201/v1
Rahimi A, Dovlati B, Amirnia R and Heydarzade S. 2020. Effect of application of mycorrhizal fungus and Azotobacter on physiological characteristics of Trigonella foenum-graecum L. under water stress conditions. Journal of Plant Biological Sciences, 11(4): 1-18.‏ (In Persian with English Abstract). . https://doi.org/10.22108/IJPB.2019.116384.1149
Rahimi Darabad GR, Barmaki M and Seyed Sharifi R. 2015. Evaluation of yield and yield components in potato-safflower intercropping. Agronomy Journal (Pajouhesh and Sazandegi), 108: 114-119. (In Persian with English Abstract). . http://dx.doi.org/10.22092/aj.2016.106730.
Sahoo U, Maitra S, Dey S, Vishnupriya KK, Sairam M and Sagar L. 2023. Unveiling the potential of maize-legume intercropping system for agricultural sustainability: A review. Farming and Management, 8(1): 1-13.‏ https://doi.org/10.31830/2456-8724.2023.FM-124
Said-Al Ahl H, Sarhan A, Dahab M, Abou-Zeid EN, Ali MS and Naguib NY. 2015. Growth and chemical composition of dill affected by nitrogen and bio-fertilizers. International Journal of Life Science and Engineering, 1(2): 75-84.
Setshogela BP. 2024. Influence of harvest time and genotype on seed yield, petal yield and carthamidin and carthamin contents, and mineral nutritional content in safflower (Carthamus tinctorius L.) (Doctoral dissertation, Buan).‏ Botswana University of Agriculture and Natural Resources, 135 Pages.
Seyed Sharifi R, Namvar A and Seyed Sharifi R. 2017. Grain filling and fatty acid composition of safflower fertilized with integrated nitrogen fertilizer and biofertilizers. Pesquisa Agropecuária Brasileira, 52: 236-243. https://doi.org/10.1590/S0100-204X2017000400003
Seyedi M, Hamzei J, Ahmadvand G and Abutalebian MA. 2012. The evaluation of weed suppression and crop production in barley-chickpea intercrops. Journal of Agricultural Science and Sustainable Production. 22(3): 101-115.‏ (In Persian with English Abstract).
Shamohammadi N, Zare M, Ordokhani K, Aref F and Sharafzaeh S. 2024. Grain corn yield under the influence of water deficit stress, biological and chemical fertilizers. Iranian Journal of Field Crop Science, 55(1): 23-36.‏ (In Persian with English Abstract). . https://doi.org/10.22059/IJFCS.2023.352991.654966
Shehata MM and El-Khawas SA. 2003. Effect of two biofertilizers on growth parameters, yield characters, nitrogenous components, nucleic acids content, minerals, oil content, protein profiles and DNA banding pattern of sunflower (Helianthus annus L. cv. Vedock) yield. Pakistan Journal of Biological Sciences, 6(14): 1257-1268. https://doi.org/10.3923/pjbs.2003.1257.1268
Singh A, Singh N, Kalhapure AH, Gupta S, Gupta D, Verma R, Kumar R, Prajapati D and Maurya SK. 2024. Effect of nutrient management on Indian mustard [Brassica juncea (L.)]: A Review. International Journal of Plant and Soil Science, 36(7): 429-438.‏ https://doi.org/10.9734/ijpss/2024/v36i74750
Sun J, Zhao J, Li W, Wu K, Chen L, Yang X, He X and Zheng H. 2025. The effect of nitrogen application on yield stability of maize|| soybean intercropping systems. Chinese Journal of Eco-Agriculture, 33(6): 1138-1145.‏ https://doi.org/10.12357/cjea.20240470 
Sun T, Zhou J, Fu Y, Wu L and Zhang T. 2024. Soil nitrogen availability mediates the positive effects of intercropping on soil organic carbon at global scales. Soil and Tillage Research, 239: 106063.‏
https://doi.org/10.1016/j.still.2024.106063
Takim FO. 2012. Advantages of maize-cowpea ıntercropping over sole cropping through competition ındices. Journal of Agriculture and Biodiversity Research, 1: 53-59.
Tao D, Gao Y, Revillini D, Yan A, Zhou G, Swanson CS, He Q, Ma H, Yu X and Delgado-Baquerizo M. 2024. Root traits regulate the capacity of the rhizosphere to support multiple ecosystem services under intercropping and phosphorus fertilization. Agriculture, Ecosystems and Environment, 374: 109181.‏ https://doi.org/10.1016/j.agee.2024.109181
Tohidinia MA, Mazaheri D, Bagher-Hosseini SM and Madani H. 2013. Effect of biofertilizer Barvar-2 and chemical phosphorus fertilizer application on kernel yield and yield components of maize (Zea mays cv. SC704). Iranian Journal of Crop Sciences, 15(4). 295-307. (In Persian with English Abstract).
Toker P, Canci H, Turhan I, Isci A, Scherzinger M, Kordrostami M and Yol E. 2024. The advantages of intercropping to improve productivity in food and forage production–a review. Plant Production Science, 27(3): 155-169.‏ https://doi.org/10.1080/1343943X.2024.2372878
Wang S, Ruan Y, Du M, Sun W, Zhang Y, Wang Y, Guo J, Shao R, Yang Q and Wang H. 2024. Optimization of phosphate fertilizer application strategies to improve phosphorus availability and utilization in maize. Agronomy Journal, 116(2): 453-464. https://doi.org/10.1002/agj2.21513 ‏
Wang T, Wang K, Wang N, Cui D, Li S, Lu Q and Zuo Y. 2025. From intercropping to monocropping: The effects of Pseudomonas strain to facilitate nutrient efficiency in peanut and soil. Plant Physiology and Biochemistry, 219: 109378.‏ https://doi.org/10.1016/j.plaphy.2024.109378
Wang W, Zhao JH, Li MY, Zhang W, Rehman MMU, Wang BZ, Ullah F, Cheng ZG, Zhu L, Zhang JL and Tao HY. 2024. Yield loss of inferior crop species and its physiological mechanism in a semiarid cereal-legume intercropping system. European Journal of Agronomy, 152: 127032.‏ https://doi.org/10.1016/j.eja.2023.127032
Yang L, Gu C, Huang W, Chang H, Gao Y, Li Y, Dai J, Li X, Hu W, Cao W and Liao X. 2024. Legume and maize intercropping enhances subsequent oilseed rape productivity and stability under reduced nitrogen input. Field Crops Research, 319: 109644.‏ https://doi.org/10.1016/j.fcr.2024.109644
Yari A, Mondani F, Bagheri A and Chaghazardi H. 2024. The effect of intercropping system on radiation use efficiency and growth indices of safflower (Carthamus tinctorius L.) and chickpea (Cicer arietinum L.). Agrotechniques in Industrial Crops, 4(4): 218-228.‏ (In Persian with English Abstract). . https://doi.org/10.22126/ATIC.2024.10889.1153
Zang PH and Sedum PJ. 2005. Interactions among phosphorous, nitrogen and growth in oilseed rape. Canadian Journal of Plant Science, 74(3): 173-181.
Zarei F, Zarei A. 2019. Sustainable Agriculture. Day System. 159 Pages. (In Persian with English Abstract).
Zhou C, Kong D, Li J, Su X, Cai N and Xu Y. 2025. The morphological and physiological responses of Pinus yunnanensis to different levels of shading after decapitation. Industrial Crops and Products, 224: 120374.‏ https://doi.org/10.1016/j.indcrop.2024.120374