بررسی توانایی تشکیل بیوفیلم توسط باکتری‌های محرک رشد بیوفیلمی در سطح ریشه گندم (Triticum aestivum L.) و تاثیر آنها بر عملکرد آن

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشگاه مراغه

2 دانشگاه تبریز

3 گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه مراغه

چکیده

اهداف: باکتریهای محرک رشد ریزوسفری جداسازی شده از گیاهان غیرزراعی به دلیل برتری در کلنیزاسیون ریشه گیاهان با تشکیل بیوفیلم و سابقه مکرر در مواجهه با شرایط نامطلوب اقلیمی می‌توانند باعث افزایش رشد گیاهان زراعی گردیده و به عنوان کاندیدای مطلوب در تولید مایه تلقیح های کارآمد مورد استفاده قرار گیرند.
مواد و روش‌ها: این آزمایش در شرایط کنترل شده با مایه‌زنی بذور گندم با نه باکتری محرک رشد (Bacillus atrophaeus 10-1، B. toyonensis 29-2، P. lautus 29-4 ، B. simplex 32-1 (B4)، B. simplex 8-3، B. toyonensis 18-2، B. zhangzhouensis 23-3 ، B. atrophaeus 39-2، B. velezensis 45-4) در قالب طرح کامل تصادفی در سه تکرار انجام گردیده و ویژگی‌های رشدی گندم در شرایط غیرتنشی مورد بررسی قرار گرفت.
یافته‌ها: نتایج مطالعات میکروسکوپی نشان دادند که باکتری‌ها مذکور می‌توانند در سطح ریشه گندم بیوفیلم با ساختارهای مختلف تشکیل دهند. صفات وزن خشک کل، وزن خشک ساقه، وزن خشک ریشه، وزن تر کل اندام هوایی، وزن تر ساقه و وزن تر سنبله، محتوای کلروفیل برگ دوم و ارتفاع گندم تحت تاثیر مایه‌زنی باکتریایی قرار گرفتند. باکتری B. toyonensis 18-2و باکتری B. zhangzhouensis 23-3 توانستند در مقایسه با تیمار بدون مایه‌زنی باکتریایی وزن خشک کل را به ترتیب 15 و 12 درصد افزایش دهند.
نتیجه‌گیری: تشکیل بیوفیلم میکروبی توسط باکتری‌های محرک رشد جدا شده از گیاهان غیرزراعی در سطح ریشه گندم نشان داد که این باکتری‌ها توانایی بهره گیری از ترشحات ریشه گندم و استقرار در ریزوسفر این گیاه می‌باشند.

کلیدواژه‌ها


عنوان مقاله [English]

Evaluation of biofilm formation ability by biofilm growth promoting bacteria at the root surface of wheat (Triticum aestivum L.) and their effect on its yield

نویسندگان [English]

  • Esmaeil Karimi 1
  • Nasser Aliasgharzad 2
  • Ezzatallah Esfandyari 3
1 University of Maragheh
2 University of Tabriz
3 University of Maragheh
چکیده [English]

Background & Objective: Rhizospheric growth-promoting-bacteria isolated from wild plants are introduced as biofilm forming PGPR. Due to their mode of action they behave as winner in plant root colonization process in the soil. Because of such advantage they are good candidates for the efficient inoculum production industries.
Materials and Methods: This experiment was performed under controlled conditions by inoculation of wheat seeds with nine biofilm forming growth promoting bacteria in a completely randomized design with three replications, and the growth characteristics of wheat were investigated under non-stress conditions.
Results: The bacterial biofilm forming on wheat root was verified under confocal microscopic imaging. From the structural point of view, the diverse biofilms were detected among the bacteria. Total dry weight, stem dry weight, root dry weight, total shoot fresh weight, stem fresh weight and spike fresh weight, second leaf chlorophyll content, and wheat height were affected by the bacterial inoculation. Bacillus toyonensis 18-2 and Bacillus zhangzhouensis 23-3 were able to increase the total dry weight by 15% and 12%, respectively; compared to control treatment.
Conclusion: The biofilm formation by PGPR isolated from non-crops on the surface of wheat root showed that these bacteria have the ability to utilize wheat root secretions and settle in its rhizosphere. Due to the positive effect of the bacteria on wheat growth, the rhizosphere of non-crop plants can be considered as a suitable source for the isolation of new PGPR. The above mentioned two bacteria can be used as a potential candidate for the future experiments.

کلیدواژه‌ها [English]

  • Bacillus
  • Root morphology
  • Confocal microscope
  • Wheat root
  • Biological Yield
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