Influence of Rhizobium, Pseudomonas and Fungi Mycorrhiza on Some Traits of Red Beans (Phaseolus vulgaris L.) under Drought Stress

Document Type : Research Paper

Authors

Abstract

         To investigate the effect of three types of organic fertilizers on some characteristics of bean (Goli cv.) under water deficit stress an experiment was conducted as split plot as randomized complete block design (RCBD) with three replications in Tabriz during 2014 in Research Farm of Islamic Azad University of Tabriz. Irrigation  levels were: irrigation after 70, 110 and 150 mm evaporation from class A pan and bio-manure in seven levels: Rhizobium (Legominosarrum biovar phaseoli), mycorrhiza (Glomus mosseae), Pseudomonas (fluorescens), Rhizobium+mycorrhiza, Rhizobium+Pseudomonas, Rhizobium+Pseudomonas+mycorrhiza and control were assigned to main plots and sub plots, respectively. The results showed that all traits except the number of stomata on addaxial, abbaxial, cell membrane stability was higher in irrigation after 70 mm evaporation than other irrigation levels. Irrigation after 110 and 150 mm evaporation from pan reduced grain yield as 38.08% and 73.37%, respectively compared to the control. Treatment of Pseudomonas+ Rhizobium + mycorrhizae had higher yield (2401.1 kg.ha-1) compared other treatments except Rhizobium and mycorrhizae. Interaction of water stress and bio-manure was significant for chlorophyll content index, number of stomata on abbaxial and addaxial levels of leaves, cell membrane stability, leaf area index, harvest index, and the average number of seed per plant. Based on this research, bio fertilizer consumption increased all attributes compared to control, and combined use of bio-fertilizers also showed a higher impact than single use of them
 

Keywords


Aboutalebian MA and Khalili M. 2014. Effect of arbuscular mycorrhiza and Bradyrhizobium japonicum on soybean yield and yield components under water stress. Iranian Journal of Field Crop Science, 45)2(:169-181. (In Persian)
Afkari A, Ghasemof N and Yarnia M. 2010. Effect of drought stress and potassium on some of the physiological and morphological traits of sunflower (Helianthus annuus L.). cultivars. 11th Iranian Crop Science Congrees. Tehran. Iran. Pp. 154-156. (In Persian).
Aga-Alikhani M and Tahmacebi Z. 2002. The effect of water stress on yield and yield components of three varieties of beans. 7  th Iranian Crop Science congrees. Tehran. Iran. Pp. 261-264. (In Persian).
Akbari Gh, Ghorchiani M, Alikhani HA, Allahdadi I and Zarei M. 2012. Effect of Biological and Chemical Phosphate Fertilizers on Growth Indices and Grain Yield of Maize under Deficit Irrigation Conditions in Karaj Region Journal Water and Soil Science, 22 (4): 52-67. (In Persian).
Aliasgharzad N, Neyshabouri MR and Salimi G. 2006. Effects of arbuscular mycorrhizal fungi and Bradyrhizobium japonicum on drought stress of soybean. Biologia, Bratislava, 61 (19): 324-328.
Ashraf MY, Azmi AR, Khan AH and Ala SA. 1994. Effect of water stress on total phenols, peroxidase activity and chlorophyll content in wheat. Acta Physiological Plantarum, 16(3): 18 5-191
Askary M and Bayat  L, 2013. Effects Inoculation With Two strain of Rhizobium on Anatomical Indexes of Persian Clover (Trifolium resupinatum L.) Under SO2 Pollution. Journal of Cell & Tissue, 4(3): 261-273. (In Persian).
Bahamin S, Arian-Mehr M, Kardoni F, Mahmodi S and Azar-Pevand H. 2011. Effects of salinity and increasing the growth of bacteria (Pseudomonas fluorescent) on germination and seedling growth characteristics of unflower. The 1st National Conference on Modern Agricultural Sciences & Technologies. Zanjan. Iran. Pp. 195-198.
Banziger M, Edmeades GO, Beck D and Bellon M. 2000. Breeding for Drought and Nitrogen Stress Tolerance in Maize, from Theory to Practice. CIMMYT, Mexico DF.
Behbood M, Golchin A and Besharati H. 2012. The Effects of Phosphorus and Inoculation with Plant Growth Promoting Rhizobacter (PGPR), Pseudomonas Fluorescent, on Yield and Quality of Potato Tuber (Agria Cultivar L.). Journal of Water and Soil, 26 (2): 260-271. (In Persian).
Bromandrad A, Sajedi N, Changizi M and Vasibi M. 2011. effects of Combined chemical fertilizers, and PGPR on the performance of corn. The First National Conference on Strategies for Achieving Sustainable Agriculture. Iran. Khozestan. Pp. 201-204.
Costa-Franca MG, Thi AT, Pimentel C, Pereyra RO, Zuily-Fodil Y and Laffray D. 2000. Differences in growth and water relations among Phaseolus vulgaris cultivars in response to induced drought stress. Environmental and Experimental Botany, 43: 227-237.
Duan X, Neuman DS, Reiber JM, Green CD, Saxton AM and Auge RM. 1996. Mycorrhizal influence on hydraulic and hormonal factors implicated in the control of stomatal conductance during drought. Journal of Experimental Botany, 47:1541-1550.
Ganbari AA, SHakiba MR, CHokan R and Torchi M. 2012. Bean genotypes in normal physiological responses and limited Byatry. PhD. Thesis. In Agronomy. Faculty of Agriculture, University of Tabriz.
Ghorchiani M, Akbari G, Alikhani HA, Allahdadi I and Zarei M. 2001. Effect of Arbuscular Mycorrhizal Fungi and Pseudomonas fluorescence Bacterium on the Ear Traits, Chlorophyll Content and Yield of Zea Maize under Moisture Stress Conditions.  Journal Water and Soil Science, 21 (1): 97-117. (In Persian)
Giri B, Kapoor R and Mukerji KG. 2002. VA mycorrhizal techniques/VAM technology in establishment of plants under salinity stress condition. In: Mukerji, K.G., Manoracheir, C., and Singh, J. (eds.) Techniques in mycorrhizal stueies Kluwer, Dordrecht. Pp. 313-327.
Glick BR. 1995. The enhancement of plant growth by free-living bacteria. Canadian Journal Microbiology, 41: 109-117.
Glick BR, Penrose DM and Jiping LI. 1998. A model for the lowering of plant ethylene concentration by plant growth-promoting bacteria. Journal of Theoretical Biological, 190: 63-68.
Habibi D. 2011.  Effect of plant growth promoting rhizobacteria, foliar application of amino acids and silicic acid on yield and yield co mponents of wheat under drought stress conditions  . Journal of Crop Production Research, 3(1): 71- 87. (In Persian..
Haghighatnia H, Nadian H, Rejali F and Tavakoli AR. 2013. Effect of Two Species of Arbuscular-Mycorrhizal Fungi on Vegetative Growth and Phosphorous Uptake of Mexican Lime Rootstock (Citrus aurantifolia L.) Under Drought Stress Conditions. Seed and Plant Production Journal, 28 (4): 403-417. (In Persian).
Han HS and Lee KD. 2005. Plant growth promoting rhizobacteria effect on antioxidant status, photosynthesis, mineral uptake and growth of lettuce under soil salinity. Research Journal of Agriculture and Biological Science, 1(3): 210-215.
Hu L, Wang Z, Du H and Huang B. 2009. Differential accumulation of dehydrins in response to water stress for hybrid and common Bermuda grass genotypes differing in drought tolerance. Journal of Plant Physiology, 167: 103-109.
 Jabbari F and Khaleghnezhad V. 2014. Consideration of some biofertilizers effect on water relations and gas exchange of chickpea (Cicer arietinum L.) under irrigated and rainfed conditions.  Iranian Journal of Field Crop Science, 45(1): 53-64. (In Persian).
Jalili F, Khavazi K and Asadi-Rahmani H. 2011. Effects of  Fluorescent Pseudomonads with ACC Deaminase Activity on Growth Characteristics of Canola (Brassica napus L.) under Salinity Condition Journal Water and Soil Science, 21 (2): 175-192. (In Persian).
Khalid A, Arshad M and Zahir ZA, 2006. Phytohormones: Microbial production and applications, pp. 207-220. In Uphoff N. (eds.), Biological Approaches to Sustainable Soil Systems. Boca Raton, Florida, USA.
Khandan-Bejandi T, Seyed-Sharifi R, Sedghi M, Asghari-Zakaria R, Namvar A and Jafari-Moghaddam M. 2010. Effect of plant density, rhizobia and microelements on yield and some of morph physiological characteristics of chickpea. European Journal of Crop Production, 3(1): 139-157.
Khan MS, Zaidi A and Wani PA. 2009. Role of phosphate-solubilizing microorganisms in sustainable agriculture: review. Biomed Life Sciences, 5: 551-570.
Khorramdel S, Khochaki A, Nasiri-Mahallati M and Ghorbani R. 2010. Effect of bofertilizer on yield and yield companent of nigella seed (Nigella sativa L.). Iranian Journal.of Field Crops Research, 8(5): 768-766. (In Persian).
Manafi H, Aliasgharzad N, Neyshabouri MR and Rejali F, 2010. Tolerance to Water Deficit Stress in Tomato Inoculated with Arbuscular Mycorrhizal Fungi. Journal Water and Soil Science, 22 (2): 97-117. (In Persian).
Mohammadi A, Bihamta MR and Dorri H. 2010. Effects of factors affecting cooking characteristics and protein content in 15 red bean genotypes (Phaseolus vulgaris L.) under normal irrigation and drought stress conditions. Iranian Journal of Pulses Research, (1) (2):143-152. (In Persian).
Mohammadi M, Malakouti MJ, Khavazi K, Rejali F and Davoodi MH. 2015. The Effect of Bio-fertilizer and Chemical Fertilizers (Phosphate and Zinc) onYield and Yield Components of Two Cultivars of Bean (Phaseolus vulgaris L.). Journal of Water and Soil, 29 (1) : 176-187. (In Persian).
Moradi A, Ahmadi A and HosseinZadeh A. 2008. Agro-Physiological Responses of Mung Bean (cv. Partov) to Severe and Moderate Drought Stress Applied at Vegetative and Reproductive Growth Stages. Journal of Water and Soil Science, 12 (45): 659-671. (In Persian).
Nikolaous N, Angelopoulos K and karagiannidis N. 2003. Effectes of drought stress on mycorrhizal and non-mycorrhizal cabernet sauvignon grapevine, graffed onto various rootstocks. Experimental Agriculture,39:241-252.
Oneill PM, Shanahan JF and Schepers JS. 2006. Use of chlorophyll fluorescence assessments to differentiate corn hybrid response to variable water conditions. Crop Science Society of Amrica, 46 (2): 681-687.
Sadat A, Savaghebi Gh, Rejali F, Farahbakhsh M, Khavazi K and Shirmardi M. 2010. Effects of some Arbuscular Mycorrhizal Fungi and Plant Growth Promoting Rhizobacteria on the growth and yield indices of two wheat varieties in a salinesoil. Journal of Water and Soil, 24 (1): 53-62. (In Persian).
Safapor M, Ardekani MR, Rejali F, KHagani S and Timori M. 2010. Effect of inoculation dual Mycorrhiza and Rhizobium on yield three varieties of red beans (Phaseolus vulgaris L.). Journal of New Findings in the Agriculture, 5: 21-35. (In Persian).
Safapor M K, Hagani S, Amir-Abadi M, Timori M and Kazem-Bzeyan M. 2009. The statistical study of the effects of water stress on phenology and agronomic traits white bean genotypes. Journal of New Findings in the Agriculture, 4: 367-378. (In Persian).
Sar-Abadani-Tafarreshi R, Bihamta MR, Sadat-Shier Z and Shahbazi M. 2013. Effect end season drought stress on yield and some physiological characteristics of some barley cultivars and lines. Journal of Water Research in Agriculture, 27(4): 535-549. (In Persian).
Schutz M and Fangmeir E. 2001. Growth and yield responses of spring wheat (Triticum aestivum L. cv. Minaret) to elevated CO2 and water limitation. Environmental Pollution, 114: 187-194.
Sobhani A, Shirani AH  and Nakhoda B, 1998. Guide book for determining crops Leaf Area Index. Extension booklet. SPII. Bio-technology Research department, P.13. (In Persian).
Suarez R, Wong A, Ramirez M, Barraza A, Orozco M, Cevallos M, Lara M, Hernandez G and Iturriaga G. 2008. Improvement of drought tolerance and grain yield in common bean by overexpressing trehalose-6-phosphate synthase in rhizobia. Molecular plant–microb interactions, 21:958-966
Vannozi G and Larner F. 2007. Proline accumulation during drought rhizogene in maise. Journal of Plant Physiology, 85: 441-467.
Yano-Melo AM, Saggin OJ and Maia LC. 2003. Tolerance of mycorrhized banana (Musa sp. cv. Pacovan) plantlets to saline stress. Agriculture Ecosystem Environment, 95:343-348.
Zarei M, Paymaneh Z, Ronaghi A, Kamgar-Haghighi AA and Shahsavar A. 2013. Effects of Arbuscular Mycorrhizal Fungus on Growth and Physiological Parameters of Rough Lemon Rootstock under Water Deficit Conditions. Journal of Water and Soil, 27 (3): 485-494. (In Persian).
Zang H, Pala M, Oweis Y and Harris H. 2000. Water use and water use effieiency of chickpea  and lentil in a mediterrraneam environment. Australian Journal of Agricultural Research, 51: 295-304.