The Effect of Trichoderma Isolates on Tomato Growth and Nutrients Uptake under Water Deficit Stress

Document Type : Research Paper

Authors

Abstract

Water shortage is one of the most important limiting factors for plant growth around the world. To cope with this phenomenon, several solutions have been proposed, including the use of microorganism with plant growth promoting traits such as Trichoderma. Accordingly, an experiment was conducted to investigate the effect of three fungal species on tomato growth factors, as well as nutrients uptake by the plant in water deficit stress conditions in a factorial arrangement based on randomized complete block design. Treatments including T1 (Trichoderma longibrachiatum KH), T2 (T. longibrachiatum MA), T3 (T. harzianum), NT1 (negative control - without fungi) and NT2 (positive control – without fungi and with chemical fertilizer) at three moisture levels consist of W0 (none stress), W1 (medium stress) and W2 (severe stress), performed in three replications. The results indicated that dry weight of shoot and root and nutrients’ uptake decreased in all treatments as water stress increased, but this reduction was significantly lower in fungal treatments. Under none stress condition (W0), T1 and T2 treatments increased N and P absorption and T3 fungal treatments increased Fe and Zn absorption significantly. By increasing stress conditions, most of the measured parameters of the plant in fungal treatments were reduced except for T1 treatment. Under severe stress condition (W2), T1 enhanced dry weight (32.8%), N uptake (62.7%), P (34.78%), Fe (39.3%) and Zn (47.6%), compared to negative control.
 

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Altomare C, Norvell WA, Björkman T and Harman GE. 1999. Solubilization of phosphates and micronutrients by the plant-growth-promoting and biocontrol fungus Trichoderma harzianum Rifai 1295-22. Applied and Environmental Microbiology, 65:2926-2933.
Ashraf A and Mehmood S. 1990. Response of four Brassica species to drought stress. Environmental and Experimental Botany, 30: 93-100.
Augé RM. 2001. Water relations, drought and vesicular-arbuscular mycorrhizal symbiosis. Mycorrhiza,
11:3–42.
 Azarmi R, Hajieghrari B and Giglou A. 2011. Effect of Trichoderma isolates on tomato seedling growth response and nutrient uptake.  African Journal of Biotechnology, 10: 5850–5855.
Campbell GS and Gee GW. 1986. Water potential: miscellaneous methods. Pp. 619-633. In: Methods of Soil Analysis: Part 1—Physical and Mineralogical Methods, (methodsofsoilan1).
Chacon MR, Rodriguez-Galan O, Benitez T, Sousa S, Rey M, Llobell A and Delgado-Jarana J. 2007. Microscopic and transcriptome analyses of early colonization of tomato roots by Trichoderma harzianum. International Microbiology, 10: 19-27.
Cottenie A. 1980. Soil and Plant Testing as a Basis of Fertilizer Recommendation. FAO soils bulletin. 38: 94-100.
Daszkowska-Golec A and Szarejko I. 2013. Open or close the gate–stomata action under the control of phytohormones in drought stress conditions. Frontiers in Plant Science. 4: 138-145
Ekhvat M and Karimpour F. 1996. The effect of several antagonist fungi against Fusarium solani black Root caries of chickpea in greenhouse conditions. Journal of Agricultural Science, 27: 37 - 45. (In Persian).
Elander K and Mukherji R. 1992. Fungal biotechnology, in: Handbook of Applied Mycology, Markel Dekker, New York. p. 4.
Elad Y, Lifshitz R and Baker R. 1985. Enzymatic activity of the mycoparasite Pythium nunn during interaction with host and non-host fungi. Physiological Plant Pathology, 27: 131-148.
Gee GW and Or D. 2002. Particle Size Analysis. In: Dane JH and Topp GC, Eds., Methods of Soil Analysis, Part 4, Physical Methods, Soils Science Society of America, Book Series No. 5, Madison, 255-293.
Gupta PK. 2000. Soil Plant Water and Fertilizer Analysis. Agrobios pub. Bikaner. India.
Hafeez B, Khanif YM and Saleem M. 2013. Role of zinc in plant nutrition-a review. American journal of Experimental Agriculture, 3: p.374.
Hibbett DS, Binder M, Bischoff JF, Blackwell M, Cannon PF, Eriksson OE, Huhndorf S, James T, Kirk PM, Lücking R and Lumbsch HT. 2007. A higher-level phylogenetic classification of the Fungi. Mycological Research, 111:509-47.
Khoshmanzar E. 2015. Effects of Trichoderma isolates on tomato growth and tolerance to water deficit stress in a loamy sand soil, Master's thesis, Faculty of Agriculture, Tabriz University. (In Persian).
Malekoti M. 2000. Optimized fertilizer recommendation for Crops and Gardening. Technical Journal No. 200. Water and Soil Research Institute, Agricultural Education Publishing. (In Persian).
Mohammadi Kh, Ghalavand A, Aghahalkhani M, Sohrabi Y and Heidari GR. 2010. Influens of chickpea seeds quality of increasing soil fertility different systems. Journal of Crop Production, 3: 103-119. (In Persian).
Nzanza B, Marais D and Soundy P. 2012. Yield and nutrient content of tomato (Solanum lycopersicum L.) as influenced by Trichoderma harzianum and Glomus mosseae inoculation. Science Horticulture. 144: 55-59
Olsen SR and Sommers LE. 1982. Phosphorus. pp 403-430. In: Page AL, Methods of Soil Analysis, Part 2. American Society of Agronomy٫ Soil Science Society of America. Madison, Wisconsin.
Qi W and Zhao L. 2013. Study of the siderophore-producing Trichoderma asperellum Q1 on cucumber growth promotion under salt stress.  Basic Microbiology. 53: 355–364.
Page AL. 1982. Methods of Soil Analysis. Part 2. Chemical and Microbiological Properties. Soil Science Society of America.
Li RX, Cai F, Pang G, Shen QR, Li R and Chen W. 2015. Solubilisation of phosphate and micronutrients by Trichoderma harzianum and its relationship with the promotion of tomato plant growth. PLoS One. 10(6): e013008
Rowell D. 1994. Soil Science: Method and Application. Longman Scientific and Technical, Wiley, UK. P. 350.
Rudresh DL, Shivaprakash MK and Prasad RD. 2014. Effect of combined application of Rhizobium, phosphate solubilizing bacterium and Trichoderma spp. on growth, nutrient uptake and yield of chickpea (Cicer aritenium L.) Applied Soil Ecology. 28 :139-146.
Sainz MJ, Taboada-Castro MT and Vilarino A. 1998. Growth, mineral nutrition and mycorrhizal colonization of  red clover and cucumber plants grown in a soil amended with composted urban wastes. Plant and Soil. 205: 85-92.
Shoresh M, Mastouri F and Harman GE. 2010. Induced systemic resistance and plant responses to fungal biocontrol agents. Annual Review of Phytopathology.  48: 21–43.
Singh J and Patal A. 1996. Water Statues, gaseous exchange, proline accumulation and yield of wheat in response to water stress. Annual of Biology Ludhiana 12: 77-81.
Singh V, Singh P, Yadav R, Awasthi S, Joshi B, Singh R, Lal R and Duttamajumder S. 2010. Increasing the efficacy of Trichoderma harzianum for nutrient uptake and control of red rot in sugarcane. Journal of Horticulture and Forestry 2: 66–71.
Shukla N, Awasthi RP, Rawat L and Kumar J. 2012. Biochemical and physiological responses of rice (Oryza sativa L.) as influenced by Trichoderma harzianum under drought stress. Plant Physiology and Biochemistry, 54:78-88.
Taekim J, Park IH, HahumYI and Hun Yu S. 2001. Crown and root rot of greenhouse tomato caused by Fusarium oxysporum. Plant Pathology, 17: 299-294.
Waling I, Vark W, Houba V and Van J. 1989. Soil and Plant Analysis, a series of syllabi. Part 7. Plant Analysis Procedures. Wageningen Agriculture University, Netherland.
Westerman G. 1990. Soil Testing and Plant Analysis. Soil Science Society of America. INC. Madison, Wisconsin, USA.
Westgate ME. 1994. Water statues and development of the maize endosperm and embryo during drought. Crop Science, 34: 76-83.
Woo SL, Scala F, Ruocco M and Lorito M. 2006. The molecular biology of the interactions between Trichoderma spp. phytopathogenic fungi, and plants. Phytopathology. 96: 181-185.
Li YT, Hwang SG, Huang YM and Huang CH. 2018. Effects of Trichoderma asperellum on nutrient uptake and Fusarium wilt of tomato. Crop Protection, 110:275-82.