اثرات محلول پاشی سالیسیلیک اسید و کینتین بر واکنش های فیزیولوژیکی سویا در سطوح مختلف تنش شوری

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه مهندسی تولید و ژنتیک گیاهی دانشکده کشاورزی دانشگاه ارومیه

2 تولید و ژنتیک گیاهی/ هیات علمی

3 گروه مهندسی کامپیوتر، دانشکده برق و کامپیوتر، دانشگاه ارومیه

10.22034/saps.2024.60335.3178

چکیده

اهداف: این تحقیق به منظور بررسی تأثیر کینتین و سالیسیلیک اسید بر کاهش تنش‌های یونی، اسمزی و اکسیداتیو در شرایط شوری انجام شد.
مواد و روش‌ها: آزمایش بر اساس طرح فاکتوریل بر پایه بلوکهای کامل تصادفی با سه تکرار انجام شد. فاکتورهای شامل شوری (0، 4، 7 و 10 دسی زیمنس بر متر) و هورمون‌ها (50 میکرو‌مولار کینتین: و 1 میلی‌مولار سالیسیلیک اسید( بودند. تعداد 20 عدد بذر رقم M7 در عمق 3 سانتی-متری هر گلدان حاوی کیلوگرم پرلیت کاشته شدند. محلول‌پاشی هورمون‌ها در مرحله رشد رویشی و زایشی انجام گرفت. یک هفته پس از تیمار نهایی آزمایشات انجام گرفتند.
یافته‌ها: شوری محتوای سدیم ریشه‌ها و برگ‌ها، مقدار پراکسید هیدروژن و مالون‌دی‌آلدئید، فعالیت کاتالاز، آسکوربات پراکسیداز، سوپراکسید دیسموتاز، آلفا-توکوفرول و محتوای قندهای محلول و پرولین برگ گیاهان سویا را افزایش داد. این در حالی است که محتوای یون‌های پتاسیم، کلسیم و منیزیم، محتوای کلروفیل، شاخص سطح برگ، شاخص پایداری غشا، محتوای نسبی آب برگ و عملکرد دانه را کاهش داد. محلول‌پاشی هورمون‌ها، سبب کاهش محتوای سدیم ریشه‌ها و برگ‌ها، مقدار پراکسید هیدروژن، مالون‌دی‌آلدئید و پرولین برگ شد، در حالیکه محتوای یون‌های پتاسیم، کلسیم و منیزیم، محتوای کلروفیل، شاخص سطح برگ، فعالیت آنتی‌اکسیدان‌ها، شاخص پایداری غشا، مقدار قندهای محلول و محتوای نسبی آب برگ را بهبود بخشیدند. برتری تیمار Kin+ SA در رابطه با پارامترهای مورد بررسی به افزایش قابل توجه عملکرد بذر گیاهان سویا منجر گردید.
نتیجه‌گیری: محلول‌پاشی هورمون‌ها، از طریق کاهش سمیت یونی و بهبود فعالیت آنتی‌اکسیدان-ها، به افزایش رشد و عملکرد بذر گیاهان سویا منجر گردید

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Effects of foliar application of salicylic acid and kinetin on soybean physiological responses at different levels of salt stress

نویسندگان [English]

  • Javid Kardan 1
  • Reza Amirnia 1
  • Mahdi Ghiyasi 2
  • Leila Sharifi 3
1 Department ofPlant Production and Genetics, Faculty of Agriculture, Urmia University
2 Plant Production and Genetics
3 Computer Engineering Department, Electrical and Computer Faculty, Urmia University
چکیده [English]

Background and Objective: This research was undertaken to assess the impact of kinetin (Kin) and salicylic acid (SA) on alleviation of oxidative, ionic and osmotic stresses of different levels of salinity.
Materials and Methods: the experiment with factorial arrangements based on randomized complete block design with three replications were conducted at the research greenhouse of Urmia University. The investigated factors included salinity (0, 4, 7 and 10 dS/m) and hormones (50 μM kintin and 1 mM salicylic acid). 20 soybean seeds (cultivar M7) were planted at a depth of 3 cm in each pot containing 1 kg of perlite. Hormones were sprayed in the vegetative and reproductive stages. One week after the final treatment, tests were performed.
Results: Salinity increased the contents of roots and leaves sodium, the amount of H2O2 and malondialdehyde, the activities of catalase, ascorbate peroxidase, superoxide dismutase, α-tocopherol, and the contents of soluble sugars and proline of soybean leaves, but decreased potassium, calcium and magnesium ions, chlorophylls content, leaf area index, membrane stability index, leaf relative water content and seed yield. Foliar spray of hormones, especially Kin + SA, reduced Na+ content, H2O2 and malondialdehyde and proline content, while enhancing potassium, calcium and magnesium ions, chlorophylls content, leaf area index, antioxidants avtivities, membrane stability index, soluble sugars and leaf relative water content. These superiorities of Kin + SA treatment led to considerable improvement in seed yield of soybean.

کلیدواژه‌ها [English]

  • Antioxidants
  • Cytokinin
  • Salicylic acid
  • Salt toxicity
  • Soybean
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