اثر قارچ اندوفیت Piriformospora indica بر شاخص‌های رشد و جذب عناصر در گیاه آنیسون (Pimpinella anisum) در شرایط تنش کم‌آبی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانش آموخته کارشناسی ارشد بیولوژی و بیوتکنولوژی خاک، دانشکده کشاورزی، دانشگاه تبریز

2 استاد بیولوژی و بیوتکنولوژی خاک، دانشکده کشاورزی، دانشگاه تبریز

3 استاد اکوفیزیولوژی گیاهی، دانشکده کشاورزی، دانشگاه تبریز

4 دانشجوی دکترای بیولوژی و بیوتکنولوژی خاک، دانشکده کشاورزی، دانشگاه تبریز

چکیده

وضعیت تغذیه‌ای گیاهان دارویی به دلیل دخالت مستقیم آنها در رژیم غذایی انسان دارای اهمیت می‌باشد ازطرفی تنش کمبود آب ممکن است بر میزان جذب عناصر مغذی و تولید زیست توده در این گیاهان تاثیر منفی داشته باشد. شواهدی وجود دارد که نشان می دهد قارچ اندوفیت Piriformospora indica تنش کمبود آب را در گیاهان کاهش و جذب مواد مغذی را بهبود می بخشد. در این پژوهش آزمایش گلدانی در قالب طرح کاملاً تصادفی به صورت فاکتوریل با دو فاکتور شامل دو سطح قارچ P. indica (تلقیح و عدم­تلقیح) و سه سطح رطوبت خاک شامل تنش شدید %FC(W2) 40-50، تنش متوسط (W1)%FC 60-70 و بدون تنش (W0)%FC 80-90 در چهار تکرار اجرا شد. نتایج نشان داد که اثرمتقابل تنش  کم آبی × قارچ  بر روی همه خصوصیات مورفولوژیک به غیر از ارتفاع گیاه معنی‌دار بود (05/0 P<). در شرایط تنش کم‌آبی شدید (W2)، تلقیح قارچ باعث افزایش وزن خشک بخش هوایی (4/22%) و ریشه (4/8%)، شاخص کلروفیل (4/9%)، فلورسانس کلروفیل (5/5%)، غلظت فسفر بخش هوایی (3/61) و ریشه (6/65%)، غلظت پتاسیم هوایی (6/22%) و ریشه (6/24%)، درصد کلنیزاسیون ریشه (100%)  و عملکرد بذر (1/47%) در مقایسه با گیاهان شاهد بدون تلقیح شد. همچنین بیشترین مقدار شاخص‌های فوق در سطح رطوبتی بدون تنش (W0) و حضور قارچ  P. indica به دست آمد.
 

کلیدواژه‌ها


عنوان مقاله [English]

Effects of Endophytic Fungus Piriformospora indica on Growth Indices and Nutrient Uptake by Anise Plant (Pimpinella anisum) under Water Deficit Stress Conditions

نویسندگان [English]

  • Solmaz Abdollahi 1
  • Naser Ali Asgharzad 2
  • Saeed Zahtab Selmasi 3
  • Bahman Khoshru 4
چکیده [English]

Nutritional status of medicinal plants is important due to their direct involvement in human dietary. Also, water deficit stress may adversely affect nutrient uptake and biomass production of these plants. There are evidences indicating that endophytic fungusPiriformospora indica improves nutrients uptake and mitigates water deficit stress in plants. In this study, a pot culture experiment was conducted in a completely randomized design with factorial arrangement with two factors including two levels of P. indica (inoculated and non-inoculated) and soil moisture with three levels of 0.8-0.9 FC (W0), 0.6-0.7 FC (W1) and 0.4-0.5 FC (W2) with four replications. The results showed that the interaction effect of water deficit × fungus on all morphological characteristics except plant height was significant (P <0.05). In severe water deficit stress (W2), fungal inoculation leads to increase in dry weight of shoot (22.4%) and root (8.4%), chlorophyll index (4.9%), chlorophyll fluorescence (5.5%), shoot (61.3%) and root (65.6%) P concentration, shoot (22.6%) and root (24.6%) K concentration, root colonization (100%) and seed yield (47.1%) compared to the un-inocultaed controls. Although the highest values of above mentioned traits were recorded at the highest moisture level (W0) in the presence of P. indica.
 

کلیدواژه‌ها [English]

  • Anise
  • Medicinal Plants
  • Nutrients
  • Root Colonization
  • Water Deficit Stress
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