Collection and Identification of Native Rhizobium Strains and Assessment of their Symbiosis with Progressive Persian Clover Lines

Document Type : Research Paper

Authors

Abstract

In order to introducing efficient ecological genotype and native rhizobium strains in persian clover (Trifolium resupinatum L.) this study was conducted during 2003-2011. Since 2003, native persian clover populations were collected in Lorestan province, Iran, and after different studies15 promising lines were selected.Then, 15 native Rhizobium legominosarum bv. trifollii strains were collected and identified from the same area. In 2010, a greenhouse factorial experiment based on randomized complete design in three replications was conducted with 240 treatments (15 line × 16 strain) in research station of Borujerd. In this study symbiosis index, root and above-ground dry matters, root:shoot proportion, stem height, nodule number and nitrogen content of plant tissue were assessed. Grouping lines and strains was performed by cluster analysis under Ward method. Results showed that interaction of line × strain were significant (p<0.01) on all of studied traits. Canonical discriminate analysis was useful in describing the variation among genotypes and strains. Cluster analysis was successful in differentiating the varieties into similar sub-groups based on the measured traits. Regarding to cluster analysis the RTB2 and line No.13 were determined as a better selection with view to studied traits.
 
 

Keywords


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