شناسایی مولکولی Artemia franciscana Kellogg 1906 در دریاچه بختگان، استان فارس

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشکده علوم دانشگاه ارومیه و باشگاه پژوهشگران جوان

2 دانشکده علوم دانشگاه ارومیه

3 پژوهشکده آرتمیا و جانوران آبزی دانشگاه ارومیه

4 دانشگاه آزاد اسلامی واحد بندرعباس

چکیده

 آرتمیا سخت پوست کوچکی با ارزش اقتصادی بالاست که مدل تحقیقاتی بسیار مناسبی برای محققان می باشد. این موجود با تحمل محدوده های بسیار متنوع از شرایط بسیار مختلف اکولوژیک در بیش از 600 نقطه زمین و نیز 18 زیستگاه در ایران توزیع شده است. دریاچه بختگان در استان فارس یکی از زیستگاههای طبیعی آرتمیای پارتنوژنز در ایران می باشد. با توجه به وجود یک آرتمیای دو جنسی ناشناخته در این دریاچه، گونه این جمعیت غیر بومی مورد تحقیق قرار گرفت. بدین منظور 4 مارکر مولکولی مختلف شامل دو ژن Na/K ATPase، 16S-12S با روش پی سی
آر- آر اف ال پی[1] و دو ناحیه COI و  HSP26به روش تعیین توالیمورد تحقیق قرار گرفت. DNA  مورد نیاز از نمونه های سیست جمع آوری شده از دریاچه و همچنین آرتمیای بالغ پرورش یافته در آزمایشگاه تهیه شد. نتایج ضمن تاکید بر توانایی روشهای مولکولی جهت شناسایی یک گونه ناشناخته، این گونه را به عنوان A. franciscana شناسایی نمود که از نظر توالی ژنهای سکانس شده تنوع ژنتیکی زیادی با تمامی نمونه های ثبت شده در بانک ژنی دارد.


کلیدواژه‌ها


عنوان مقاله [English]

Molecular Characterization Artemia Franciscana Kellogg 1906 in Bakhtegan Lake, Fars Province

نویسندگان [English]

  • S Shafaie 1
  • S Zare 2
  • R Manaffar 3
  • A Falahati 4
چکیده [English]

Artemia is a small crustacean with high commercial value that provides a valuable model organism for researchers. This creature was dispersed to more than 600 and 18 sites over the world and Iran, respectively, by tolerating extreme range of different environmental conditions. Bakhtegan Lake is one the natural parthenogenetic Artemia habitat in Iran. Due to occurrence of an unknown bisexual Artemia in Bakhtegan Lake, the species of this un-endemic Artemia was inferred. In this regard, four different molecular markers as Na/K ATP-ase, 12S-16Sby PCR-RFLP technique and COI, HSP26 by were studied. The DNA was provided from the harvested cysts and reared adult Artemia in laboratory conditions. The results with emphasizing to ability of molecular techniques for identifying unknown species characterized the new population as A. franciscana. These analyses also revealed a molecular diversity between the sequenced genes with the all data found in the Genbank. 

کلیدواژه‌ها [English]

  • Artemia
  • Parthenogenetic
  • Bakhtegan Lake
  • Molecular markers
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