Effect of Humic Acid and EDTA on Growth Characteristics, Grain Yield and Yield Components of Oilseed rape (Brassica napus L.) Under Copper Toxicity Stress

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Abstract
A lot of contaminants are entering to the environment, yearly and cause to accumulate of heavy metals in soil such as copper. In order to investigate the morphological traits, grain yield and yield components of oilseed rape (Brassica napus L.) in contaminated soil by copper with application of EDTA and humic acid, a greenhouse experiment was carried out as factorial based on randomized complete block design with three replications. The factors were included application of soil amendment (EDTA and humic acid) at five levels as control (without soil amendment), 29.2 and 292.2 mg.kg-1 EDTA, 1.25 and 2.5 mg.kg-1 humic acid, and soil copper quantity at four levels as control (without copper), 100, 200 and 300 mg.kg-1. The studied traits were height, leaf number and area per plant, branch and pod number per plant, 1000 grain weight, grain yield and shoot dry matter. Increasing of copper concentration in soil decreased morphological traits and yield of oilseed rape. Also positive response of grain yield in polluted soil by using the soil amendment was related to the improved morphological traits and yield components of oilseed rape. Treatment of 292.2 mg.kg-1 of EDTA produced the highest grain yield of oilseed rape. 29.2 mg.kg-1 of EDTA, 1.25 and 2.5 mg.kg-1 of humic acid increased grain yield, but the difference between these treatments with control was insignificant. The effect of EDTA on all traits except 1000 grain weight was more than humic acid. According to critical levels of copper toxicity in relative shoot dry matter and relative grain yield , low concentrations of amendments increased oilseed rape tolerance to copper toxicity compared with high concentrations of amendments.
 
 

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JOURNAL OF AGRICULTURAL SCIENCE AND SUSTAINABLE PRODUCTION
Volume 24, Issue 4.1 - Serial Number 4
February 2015
Pages 103-121

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