The Effect of Pseudomonas fluorescens and Pseudomonas putida on Some Soil Biological Properties and Plant Growth Indices of Wheat under Salt Stress

Document Type : Research Paper

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Abstract

In order to study the effect of Pseudomonas fluorescens and P. putida on some soil biological properties and growth indices of wheat under salt stress and some of soil chemical and biological indices, a factorial experiment based on completely randomized design with three replications was conducted. The factors were salinity at four levels, control, 6, 8 and 10 dS/m, and inoculation with plant growth promoting rhizobacteria at three level, including no-inoculation, inoculation with Pseudomonas putida and inoculation with Pseudomonas fluorescens. The results showed that soil salinity significantly decreased plant yield parameters such as root volume, root fresh and dry weight, shoot fresh and dry weight, chlorophyll a, chlorophyll b, total chlorophyll and also the inoculation with Pseudomonas bacteria significantly increased these indices. Also, there was a significant difference between two Pseudomonas strains, however in general it was not possible to precisely compare two bacteria. On the other hand, the results showed that with increasing the salinity level, factors of soil biological properties such as microbial biomass carbon, basal respiration and substrate-induced respiration reduced and the use of bacteria has increased these traits. Regarding the soil biological indices that increased with the inoculation, it can be concluded that the inoculation indirectly increased soil biological indices by affecting plant growth and yield. Therefore, in the saline conditions plant growth promoting rhizobacteria could be used to directly increase plant growth and yield by plant promoting mechanisms and indirectly increase soil fertility condition and plant nutrition by increasing soil biological indices. 
 
 

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References

Aliasgharzad N. 2001. Labaoratory Methods in Soil Biology, Publication of University of Tabriz. (In Persian).
Anagholi A, Tabatabaii S and Fouman Ajirlou A. 2010. Evaluation of Salt-tolarance of varieties of Sorgom using sensitivity and stress-tolerance indices. Agricultural Crop Production 3(1): 89-102. (In Persian).
Kafi M, Lahooti M, Sharifi H and Goldani M. 1998. Plant Physiology. Publicarion of Mashhad Jahad Daneshgahi. (In Persian).
Rezaii M, Khavari Nejad R and Fahimi H. 2004. Investigaion of Physiologic Response of Cotton to Differnct Soil Salinities. Pajouhesh va Sazandegi in Agronomy and Horticutlture. 62:81-89. (In Persian).
Saadat A, Savaghebi Firooz Abadi GH, Rejali F, Farah Bakhsh M, Khavazi K and Shirmardi M. 2010. The effect of Arbuscular Mychorhizae and Plant Growth Promotind Rhizobacteria on Growth and Yield of Wheat in a Saline Soil. Soil and Water, 24(1):53-62. (In Persian).
Ali S, Charles TC and Glick BR, 2014. Amelioration of high salinity stress damage by plant growth-promoting bacterial endophytes that contain ACC deaminase. Plant Physiology and Biochemistry, 80:160-167.
Allakhverdiev S, Sakamoto A, Nishiyama Y, Inaba M and Murata N, 2000. Ionic and osmotic effects of NaCl-inactivation of photosystems I and II in Synechococcus spp. Journal of Plant Physiology, 123: 1047-1056.
Arnon AN, 1967. Method of extraction of chlorophyll in the plants. Agronomy Journal, 23:112-121.
Batra L and Manna MC, 1997. Dehydrogenase activity and microbial biomass carbon in salt‐affected soils of semiarid and arid regions. Arid Land Research and Management, 11(3): 295-303.
Chander K, Goyal S and Kapoor KK, 1994. Effect of sodic water irrigation and farm yard manure application on soil microbial biomass and microbial activity. Applied Soil Ecology, 1: 139-144.
Colmer TD, Flowers TJ and Munns R, 2006. Use of wild relative to improve salt tolerance in wheat. Journal of Experimental Botany, 57: 1059-1078.
Cronin D, Moënne-Loccoz Y, Fenton A, Dunne C, Dowling DN and O'Gara F, 1997. Ecological intraction of a biocontrol Pseudomonas fluorescens strain producing 2,4-diacetylphloroglucinolwith the soft rot potato pathogen Erwinia carotovora subsp. Atroseptica. FEMS Microbiolgy Ecology, 23: 95-106.
Desingh R and Kanagaraj G, 2007. Influence of salinity stress on photosynthesis and antioxidative systems in two cotton varieties. General and Applied Plant Physiology, 33: 221-234.
Drazkiewicz M, 2000. Chlorophyllase: occurrence, functions, mechanism of action, effects of external and internal factors. Photosynthesis, 30: 321-331.
Glick BR, Donna Penrose M and Jiping L, 1998. A model for the lowering of plant ethylene concentrations by plant growth-promoting bacteria. Journal of Theoretical Biology, 190: 63-68.
Gupta PK, 2004. Soil, Plant, Water and fertilizeranalysis. Agrobios. India
Han HS and Lee KD 2005. Plant growth promoting rhizobacteria effect on antioxidant status, photosynthesis. mineral uptake and growth of lettuce under soil salinity. Agriculture and Biological Sciences, 1: 210-215.
Jones JR and Benton J, 2001. Laboratory guide for conducting soil tests and plant analysis. CRS Press LLC. U.S
Lal Khajanchi SG, Setih M, Sharma PC, Swarup A and Gupta SK, 2007. Effect of NaCl concentration ongrowth, root morphology and photosynthetic pigment in wheat and barley under solution culture. Journal of Agrochimical, 51: 194-206.
Mayak S, Tirosh T and Glick BG, 2004. Plant Growth promoting bacteria confer resistance in tomato plants salt stress. Plant Physiology and Biochemestry, 42:565-572.
Nadeem S, Zahir ZA, Naveed M and Arshad M, 2007. Preliminary inverstigations on inducing salt tolerance in Canola through ACC-deaminase activity. Canadian Journal of Microbiology, 53(10):1141-1149.
Nelson DW and Sommers L, 1982. Total carbon, organic carbon, and organic matter. Methods of soil analysis. Part 2. Chemical and microbiological properties, Soil society of America. Inc, Madison, WI.
Olsen SR and Sommers LE, 1982. Phosphorus, Methods of soil analysis, Agronomy series No 9, Part 2. Soil society of America. Inc, Madison, WI.
Pathak H and Rao DLN, 1998. Carbon and nitrogen mineralization from added organic matter in saline and alkali soils. Soil Biology Biochem, 30: 695–702.
Reddy MP and Vora AB, 2005. Salinity induced changes in pigment composition and chlorophyllase activity of chelidonium. IndianJournalPlantPhysiology, 29: 331-334.
Rietz DN and Haynes RJ, 2003. Effect of irrigation-induced salinity and sodicity on soil microbial activity. Soil Biology Biochemictry, 35:845-854.
Saravanakumar D and Samiyappan R, 2007. ACC deaminase from Pseudomonas fluorescens mediated saline resistance in groundnut (Arachis hypogea) plants. Journal of Applied Microbiology, 120: 1283-1292.
Saviozzi A, Cardelli R and Di Puccio R, 2011. Impact of salinity on soil biological activities: a laboratory experiment. Communications in Soil Science and Plant Analysis, 42(3): 358-367.
Sharma AK, 2003. Biofertilizers for sustainable agriculture. Agrobios, India.
Shrivastava P and Kumar R, 2014. Soil salinity: A serious environmental issue and plant growth promoting bacteria as one of the tools for its alleviation. Saudi Journal of Biological Sciences, 22(2): 123-131.
Tripathi S, Kumari S, Chakraborty A, Gupta A, Chakrabarti K and Bandyapadhyay BK, 2006. Microbial biomass and its activities in salt-affected coastal soils. Biology fertilizers Soils, 42: 273-277.
Zahir ZA, Arshad M and Frankernberger WT, 2004. PGPR: Application and perspectives in agriculture. Advances in Agronomy, 81:97-167.
Zeng WZ, Xu C, Wu JW, Huang JS and Ma T, 2013. Effect of salinity on soil respiration and nitrogen dynamics. Ecological Chemistry and Engineering S, 20(3): 519-530.
JOURNAL OF AGRICULTURAL SCIENCE AND SUSTAINABLE PRODUCTION
Volume 27, Issue 4
January 2018
Pages 65-79

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