تاثیر باکتریهای سینوریزوبیوم و سودوموناس بر صفات رشدی، جذب عناصر، عملکرد بذر و کارایی مصرف آب شنبلیله تحت تنش کمبود آب

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه علوم باغبانی، واحد علوم وتحقیقات، دانشگاه آزاد اسلامی، تهران، ایران

2 گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه تبریز

3 مرکز تحقیقات گیاهان دارویی، پژوهشکده گیاهان دارویی جهاد دانشگاهی، کرج، ایران

4 گروه علوم خاک، دانشکده کشاورزی، دانشگاه تبریز

چکیده

یکی از مهمترین عوامل کاهش تولید محصولات کشاورزی کمبود آب است. باکتریهای محرک رشد در کنترل تنش کمبود آب نقش مهمی ایفا می‌کنند. امروزه در راستای کنترل اثرات تنش و تولید محصول سالم، کاربرد کودهای زیستی از جمله باکتریهای محرک رشد مورد توجه است. تحقیق حاضر به مطالعه اثر تلقیح دو باکتری محرک رشد گیاه (Sinorhizobium meliloti وPseudomonas fluorescens) بر برخی از خصوصیات مورفوفیزیولوژیکی شنبلیله تحت تنش کم آبی می‌پردازد. بدین منظور آزمایش فاکتوریل در قالب طرح بلوکهای کامل تصادفی، که فاکتور اول تلقیح باکتریایی در چهار سطح به صورت تلقیح منفرد، تلقیح توأم آنها و شاهد بدون باکتری و فاکتور دوم شامل چهار سطح آبیاری به­ترتیب 40، 60، 80 و100 درصد ظرفیت مزرعه‌ای،  در سه تکرار اجرا شد. تیمارهای آبیاری بعد از گلدهی گیاهان تا زمان برداشت اعمال شد. نتایج نشان داد که سطح برگ، وزن تر و خشک قسمت هوایی و وزن تر و خشک ریشه، مقدار فسفر و پتاسیم، و کارآیی مصرف آب با کاربرد باکتریهای محرک رشد بطور معنی‌داری افزایش یافت در حالی‌که عملکرد بذر در کاربرد باکتریهای محرک رشد کاهش یافت.


 

کلیدواژه‌ها


عنوان مقاله [English]

The Effects of Sinorhizobium and Pseudomonas on Growth Characteristics, Nutrient Uptake, Seed Yield and Water Use Efficiency of Fenugreek under Water Deficit Stress

نویسندگان [English]

  • Ali Sharghi 1
  • Sahebali Bolandnazar 2
  • Hassan Ali Naghdi Badi 3
  • Ali Mehrafarin 3
  • Mohammad Reza Sarikhani 4
چکیده [English]

Water deficit stress limits agriculture crop production. Plant growth-promoting rhizobacteria (PGPR) play an important role in control of water deficit stress. Today, for improving of plant tolerance to stress and the production of healthy crops, the application of biofertilizers, including growth-promoting bacteria, is interest. The present study investigates the effects of two different PGPRs (Pseudomonas fluorescens and Sinorhizobium meliloti) on some physiological and morphological characteristics in fenugreek under water deficit stress. For this purpose, a factorial design based on randomized complete block design with four water deficit levels (100%, 80%, 60% and 40% FC) and four PGPR condition as control, Sinorhizobium meliloti, Pseudomonas fluorescens and combination of S. meliloti. and P. fluorescens with three replications were carried out. Irrigation treatments were applied after flowering of plants until harvesting. The results showed that leaf area, shoot and root fresh and dry weight, phosphorus and potassium content, and water use efficiency (WUE) were significantly improved by PGPR inoculation and individual use of PGPR was more effective, whereas seed yield was decreased in PGPR treated plants.
 

کلیدواژه‌ها [English]

  • Fenugreek
  • Medicinal Plant
  • PGPR
  • Sinorhizobium
  • Water deficit stress
Ahmad M, Zahir ZA, Khalid M, Nazli F and Arshad M. 2013. Efficacy of Rhizobium and Pseudomonas strains to improve physiology, ionic balance and quality of mung bean under salt-affected conditions on farmer's fields. Plant Physiology and Biochemistry, 63: 170-176.
Ajabnoor MA and Tilmisany AK. 1988. Effect of(Trigonella foenum-graceumL.)on blood glucose levels in normal and alloxan-diabetic mice. Journal of Ethnopharmacology, 22: 45-49.
Arshad M, Shaharoona B and Mahmood T. 2008. Inoculation with (Pseudomonas spp.) Containing ACC-Deaminase partially eliminates the effects of drought stress on growth, yield, and ripening of pea (Pisum sativum L.). Pedosphere, 18: 611-620.
Baker WH and Thompson TL. 1997. Determination of total nitrogen inplant samples by kjeldahl. In: Plant analysis reference procedures for the southern region of the united states; Plank, C. O (ed). US, pp:13-16.
Bartels D and Sunkar R. 2005. Drought and salt tolerance in plants. Critical Reviews in Plant Sciences, 24: 23-58.
Black CA,  Evans DD, WhiteJL, Ensminger LE and Clark FE. 1965. Methods of Soil Analysis: Part 2. Madison, WI: ASA.
Bray EA. 1997. Plant responses to water deficit. Trends in Plant Science, 2: 48-54.
Bray EA. 2004. Genes commonly regulated by water-deficit stress in Arabidopsis thaliana. Journal of Experimental Botany, 55: 2331-2341.
Bremner DC and Mulvaney JM. (1982). Total Nitrogen. In:Methods of Soil Analysis. (A. L. Page, R. H. Miller and D. R. Keaney, eds). N: 9 Part 2, American Society of Agronomy.
Cassel DK and Nielsen DR. 1990. Field capacity and available water capacity. Pp: 901-926. In: Klute A (ed). Methods of Soil Analysis. Part 1: Physical and Mineralogical Methods, 2nd ed. American Society of Agronomy and Soil Science Society of Aemrica, Madison, WI.
Chandler D, Davidson G, Grant W, Greaves J and Tatchell G. 2008. Microbial biopesticides for integrated crop management an assessment of environmental and regulatory sustainability. Trends in Food Science & Technology, 19: 275-283.
Compant S, Duffy B, Nowak J, Clément C and Barka EA. 2005. Use of plant growth-promoting bacteria for biocontrol of plant diseases, principles, mechanisms of action, and future prospects. Applied and Environmental Microbiology, 71: 4951-4959.
Dimkpa C, Weinand T and Asch F. 2009. Plant–rhizobacteria interactions alleviate abiotic stress conditions. Plant,Cell &Environment, 32: 1682-1694.
Dodd IC and Ryan AC. 2016.  Whole‐plant physiological responses to water‐deficit stress. eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net.
El-Tarabily KA and Sivasithamparam K. 2006. Non-streptomycete actinomycetes as biocontrol agents of soil-borne fungal plant pathogens and as plant growth promoters. Soil Biology and Biochemistry, 38: 1505-1520.
Fernández-Aparicio M, Emeran AA and Rubiales D. 2008. Control of Orobanche crenata inlegumes intercropped with fenugreek (Trigonella foenum-graecumL.). Crop Protection, 27: 653-659.
Gee GW and Bauder JW, 1986. Particle size analysis. Pp. 383-411.  In: Klute A (ed). Methods of Soil Analysis. Part 1: Physical and Mineralogical Methods, 2nd ed. American Society of Agronomy and Soil Science Society of Aemrica, Madison, WI.
Govindasamy V, Senthilkumar M, Gaikwad K and Annapurna K. 2008. Isolation and characterization of ACC deaminase gene from two plant growth-promoting rhizobacteria. Current microbiology, 57: 312-317.
Havlin JL and Soltanpour P. 1980. A nitric acid plant tissue digest method for use with inductively coupled plasma spectrometry 1. Communications in Soil Science & Plant Analysis, 11: 969-980.
He Z and Yang X. 2007. Role of soil rhizobacteria in phytoremediation of heavy metal contaminated soils. Journal of Zhejiang University Science, B, 8: 192-207.
Heidari M and Golpayegani A. 2012. Effects of water stress and inoculation with plant growth promoting rhizobacteria (PGPR) on antioxidant status and photosynthetic pigments in basil (Ocimum basilicum L.). Journal of the Saudi Society of Agricultural Sciences, 11: 57-61.
Hummel I, Pantin F, Sulpice R, Piques M, Rolland G, Dauzat M, Christophe A, Pervent M, Bouteillé M and Stitt M. 2010. Arabidopsis plants acclimate to water deficit at low cost through changes of carbon usage: an integrated perspective using growth, metabolite, enzyme, and gene expression analysis. Plant Physiology, 154: 357-372.
Irankhah S, Ganjali A, Lahooti M and Moshregi, M. 2017. The effect of Pseudomonas putida and  Glomus intraradices on some morphologiacal and biochemichal traits of  Trigonella foenum-graecum L. Journal of Horticultural Science, 20(1): 112-121. (In Persian).
Jaleel CA, Manivannan P, Sankar B, Kishorekumar A, Gopi R, Somasundaram R and Panneerselvam R. 2007. Pseudomonas fluorescens enhances biomass yield and ajmalicine production in Catharanthus roseus under water deficit stress. Colloids and Surfaces B: Biointerfaces, 60: 7-11.
Karimi HR and Roosta H. 2014. Evatuation of inter-specific hybrid of P. atlantica and P. vera L. cv.‘Badami riz-e-Zarand’as pistachio rootstock to salinity stress according to some growth indicesand eco -physiology and bichemichal parameters. Journal of Stress Physiology & Biochemistry, 10.
Karlidag H, Esitken A, Turan M and Sahin F. 2007. Effects of root inoculation of plant growth promoting rhizobacteria (PGPR) on yield, growth and nutrient element contents of leaves of apple. Scientia Horticulturae, 114: 16-20.
Kloepper J, Reddy M, Rodríguez-Kabana R, Kenney D, Kokalis-Burelle N, Martinez-Ochoa N and Vavrina C. 2004. Application for rhizobacteria in transplant production and yield enhancement. Acta Horticulturae, 61: 217-230.
Knudsen D, Peterson GA and Pratt PF. 1982. Lithium, sodium and potassium. Pp: 225-246. In: Page AL, (ed). Methods of Soil Analysis. Part 2: Chemical and Microbiological Properties, 2nd ed. American Society of Agronomy and Soil Science Society of Aemrica, Madison, WI.
Lee KJ, Oh BT and Seralathan KK. 2013. Advances in Plant Growth Promoting Rhizobacteria for biological control of plant diseases, Bacteria in Agrobiology: Disease Management. Springer, pp: 1-13.
Lorki S and Akhgar A. 2014. The effect of Sinorhizobium sp on yield, nodulusation and nitrogen fixation in fuengreek. Soil biology, 2(2): 137-148. (In Persian).
Mayak S, Tirosh T and Glick BR. 2004a. Plant growth-promoting bacteria confer resistance in tomato plants to salt stress. Plant Physiology and Biochemistry, 42: 565-572.
Mayak S, Tirosh T and Glick BR. 2004b. Plant growth-promoting bacteria that confer resistance to water stress in tomatoes and peppers. Plant Science, 166: 525-530.
Mclean EO. 1982. Soil pH lime requirement. Pp. 199-223. In: Page AL, (ed). Methods of Soil Analysis. Part 2: Chemical and Microbiological Properties, 2nd ed. American Society of Agronomy and Soil  Science Society of Aemrica, Madison, WI.
Miraldi E, Ferri S and Mostaghimi V. 2001. Botanical drugs and preparations in the traditional medicine of West Azerbaijan (Iran). Journal of Ethnopharmacology, 75: 77-87.
Mishra M, Kumar U, Mishra PK and Prakash V. 2010. Efficiency of plant growth promoting rhizobacteria for the enhancement of Cicer arietinum L. growth and germination under salinity. Advances in Biology Research, 4: 92-96.
Nelsons BW and Sommers LE. 1982. Total carbpn, organic carbon, and organic matter, Pp. 539-579. In: Page AL, (ed). Methods of Soil Analysis. Part 2: Chemical and Microbiological Properties, 2nd ed. American Society of Agronomy and Soil  Science Society of Aemrica, Madison, WI.
Olsen SR and Sommers LE. 1982. Phosphorus. Pp. 403-130. In: Page AL, (ed) Methods of Soil Analysis. Part 2: Chemical and Microbiological Properties, 2nd ed. American Society of Agronomy and Soil  Science Society of Aemrica, Madison, WI.
Ordookhani K, Khavazi K, Moezzi A and Rejali F. 2010. Influence of PGPR and AMF on antioxidant activity, lycopene and potassium contents in tomato. African Journal of Agricultural Research, 5: 1108-1116.
Rhoades JD. 1986. Soluble salts. Pp. 167-179. In: Page AL, (ed). Methods of Soil Analysis. Part 2: Chemical and Microbiological Properties, 2nd ed. American Society of Agronomy and Soil  Science Society of Aemrica, Madison, WI.
Ryu CM, Farag MA, Hu CH, Reddy MS, Wei HX, Paré PW and Kloepper JW. 2003. Bacterial volatiles promote growth in Arabidopsis. Proceedings of the National Academy of Sciences, 100: 4927-4932.
Salekdeh GH, Reynolds M, Bennett J and Boyer J. 2009. Conceptual framework for drought phenotyping during molecular breeding. Trends in Plant Science, 14: 488-496.
Sandhya V, Ali SZ, Grover M, Reddy G and Venkateswarlu B. 2010. Effect of plant growth promoting Pseudomonas spp. on compatible solutes, antioxidant status and plant growth of maize under drought stress. Plant Growth Regulation, 62: 21-30.
Sharghi A, Bolandnazar S, Naghdi Badi H, Mehrafarin A, and Sarikhani MR. 2017.The effects of plant growth promoting rhizobacteria (PGPR) on growth characteristics of fenugreek under water deficit stress. Advances in  Bioresearch, 8(5):96‐101.
Sharma R and Raghuram T. 1990. Hypoglycaemic effect of fenugreek seeds in non-insulin dependent diabetic subjects. Nutrition Research, 10: 731-739.
Shaukat K, Affrasayab S and Hasnain S. 2006. Growth Responses ofTriticum aestivum to Plant Growth Promoting Rhizobacteria used as a biofertilizer. Research Journal of Microbiology, 1: 330-338.
Smith M. 2003. Therapeutic applications of fenugreek. Alternative Medicine Review, 8: 20-27.
Srivastava S, Yadav A, Seem K, Mishra S, Chaudhary V and Nautiyal C. 2008. Effect of high temperature on Pseudomonas putida NBRI0987 biofilm formation and expression of stress sigma factor RpoS. Current Microbiology, 56: 453-457.
Van Loon L. 2007. Plant responses to plant growth-promoting rhizobacteria. European Journal of Plant Pathology, 119: 243-254.
Vessey JK. 2003. Plant growth promoting rhizobacteria as biofertilizers. Plant and Soil, 255: 571-586.
Warke VB, Deshmukh TA and Patil VR. 2011. Development and validation of RP-HPLC method for estimation of diosgenin in pharmaceutical dosage form. Asian Journal of Pharmaceutical and Clinical Research, 4: 126-128.
Yang J, Kloepper JW and Ryu CM. 2009. Rhizosphere bacteria help plants tolerate abiotic stress. Trends in Plant Science, 14: 1-4.
Zasoski R and Burau R. 1977. A rapid nitric‐perchloric acid digestion method for multi‐element tissue analysis. Communications in Soil Science & Plant Analysis, 8: 425-436.
Żuk-Gołaszewska K, Wierzbowska J and Bieńkowski T. 2015. Effect of potassium fertilization, rhizobium inoculation and water deficit on the yield and quality of fenugreek seeds. Journal of Elementology, 20(2): 513-524.