Collection and Identification of Native Rhizobium Strains and Assessment of their Symbiosis with Progressive Persian Clover Lines

Shahverdi, Mohamad; Mirshekari, Bahram; Asadi Rahmani, Hadi; Ardakani, Mohammad Reza; Rashidi, Varharam

Collection and Identification of Native Rhizobium Strains and Assessment of their Symbiosis with Progressive Persian Clover Lines

Document Type : Research Paper

Authors

Abstract

In order to introducing efficient ecological genotype and native rhizobium strains in persian clover (Trifolium resupinatum L.) this study was conducted during 2003-2011. Since 2003, native persian clover populations were collected in Lorestan province, Iran, and after different studies15 promising lines were selected.Then, 15 native Rhizobium legominosarum bv. trifollii strains were collected and identified from the same area. In 2010, a greenhouse factorial experiment based on randomized complete design in three replications was conducted with 240 treatments (15 line × 16 strain) in research station of Borujerd. In this study symbiosis index, root and above-ground dry matters, root:shoot proportion, stem height, nodule number and nitrogen content of plant tissue were assessed. Grouping lines and strains was performed by cluster analysis under Ward method. Results showed that interaction of line × strain were significant (p<0.01) on all of studied traits. Canonical discriminate analysis was useful in describing the variation among genotypes and strains. Cluster analysis was successful in differentiating the varieties into similar sub-groups based on the measured traits. Regarding to cluster analysis the RTB₂ and line No.13 were determined as a better selection with view to studied traits.

Keywords

References

Akbari G, Allahdadi A, Dashti Sh and Aghaalikhani H, 2012. Soil Microbiology (Microorganisms and plant growth). Publication of Tehran University. (In Persian).

Antoun H, Beauchamp CJ, Goussard N, Chabot R and Lalande R, 2002. Potential of Rhizobium and Brady Rhizobium species as plant growth promoting Rhizo-bacteria on non-legumes: effect on radish (Raphanus sativus L.). Plant and Soil, 204: 57-67.

Asadi Rahmani H., Asgharzadeh A, Khavarzi K and Savagebi G, 2007. Biological productivity of soil. Publication of Jehad e Daneshgahi, (In Persian).

Bagnasco P, 1998. Fluorescent pseudomonas: as a bio-control agents against forage legume root pathogenic fungi, Soil Biology and Biochemistry, 30: 1317-1322.

Beck DP, Materon LA and Afandi F, 1993. Practical Rhizobium-Legume technology manual. Technical manual. 19: ICARDA, Alepo, Syria.

Biswas JC, Ladha JK, Dazzo FB, Yanni YG and Rolfe BG, 2000. Rhizobial inoculation influences seedling vigor and yield of rice. Agronomy Journal, 92: 880-886.

Chabot R, Antoun H and Cescas M, 1996. Growth promotion of maize and lettuce by phosphate-solubilizing Rhizobium leguminosarum biovar phaseoli. Plant and Soil, 184: 311-321.

Daei D, Ardakani MR, Regali F and Teimori S, 2009. Alleviation of salinity stress on wheat yield and nutrient uptake using AM fugi under field condition. Journal of Plant Physiology, 166: 617-625.

Dakora FD, 2003. Defining new roles for plant and Rhizobial molecules in sole and mixed plant cultures involving symbiotic Legumes. New Phytology, 157: 39-49.

Dakora FD, Matiru VN, King M and Phillips DA, 2002. Plant growth promotion in legumes and cereals by lumichrome, a rhizobial signal metabolite. Publication of Wallingford UK: CABI.

Erice W and Terses BM, 1987.Trifolitoxin production and nodulation are necessary for the expression of superior nodlutaion competitiveness by Rhizobium legominosarumbv.trifollii strain T₂₄ on clover. Plant Physiology, 85: 335-342.

Golipour H, Nabizadeh A and Nasrollahzadeh A, 2007. Study of nitrogen fixation among native Rhizobium lines in annual lucernes. Iranian Journal of Field Crop Research, 2(2): 78-83. (In Persian).

Gonzalez-Lopez J, Martinez-Toledo MV, Rodelas B, Pozo C and Salmeron V, 1995. Production of aminoacids by free-living heterotrophic nitrogen-fixing bacteria. Journal of Plant Physiology, 124: 15-21.

Goto I and Minson DJ, 1977. Prediction of the dry matter digestibility of tropical grasses using a Pepsin cellulase assay. Animal Feed Science and Technology, 2: 247-253.

Hega K and Lino M, 1998. Auxin-growth relationships in maize coleoptiles and pea internodes and control by auxin of the tissue sensitivity to auxin. Plant Physiology, 117: 1473-1486.

Hoflich G, Wiehe W and Kihn G, 1994, Plant growth stimulation by inoculation with symbiotic and associative rhizosphere micro-organisms. Experimentia, 50: 897-905.

Kaneshiro T, and Kwoleck W, 1985. Stimulated nodulation of soybean by Rhizobium japonicum mutant (B-14075) that catabolizes the convection of tryptophan to indol-acetic acid. Plant Science, 42: 141-146.

Khosravi H, Khavarzi K and Mirzashahi K, 2001. Application of inoculum instead of urea fertilizer in Vicia faba in Safiabad of Dezful. Journal of Soil and Water, 12: 244-261. (In Persian).

Kremer R, and Souissi T, 2001.Cyanide production by Rhizo-bacteria and potential for suppression of weed seedling growth. Microbiology, 43: 182-186.

Ligero F, Caba JM, Lluch C and Olivares J, 1986. Evolution of ethylene from roots of Medicago sativa plants inoculated with Rhizobium meliloti. Plant Physiology, 125: 361-365.

Meghvansi MK, Kamal P and Mahna SK, 2005. Identification of PH tolerant Brady Rhizobium Japonicum strains and their symbiotie effectiveness in soybean [Glycine max (L.) Merr] in low nutrient soil. African Journal of Biotechnology, 4: 633-666.

Noel T, Shen C, Yost CK, Pharis RP and Hynes MF, 1996. Rhizobium leguminosarum as a plant growth promoting Rhizo-bacterium: Direct growth promotion of canola and lettuce. Microbiology, 42: 279-283.

Okereke GR, Onochie CC, Onvkwo AU, Onyeagba E and Ekejindu GD, 2000. Response of introduced Brady Rhizobium strains infecting a promiscuous soybean cultivar. World Journal of Microbiology and Biotechnology,16: 43-48.

Shahverdi M and Zamanian M, 2007. Evaluation of forage yield in late ripening of Iranian clover. Publication of Institute of Seed and Plant improvement of Karaj. (In Persian).

Shahverdi M and Zamanian M, 2009. Evaluation of forage yield and other characteristics in late ripening of Iranian clover. Publication of Institute of Seed and Plant improvement of Karaj. (In Persian).

Shahverdi M, Mirshekari B, Asadi Rahman, H., Rashidi V and Ardakani MR, 2012. Effect of biological and reduced rates of chemical fertilizers on forage yield (quantity and quality) in Persian clover. Journal of Beh-Zeraei Keshavarzi, 16(2): 271-287.(In Persian).

Shahverdi M, Zamanian M and Soleymanpour SH, 2009. Evaluation of forage yield and its sustainability in late ripening of Iranian clover. Publication of Institute of Seed and Plant improvement of Karaj. (In Persian).

Wilson JR, 1994. Cell wall characteristics in relation to forage digestion by ruminants. Journal of Agricultural Science, 122: 173-182.

Yadgari M, Akbari G and Asadi Rahmani H, 2004. Effect of soybean seed inoculation with different strains of bacteria on nodulation and nitrogen fixation. Iranian Journal of Agronomy Sciences, 6 (1): 35-52. (In Persian).

Zahir AZ, Abbas SA, Khalid A and Arshad M, 2000. Substrate dependence microbial derived plant hormones for improving growth of maize seedlings. Pakistan Journal of Biological Sciences, 3: 289-291.

Zamanian M, 2002. Persian Clover. Publication of Seed and Plant Improvement Institute of Karaj. (In Persian).

JOURNAL OF AGRICULTURAL SCIENCE AND SUSTAINABLE PRODUCTION

Article View: 416
PDF Download: 721

Collection and Identification of Native Rhizobium Strains and Assessment of their Symbiosis with Progressive Persian Clover Lines

References

Akbari G, Allahdadi A, Dashti Sh and Aghaalikhani H, 2012. Soil Microbiology (Microorganisms and plant growth). Publication of Tehran University. (In Persian).

Asadi Rahmani H., Asgharzadeh A, Khavarzi K and Savagebi G, 2007. Biological productivity of soil. Publication of Jehad e Daneshgahi, (In Persian).

Golipour H, Nabizadeh A and Nasrollahzadeh A, 2007. Study of nitrogen fixation among native Rhizobium lines in annual lucernes. Iranian Journal of Field Crop Research, 2(2): 78-83. (In Persian).

Khosravi H, Khavarzi K and Mirzashahi K, 2001. Application of inoculum instead of urea fertilizer in Vicia faba in Safiabad of Dezful. Journal of Soil and Water, 12: 244-261. (In Persian).

Shahverdi M and Zamanian M, 2007. Evaluation of forage yield in late ripening of Iranian clover. Publication of Institute of Seed and Plant improvement of Karaj. (In Persian).

Shahverdi M and Zamanian M, 2009. Evaluation of forage yield and other characteristics in late ripening of Iranian clover. Publication of Institute of Seed and Plant improvement of Karaj. (In Persian).

Shahverdi M, Mirshekari B, Asadi Rahman, H., Rashidi V and Ardakani MR, 2012. Effect of biological and reduced rates of chemical fertilizers on forage yield (quantity and quality) in Persian clover. Journal of Beh-Zeraei Keshavarzi, 16(2): 271-287.(In Persian).

Shahverdi M, Zamanian M and Soleymanpour SH, 2009. Evaluation of forage yield and its sustainability in late ripening of Iranian clover. Publication of Institute of Seed and Plant improvement of Karaj. (In Persian).

Yadgari M, Akbari G and Asadi Rahmani H, 2004. Effect of soybean seed inoculation with different strains of bacteria on nodulation and nitrogen fixation. Iranian Journal of Agronomy Sciences, 6 (1): 35-52. (In Persian).

Volume 28, Issue 1
April 2018
Pages 65-77

Files

Share

How to cite

Statistics

Collection and Identification of Native Rhizobium Strains and Assessment of their Symbiosis with Progressive Persian Clover Lines

References

Akbari G, Allahdadi A, Dashti Sh and Aghaalikhani H, 2012. Soil Microbiology (Microorganisms and plant growth). Publication of Tehran University. (In Persian).

Asadi Rahmani H., Asgharzadeh A, Khavarzi K and Savagebi G, 2007. Biological productivity of soil. Publication of Jehad e Daneshgahi, (In Persian).

Golipour H, Nabizadeh A and Nasrollahzadeh A, 2007. Study of nitrogen fixation among native Rhizobium lines in annual lucernes. Iranian Journal of Field Crop Research, 2(2): 78-83. (In Persian).

Khosravi H, Khavarzi K and Mirzashahi K, 2001. Application of inoculum instead of urea fertilizer in Vicia faba in Safiabad of Dezful. Journal of Soil and Water, 12: 244-261. (In Persian).

Shahverdi M and Zamanian M, 2007. Evaluation of forage yield in late ripening of Iranian clover. Publication of Institute of Seed and Plant improvement of Karaj. (In Persian).

Shahverdi M and Zamanian M, 2009. Evaluation of forage yield and other characteristics in late ripening of Iranian clover. Publication of Institute of Seed and Plant improvement of Karaj. (In Persian).

Shahverdi M, Mirshekari B, Asadi Rahman, H., Rashidi V and Ardakani MR, 2012. Effect of biological and reduced rates of chemical fertilizers on forage yield (quantity and quality) in Persian clover. Journal of Beh-Zeraei Keshavarzi, 16(2): 271-287.(In Persian).

Shahverdi M, Zamanian M and Soleymanpour SH, 2009. Evaluation of forage yield and its sustainability in late ripening of Iranian clover. Publication of Institute of Seed and Plant improvement of Karaj. (In Persian).

Yadgari M, Akbari G and Asadi Rahmani H, 2004. Effect of soybean seed inoculation with different strains of bacteria on nodulation and nitrogen fixation. Iranian Journal of Agronomy Sciences, 6 (1): 35-52. (In Persian).

Volume 28, Issue 1April 2018Pages 65-77

Files

Share

How to cite

Statistics

Volume 28, Issue 1
April 2018
Pages 65-77