Induction of Salt Tolerance in Canola by Inoculation with Pseudomonas flourescens FY32

Document Type : Research Paper

Authors

Abstract

Ability of Pseudomonas flourescens FY32 in growth improving and resistance increasing against salinity stress in strain SW5001, was studied by split plot experiment based on completely randomized design with three replications under hydroponic conditions. The first factor was salinity stress as three levels (0, 150 and 300 mM NaCl), the second factor was bacteria inoculation as two levels (inoculation and non- inoculation), and the third factor was two canola cultivars (SW5001 and Comet). The results showed that, under salt stress conditions, inoculated plants have better growth characteristics than inoculated plants. There was a significant difference between the two varieties SW5001 and Comet Sodium leaf increased with raising salinity, but this increment in the medium stress level (150 mM) and in the severe stress level (300 mM) in inoculated plants decreased significantly compared to inoculated plants, which indicates the positive effect of canola inoculated with bacteria in reducing the negative effects of salinity stress. The interaction of salinity and two canola cultivars was significant for these ions. However, Comet showed two levels of stress with the highest sodium absorption, indicating the susceptibility of this variety to salinity stress versus SW5001.Although increasing salinity was effective in increasing the sodium concentration of canola leaves. The interaction of salinity and cultivar was also significant in the potassium trait. SW5001 had the highest concentration of potassium which indicates the higher ability of this amount to absorb this ion and maintain the growth of this figure relative to the Comet variety at different levels of salinity. Potassium in leaves under salinity stress. SW5001 was arranged tolerance to salinity that Comet. Inoculation with bacteria reduced the sodium content and increased potassium in both cultivars.
 

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