بررسی زنده‌مانی باکتری Enterobacter cloacae در چند حامل جامد و اثر زادمایه‌های تهیه شده بر جوانه‌زنی و رشد گندم

نوع مقاله : مقاله پژوهشی

نویسندگان

1 بیولوژی و بیوتکنولوژی خاک، دانشکده کشاورزی، دانشگاه تبریز

2 شیمی و حاصلخیزی خاک، دانشکده کشاورزی، دانشگاه تبریز

چکیده

 در کشاورزی پایدار کودهای زیستی جایگاه ویژه‌ای دارند. به همین خاطر برای عرضه آن‌ها از حامل‌های مختلف برای حفظ ماندگاری و زنده‌مانی باکتری استفاده می‌شود. این تحقیق با هدف بررسی زنده‌مانی باکتری Enterobactercloacae بر روی حامل‌های مختلف جامد در مدت یک سال صورت گرفت. حامل‌های جامد، شامل 10 تیمار باگاس، پیت، هیدروچار، بیوچار، خاک‌اره و پرلیت به‌صورت منفرد و مخلوط آن‌ها با پرلیت با نسبت وزنی (1:1) بودند. در این بررسی، زادمایه‌های باکتریایی تهیه شده با جمعیت اولیه یکسان (CFU/g109) پس از نگهداری در دمای اتاق از نظر توان ماندگاری و زنده‌مانی باکتری مورد مقایسه قرار گرفتند. جمعیت باکتری در زمان­های 0، 15، 30، 60، 90، 120، 180، 270و 365 روز شمارش شد. برای شمارش باکتری‌های زنده در حامل‌های میکروبی، بعد از تهیه سری‌های رقت از روش­ شمارش در کشت نواری درون یک پلیت استفاده شد. در این تحقیق اثرات زادمایه‌های تهیه شده بر جوانه‌زنی و رشد گیاهچه‌های گندم در شرایط استریل و به روش کشت در پلیت شیشه‌ای، در پایان ماه چهارم بررسی شد. در این دو روش خصوصیاتی از قبیل طول ساقه و ریشه، وزن تر و خشک ساقه و ریشه و وزن تر و خشک کل ساقه و ریشه اندازه‌گیری شد. نتایج حاصل از شمارش باکتری‌ها نشان داد که از میان حامل‌های مورد آزمایش، بیشترین جمعیت شمارش شده بعد از گذشت یک سال در حامل باگاس (CFU/g 109) و کمترین جمعیت شمارش شده در خاک‌اره به دست آمد بطوریکه بعد از گذشت 6 ماه هیچ جمعیت زنده باکتری در خاک‌اره شمارش نشد. همچنین نتایج حاصل از تست جوانه‌زنی و رشد گیاهچه‌های گندم نشان داد که زادمایه‌های هیدروچار و باگاس+ پرلیت در کشت پلیت نتایج تکرارپذیری داشتند و در هر دو حالت در اغلب ویژگی‌های اندازه‌گیری شده، میانگین‌های بهتری را به خود اختصاص داده بودند. در پایان با توجهبه نتایج این آزمایش و راحتی و در دسترس بودن حامل‌های مورد استفاده، حامل باگاس+ پرلیت توصیه می‌شود.
 
 

کلیدواژه‌ها

عنوان مقاله [English]

Study the Survival of Enterobacter cloacae Bacteria in Several Solid Carriers and Effect of Prepared Inoculants on Ggermination and Growth of Wheat

نویسندگان [English]

  • Fatemeh Ghasemi Piranlo 1
  • Mohammad Reza sarikhani 1
  • Nosrat Allah Najafi 2
چکیده [English]

         Biofertilizers play major role in sustainable agriculture. Therefore, to provide them, different carriers are used to increase the longevity and survival of the bacteria. The aim of this study was to investigate the survival of Enterobacter cloacae bacteria on different solid carriers during one year. Solid carriers consist of 10 treatments of bagasse, peat, hydrochar, biochar, sawdust and perlite as a single treatment and mixed with perlite (in a ratio of 1:1). In this study, bacterial inoculants prepared with the initial population (109 CFU/g) after storage at room temperature were compared for the survival of the bacteria. The bacterial population was counted at 0, 15, 30, 60, 90, 120, 180, 270 and 365 days. For counting the bacteria in microbial carriers, after dilution series preparation, bacterial suspension was used in strip culture in a plate. In this research, the effects of prepared inoculants on germination and growth of wheat seedlings in sterile conditions in a plate culture at the end of the fourth month were investigated. In these method, characteristics such as shoot and root length, the wet and dry weight of shoot and root, total wet and dry weight of shoot and root were measured.The results of bacterial count showed that among the tested carriers, the most population counted after one year in bagasse carrier (109 CFU/g) and the lowest population was counted in the sawdust, so that after 6 months no alive cells of bacteria were counted in sawdust. Also, the results of germination test and growth wheat seedling growth showed that in most of the measured characteristics the hydrochar and bagasse + perlite in both plate experiment, had reproducible results and they had better means. Concluding, according to the results of this experiment and the convenience and availability of the carriers, bagasse + perlite carrier had the best results in increasing the survival of the bacteria and we suggest this kind of carrier.


 

 
 
 

کلیدواژه‌ها [English]

  • Bacterial survival
  • Biofertilizers
  • Enterobacter cloacae
  • Solid Carrier
  • Wheat

مراجع

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دانش کشاورزی وتولید پایدار
دوره 29، شماره 3
مهر 1398
صفحه 167-180

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