Study the Survival of Enterobacter cloacae Bacteria in Several Solid Carriers and Effect of Prepared Inoculants on Ggermination and Growth of Wheat

Document Type : Research Paper

Authors

Abstract

         Biofertilizers play major role in sustainable agriculture. Therefore, to provide them, different carriers are used to increase the longevity and survival of the bacteria. The aim of this study was to investigate the survival of Enterobacter cloacae bacteria on different solid carriers during one year. Solid carriers consist of 10 treatments of bagasse, peat, hydrochar, biochar, sawdust and perlite as a single treatment and mixed with perlite (in a ratio of 1:1). In this study, bacterial inoculants prepared with the initial population (109 CFU/g) after storage at room temperature were compared for the survival of the bacteria. The bacterial population was counted at 0, 15, 30, 60, 90, 120, 180, 270 and 365 days. For counting the bacteria in microbial carriers, after dilution series preparation, bacterial suspension was used in strip culture in a plate. In this research, the effects of prepared inoculants on germination and growth of wheat seedlings in sterile conditions in a plate culture at the end of the fourth month were investigated. In these method, characteristics such as shoot and root length, the wet and dry weight of shoot and root, total wet and dry weight of shoot and root were measured.The results of bacterial count showed that among the tested carriers, the most population counted after one year in bagasse carrier (109 CFU/g) and the lowest population was counted in the sawdust, so that after 6 months no alive cells of bacteria were counted in sawdust. Also, the results of germination test and growth wheat seedling growth showed that in most of the measured characteristics the hydrochar and bagasse + perlite in both plate experiment, had reproducible results and they had better means. Concluding, according to the results of this experiment and the convenience and availability of the carriers, bagasse + perlite carrier had the best results in increasing the survival of the bacteria and we suggest this kind of carrier.


 

 
 
 

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Abit SM, Bolster CH, Cai P and Walker SL. 2012. Influence of feedstock and pyrolysis temperature of biochar amendments on transport of Escherichia coli in saturated and unsaturated soil. Environmental Science and Technology,46(15): 8097-8105.
Amonette JE and Joseph S. 2012. Characteristics of biochar: microchemical properties. In biochar for environmental management: Science and Technology, 65-84.
Azimzadeh Y and Najafi N. 2016. Effects of biochar on soil physical, chemical and biological properties. Land Management Journal, 4(2): 161-173. (In Persian).
Bashan Y. 1998. Inoculants of plant growth-promoting bacteria for use in agriculture. Biotechnology Advances, 16(4): 729-770.
Bais HP, Vepachedu R, Gilroy S, Callaway RM and Vivanco JM. 2003. Allelopathy and exotic plant invasion: from molecules and genes to species interactions. Science, 301(5638): 1377-1380.
Besharati H, Saleh Rastin N, Malakouti MJ and Alizadeh A. 2004. Study of Thiobacilli survival on different carriers. Journal of Soil and Water Sciences, 18(2): 168-178. (In Persian).
Chen B, Zhou D and Zhu L. 2008. Transitional adsorption and partition of nonpolar and polar aromatic contaminants by biochars of pine needles with different pyrolytic temperatures. Environmental Science and Technology, 42(14): 5137-5143.
Chun Y, Sheng G, Chiou CT and Xing B. 2004. Compositions and sorptive properties of crop residue-derived chars. Environmental Science and Technology, 38(17): 4649-4655.
Chao WL and Alexander M. 1984. Mineral soils as carriers for Rhizobium inoculants. Applied and Environmental Microbiology, 47(1): 94-97.  
Castaldi S, Riondino M, Baronti S, Esposito F, Marzaioli R, Rutigliano F and Miglietta F. 2011. Impact of biochar application to a Mediterranean wheat crop on soil microbial activity and greenhouse gas fluxes. Chemosphere, 85(9): 1464-1471.
Egamberdiyeva D, Juraeva D, Poberejskaya S, Myachina O, Teryuhova P, Seydalieva L and Aliev A. 2004. Improvement of wheat and cotton growth and nutrient uptake by phosphate solubilizing bacteria. In Proceeding of 26th Annual Conservation Tillage Conference for Sustainable Agriculture. Auburn, Pp. 58-65.
Egamberdieva D, Reckling M and Wirth S. 2017. Biochar-based Bradyrhizobium inoculum improves growth of lupin (Lupinus angustifolius L.) under drought stress. European Journal of Soil Biology, 78: 38-42.
Fages J. 1992. An industrial view of Azospirillum inoculants: formulation and application technology. Symbiosis-Rehovot, 12: 15-15.
Gandhi A and Sivakumar K. 2010. Impact of vermicompost carrier based bioinoculants on the growth, yield and quality of rice (Oryza sativa L.) CV NLR 145. The Ecoscan, 4(1): 83-88.
Huber D, El-Nasshar H, Moore L, Mathre D and Wagner J. 1989. Interaction between a peat carrier and bacterial seed treatments evaluated for biological control of the take-all diseases of wheat (Triticum aestivum L.). Biology and Fertility of Soils, 8(2): 166-171.
Kazemi Oskuei BK, Bandehagh A, Sarikhani MR. and Komatsu S. 2017. Protein profiles underlying the effect of plant growth-promoting rhizobacteria on canola under osmotic stress. Journal of Plant Growth Regulation, 37(2): 560-574.
Khavarzi K and Rejali F. 2000. Use of inexpensive and local materials as carriers of Bradyrhizobium Japonicum bacteria. Journal of Soil and Waters Sciences, 14(1): 36-45. (In Persian).
Leach A and Mumford J. 2008. Pesticide environmental accounting: A method for assessing the external costs of individual pesticide applications. Environmental Pollution, 151(1): 139-147.
Muhammad N, Dai Z, Xiao K, Meng J, Brookes PC, Liu X and Xu J. 2014. Changes in microbial community structure due to biochars generated from different feedstocks and their relationships with soil chemical properties. Geoderma, 226: 270-278.
Moradi Sh and Sarikhani MR. 2016. Comparison of dissolution of phosphate from sources of phosphate rock and Tricalcium phosphate by some phosphate solubilizing bacteria. Second National Congress for the Development of  agricultural science and Natural Resources, Gorgan. Iran, 1-6. (In Persian).
Mashhadi Asghari S and Aliasgharzadeh N. 2005. Comparison of five carriers of Sinorrhizobium meliloti to produce alfalfa inoculant. Journal Water and Soil Sciences, 8(4): 63-75. (In Persian).
Malakooti MJ. 1994. Sustainable agriculture and increasing yield by optimizing fertilizer use in Iran. Tehran Agricultural Education Publication, 460. (In Persian).
Owlia P, Fatemi AZ, Salimi H, Rasooli I, Sadrnia M, and Armandzadegan M. 2013. Evaluation of appropriate carriers for bio-control agents of apple fire blight. Egyptian Journal of Biological Pest Control, 23(1): 31.
Page AL, Miller RH and Keeney DR. 1982. Method of soil analysis. part 2- chemical and microbiological properties. 2nd ed., ASA pub. Buxton, Madison, WI, 159-166.
Rivera‐Utrilla J, Bautista‐Toledo I, Ferro‐García MA. and Moreno‐Castilla C. 2001. Activated carbon surface modifications by adsorption of bacteria and their effect on aqueous lead adsorption. Journal of Chemical Technology and Biotechnology, 76(12): 1209-1215.
Roughley R. 1976. The production of high quality inoculants and their contribution to legume yield.    Symbiotic Nitrogen Fixation in Plants, 7(1): 125.
Stephens J and Rask H. 2000. Inoculant production and formulation. Field Crops Research, 65(2): 249-258.
Smith R. 1992. Legume inoculant formulation and application. Canadian Journal of Microbiology, 38(6): 485-492.  
Sarikhani MR. 2016. Practical methods for the quality control of inoculant biofertilizers. Morteza Dasht Publication, 58-63. (In Persian).  
Salem M, Kohler J, Wurst S and Rillig MC. 2013. Earthworms can modify effects of hydrochar on growth of Plantago lanceolata and performance of arbuscular mycorrhizal fungi. Pedobiologia, 56(4): 219-224.
Samonin V and Elikova E. 2004. A study of the adsorption of bacterial cells on porous materials. Microbiology, 73(6): 696-701.
Sekar KR and Karmegam N. 2010. Earthworm casts as an alternate carrier material for biofertilizers: assessment of endurance and viability of Azotobacter chroococcum, Bacillus megaterium and Rhizobium leguminosarum. Scientia Horticulturae, 124(2): 286-289.
Shariati Sh and Alikhani A. 2014. The application of Pseudomonas fluorescens bacteria inoculants on certain growth indices and nutrient uptake in maize. Journal of Agricultural Knowledge and Sustainable Production, 24(4): 46-59. (In Persian).
Van Elsas JD and Heijnen C. 1990. Methods for the introduction of bacteria into soil: a review. Biology and Fertility of Soils, 10(2): 127-133.