Evaluation of Growth and Yield Stability of Wheat by Application of Trichoderma and Enterobacter sp.

Document Type : Research Paper

Authors

Abstract

Abstract
This study was carried out to study the effect of biopriming using Trichoderma hamatum and Enterobacter sp. as plant growth promoting on some morphological characteristics and grain yield (GY) of wheat (Triticum aestivum L. cv. ‘Milan’). A field experiment was conducted in a split factorial arrengment based on randomized complete block design with three replications in Sari (Soteh village) during 2014-2015. Three levels of triple super phosphate (TSP; zero, 50 and 100 kg.ha-1) were applied as the main plot and both fungal (inoculation with Trichoderma hamatum and uninoculated as control) and phosphate solubilizing bacteria (PSB) inoculations (inoculation with Enterobacter sp. and uninoculated as control), were served as the sub-plots. The results indicate a positive effect of both fungal and PSB inoculations on the most studied traits of wheat. Enterobacter sp. increased GY by 19, 7, and 26% when used with zero, 50 and 100 kg.ha-1 of TSP, respectively. In addition, Trichoderma hamatum increased GY and biological yield (BY) by 13 and 10%, respectively, as compared to the control conditions. Enterobacter sp. inoculation along with Trichoderma hamatum enhanced the values of all studied triats such as panicle length (4%), GY (21%) and (10%) as compared to the control. Therefore, these environmental friendly microorganisms can be used as an effective seed inoculants to improve grain yield of wheat and to decrease over application of P fertilizers and also could be useful tools for soil productivity, particularly when were used along with TSP as compared to the application of TSP alone.
 

Keywords

References

Abd-El-Khair H, Khalifa RKM and Haggag KHE, 2010. Effect of Trichoderma species on damping off diseases incidence, some plant enzymes activity and nutritional status of bean plants. Journal of American Science, 6(9): 486-497.‏
Afzal A and Bano A, 2008. Rhizobium and phosphate solubilizing bacteria improve the yield and phosphorus uptake in wheat (Triticum aestivum). International Journal of Agriculture and Biology, 10: 85-88.‏
Ahangar L, Babaeizad V, Ranjbar GA, Najafi Zarrini H and Biabani A, 2015. Expression profile of defense-related genes in susceptible and resistant wheat cultivars in response to powdery mildew infection. Modern Genetics Journal, 10(1): 33-46. (In Persian). 
Ahmadi J, Khatibi M, Amirshekari H and Amini Dehagi M, 2011. Evaluation of the effective morpho-physiological indices on the yield of spring wheat (Triticum aestivum L.) using multivariate statistical methods. Journal of Agronomy Science, 4(4): 55-66. (In Persian). 
Arriagada C, Aranda E, Sampedro I, Garcia-Romera I and Ocampo JA, 2009. Contribution of the saprobic fungi Trametes versicolor and Trichoderma harzianum and the arbuscular mycorrhizal fungi Glomus deserticola and G. claroideum to arsenic tolerance of Eucalyptus globulus.Bioresource Technology, 100: 6250-6257.‏
Badar R and Qureshi SA, 2012(a). Comparative effect of Trichoderma hamatum and host-specific Rhizobium species on growth of Vigna mungo. Journal of Applied Pharmaceutical Science, 2(04): 128-132.‏
Badar R and Qureshi SA, 2012(b). Use of Trichoderma Hamatum alone and in combination with rhizobial isolates as biofertilzer for improving the growth and strength of sunflower. Journal of Basic and Applied Scientific Research, 2(6): 6307-6314.‏ 
Bae H, Sicher RC, Kim MS, Kim SH, Strem MD, Melnick RL and Bailey BA, 2009. The beneficial endophyte Trichoderma hamatum isolate DIS 219b promotes growth and delays the onset of the drought response in Theobroma cacao. Journal of Experimental Botany, 60(11): 3279-3295.‏
Bakhshandeh E, Rahimian H, Pirdashti H and Nematzadeh GHA, 2014. Phosphate solubilization potentail and modeling of stress tolerace of rhizobacteria from rice paddy soil in northen Iran. World Journal of Microbiology and Biotechnology, 30: 2437-2447.
Bakhshandeh E, Rahimian H, Pirdashti H and Nematzadeh GHA, 2015. Evaluation of phosphate‐solubilizing bacteria on the growth and grain yield of rice (Oryza sativa L.) cropped in northern Iran. Journal of Applied Microbiology, 119: 1371-1382.
Bennett AJ and Whipps JM, 2008. Dual application of beneficial micro-organisms to seed during drum priming. Applied Soil Ecology, 38: 83-89.
Chacón MR, Rodríguez-Galán O, Benítez T, Sousa S, Rey M, Llobell A and Delgado-Jarana J, 2007. Microscopic and transcriptome analyses of early colonization of tomato roots by Trichoderma harzianum. International Microbiology, 10: 19-27.‏ 
Chandra Nayaka S, Niranjana SR, Uday Shankar AC, Niranjan Raj S, Reddy MS, Prakash HS and Mortensen CN, 2010. Seed biopriming with novel strain of Trichoderma harzianum for the control of toxigenic Fusarium verticillioides and fumonisins in maize. Archives of Phytopathology and Plant Protection, 43: 264-282.‏ 
Cherati A, 2015. Wheat Nutrition Management. Mazandaran Agricultural and Natural Resources Research Center, Agriculture Education, Extension and Research Organization, Ministry of Jehad-e-Agriculture, Pp: 1-3. (In Persian). 
Doni F, Isahak A, Zain CRCM and Yusoff WMW, 2014. Physiological and growth response of rice plants (Oryza sativa L.) to Trichoderma spp. inoculants. AMB Express-a Springer Open Journal, 4: 2-7.
Ghaderi MG, Zeinali Khanghah H, Hosseinzadeh AH, Taleei AR and Naghavi MR, 2009. Evaluation of relationships between grain yield, yield components and the other characteristics associated with grain yield in bread wheat using multivariate statistical analysis. Iranian Journal of Field Crop Research, 7(2): 573-582. (In Persian).
Hashem A, Abd_Allah EF, Alqarawi AA, Al Huqail AA and Egamberdieva D, 2014. Alleviation of abiotic salt stress in Ochradenus baccatus (Del.) by Trichoderma hamatum (Bonord.) Bainier. Journal of Plant Interactions, 9(1): 857-868.‏
Hassan TU and Bano A, 2015. Role of carrier-based biofertilizer in reclamation of saline soil and wheat growth. Archives of Agronomy and Soil Science, 61: 1719-1731.  
Hermosa R, Viterbo A, Chet I and Monte E, 2012. Plant-beneficial effects of Trichoderma and of its genes.Microbiology,158: 17-25.‏
Hossain MB and Sattar MA, 2014. Effect of inorganic phosphorus fertilizer and inoculants on yield and phosphorus use efficiency of wheat. Journal of Environmental Science and Natural Resources, 7(1): 75-79.‏ 
Javaheri T, Lakzian A, Khorasani R and Taheri P, 2014.Acid and alkaline phosphatase enzyme activities of different strains of soil fungi in the presence of organic compounds of phosphorus (phytic acid and sodium glycerophosphate). Journal of Soil Biology, 2(1): 1-11. (In Persian). 
John RP, Tyagi RD, Prévost D, Brar SK, Pouleur S and Surampalli RY, 2010. Mycoparasitic Trichoderma viride as a biocontrol agent against Fusarium oxysporum f. sp. adzuki and Pythium arrhenomanes and as a growth promoter of soybean.Crop Protection,29: 1452-1459.‏
Kaewchai S, Soytong K and Hyde KD, 2009. Mycofungicides and fungal biofertilizers. Fungal Diversity, 38: 25-50. 
Kızılkaya R, 2008. Yield response and nitrogen concentrations of spring wheat (Triticum aestivum) inoculated with Azotobacter chroococcum strains. Ecological Engineering, 33: 150-156.‏
Lavakush Yadav J, Verma JP, Jaiswal DK and Kumar A, 2014. Evaluation of PGPR and different concentration of phosphorus level on plant growth, yield and nutrient content of rice (Oryza sativa L.).Ecological Engineering,62: 123-128.‏ 
Mahdinezhad N, Omidi M, Jalal Kamali MR, Naghavi MR and Fakheri B, 2015. Effect of salinity stress on some agronomic characteristics, grain yield and its components in Seri/Babax recombinant inbred lines wheat. Iranian Journal of Filed Crop Science, 46(1): 37-48. (In Persian). 
Mirshekari B, Hokmalipour S, Sharifi RS, Farahvash F and Ebadi-Khazine-Gadim A, 2012. Effect of seed biopriming with plant growth promoting rhizobacteria (PGPR) on yield and dry matter accumulation of spring barley (Hordeum vulgare L.) at various levels of nitrogen and phosphorus fertilizers.Journal of Food Agriculture and Environment, 10(3&4): 314-320.‏ 
Mishra DJ, Rajvir S, Mishra UK and Kumar SS, 2013. Role of bio-fertilizer in organic agriculture: a review.Research Journal of Recent Sciences,2: 39-41. 
Mohammadi H, Ahmadi A, Moradi F, Abbasi AR, Poustini K, Joudi M and Fatehi F, 2011. Evaluation of critical traits for improving wheat yield under drought stress. Iranian Journal of Filed Crop Science, 42(2): 373-385. (In Persian). 
Mohammadi K, Ghalavand A, Aghaalikhani M, Heidari G and Sohrabi Y, 2011. Introducing a sustainable soil fertility system for chickpea (Cicer arietinum L.).African Journal of Biotechnology, 10(32): 6011-6020.‏
Mohammadi Kashka F, Pirdashti H, Yaghoubian Y and Bahari Saravi SH, 2015. Effect of Trichoderma virens and Piriformospora indica coexistence with phosphate solubilizing bacteria (Enterobacter sp.) on growth of pepper (Capsicum annuum L.) plant. 2th National Conference of Planning, Protecting and Conservation, Environmental Protection, Sustainable Development. 15 February, Tehran, Iran. Pp: 10. (In Persian).
Naseby DC, Pascual JA and Lynch JM, 2000. Effect of biocontrol strains of Trichoderma on plant growth, Pythium ultimum populations, soil microbial communities and soil enzyme activities. Journal of Applied Microbiology, 88: 161-169.‏ 
Raj SN, Shetty NP and Shetty HS, 2004. Seed bio-priming with Pseudomonas fluorescens isolates enhances growth of pearl millet plants and induces resistance against downy mildew. International Journal of Pest Management, 50(1): 41-48.‏ 
Rawat R and Tewari L, 2011. Effect of abiotic stress on phosphate solubilization by biocontrol fungus Trichoderma sp.. Current Microbiology, 62: 1521-1526.‏ 
Rudresh DL, Shivaprakash MK and Prasad RD, 2005. Tricalcium phosphate solubilizing abilities of Trichoderma spp. in relation to P uptake and growth and yield parameters of chickpea (Cicer arietinum L.). Canadian Journal of Microbiology, 51: 217-222.‏
Sallam Nashwa MA, Abo-Elyousr KAM and Hassan MAE, 2008. Evaluation of Trichoderma species as biocontrol agent for damping-off and wilt diseases of Phaseolus vulgaris L. and efficacy of suggested formula. Egyption Journal of Phytopathology,36(1-2): 81-93.‏
Sengupta C, Bhosale A and Malusare S, 2015. Effect of plant growth promoting rhizobacteria on seed germination and seedling development of Zea mays. International Journal of Research in Advent Technology Special Issue, National Conference on Advances and Chalenges in Green Technology. 13-14 February, Pune, India. Pp: 9.
Shaharoona B, Naveed M, Arshad M and Zahir ZA, 2008. Fertilizer-dependent efficiency of Pseudomonads for improving growth, yield, and nutrient use efficiency of wheat (Triticum aestivum L.). Applied Microbiology and Biotechnology, 79: 147-155.‏ 
Sharifi RS, 2011. Grain yield and physiological growth indices in maize (Zea mays L.) hybrids under seed biopriming with plant growth promoting rhizobacteria (PGPR). Journal of Food Agriculture and Environment, 9(3&4): 393-397.‏ 
Sharma P, Patel AN, Saini MK and Deep S, 2012. Field demonstration of Trichoderma harzianum as a plant growth promoter in wheat (Triticum aestivum L.).Journal of Agricultural Science,4(8): 65-73.‏
Shukla N, Awasthi RP, Rawat L and Kumar J, 2012. Biochemical and physiological responses of rice (Oryza sativa L.) as influenced by Trichoderma harzianum under drought stress.Plant Physiology and Biochemistry,54: 78-88.‏
Singh V, Singh PN, Yadav RL, Awasthi SK, Joshi BB, Singh, RK, Lal RJ and Duttamajumder SK, 2010. Increasing the efficacy of Trichoderma harzianum for nutrient uptake and control of red rot in sugarcane. Journal of Horticulture and Forestry, 2(4): 66-71.‏ 
Taleei A and Bahramnejad B, 2003. A Study of relationship between yield and its components in landrace populations of wheat from western parts of Iran using multivariate analysis. Iranian Journal of Agriculture Science, 34(4): 949-959. (In Persian). 
Verma JP, Yadav J, Tiwari KN and Kumar A, 2013. Effect of indigenous Mesorhizobium spp. and plant growth promoting rhizobacteria on yields and nutrients uptake of chickpea (Cicer arietinum L.) under sustainable agriculture. Ecological Engineering,51: 282-286.‏
Vinale F, Sivasithamparam K, Ghisalberti EL, Marra R, Woo SL and Lorito M, 2008. Trichoderma-plant-pathogen interactions. Soil Biology and Biochemistry, 40: 1-10.
Yadav A, Yadav K and Aggarwal A, 2015. Impact of arbuscular mycorrhizal fungi with Trichoderma viride and Pseudomonas fluorescens on growth, yield and oil content in Helianthus annuus (L.). Journal of Essential Oil Bearing Plants, 18(2): 444-454. 
Zaidi A, Saghir Khan MD and Amil MD, 2003. Interactive effect of rhizotrophic microorganisms on yield and nutrient uptake of chickpea (Cicer arietinum L.).European Journal of Agronomy, 19: 15-21.‏
Zhu F, Qu L, Hong X and Sun X, 2011. Isolation and characterization of a phosphate-solubilizing halophilic bacterium Kushneria sp. YCWA18 from Daqiao Saltern on the coast of Yellow Sea of China. Evidence-Based Complementary and Alternative Medicine, 2011: 1-6.‏
JOURNAL OF AGRICULTURAL SCIENCE AND SUSTAINABLE PRODUCTION
Volume 26, Issue 4
January 2017
Pages 1-15

Files

Share

How to cite

Statistics